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AKR1B1 recombinant protein :: Aldose reductase Recombinant Protein

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Catalog # MBS203284
Unit / Price
  0.1 mg  /  $255 +1 FREE 8GB USB
  0.5 mg  /  $535 +1 FREE 8GB USB
SDS-Page
Product Name

Aldose reductase (AKR1B1), Recombinant Protein

Popular Item
Also Known As

Aldo-keta reductase family 1, member B1

Product Synonym Names
Aldo-keto reductase family1 member B1; AKR1B1; ADR; ALDR1; ALR2; AR; Aldo-keto reductase family1 member B1; Aldose reductase; Aldo-keto reductase family1; member B1 AKR1B 1; Aldehyde reductase; aldehyde reductase 1; aldo-keto reductase family 1; member B1 aldr 1; aldr-1; Lii5-2 CTCL tumor antigen; low Km aldose reductase.
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Sequence Length
316
Sequence
MASRLLLNNG AKMPILGLGT WKSPPGQVTE AVKVAIDVGY RHIDCAHVYQ NENEVGVAIQ EKLREQVVKR EELFIVSKLW CTYHEKGLVK GACQKTLSDL KLDYLDLYLI HWPTGFKPGK EFFPLDESGN VVPSDTNILD TWAAMEELVD EGLVKAIGIS NFNHLQVEMI LNKPGLKYKP AVNQIECHPY LTQEKLIQYC QSKGIVVTAY SPLGSPDRPW AKPEDPSLLE DPRIKAIAAK HNKTTAQVLI RFPMQRNLVV IPKSVTPERI AENFKVFDFE LSSQDMTTLL SYNRNWRVCA LLSCTSHKDY PFHEEF
OMIM
J04795 mRNA
3D Structure
ModBase 3D Structure for P15121
Host
E Coli
Purity/Purification
> 95% by SDS-PAGE
Form/Format
Liquid. In 20 mM Tris-HCl Buffer (pH 8.0) containing 1mM DTT, 10% glycerol
Concentration
1 mg/ml (determined by Bradford assay) (lot specific)
Antigen Species
Human
Endotoxin Level
< 1.0 EU per 1 microgram of protein (determined by LAL method)
Biological Activity
Specific activity is >800 pmol/min/ug, and is defined as the amount of enzyme that catalyze the reduction of 1.0 pmole DL-glyceraldehyde in the presence of NADPH per minute at pH7.0 at 37C.
Preparation and Storage
Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
Other Notes
Small volumes of AKR1B1 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forAKR1B1purchase
Any study of glucose metabolism can not bypass the aldose reductase AKR driven glucose to sorbitol step. This enzyme provides critical energy for multiple species, including human, tissues, such as liver and seminal vesicles. In diabetes, this enzyme is either unregulated or has increased activity. Thus studies of genome sequence and expression are hot topics. To aid in research, MyBiosource has found a recombinant protein for human also reductase. Like all our recombinant proteins, this purified AKR1B1 is ideal generating monoclonal or polyclonal species antibodies for use as primary antibody in lab developed ELISA kits. Guaranteed 95% pure by SDS PAGE and BLAST basic local alignment tool verified, you can confidently use our AKR1B1 recombinant protein in all your critical cell or molecular biology arrays. View our resources page online to view related products and reagents, like human recombinant aldehyde reductase or aldo keto reductase. Visit us at MyBiosource.com.
Related Product Information for
AKR1B1 recombinant protein
Aldose reductase (AKR1B1) is a member of the aldo-keto reductase (AKR) superfamily and catalyzes the NADPH-dependent reduction of a wide variety of carbonyl-containing compounds to their corresponding alcohols. This protein is implicated in the development of diabetic complications by catalyzing the reduction of glucose to sorbitol. Recombinant Aldose reductase (AKR1B) protein was expressed in E Coli and purified by using conventional chromatography techniques.
Product Categories/Family for AKR1B1 recombinant protein
Applications Tested/Suitable for AKR1B1 recombinant protein
SDS-PAGE

NCBI/Uniprot data below describe general gene information for AKR1B1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
35.8kDa (316aa), confirmed by MALDI-TOF. [Similar Products]
NCBI Official Full Name
aldose reductase isoform 1
NCBI Official Synonym Full Names
aldo-keto reductase family 1 member B
NCBI Official Symbol
AKR1B1  [Similar Products]
NCBI Official Synonym Symbols
AR; ADR; ALR2; ALDR1
  [Similar Products]
NCBI Protein Information
aldose reductase
UniProt Protein Name
Aldose reductase
UniProt Synonym Protein Names
Aldehyde reductase; Aldo-keto reductase family 1 member B1
Protein Family
UniProt Gene Name
AKR1B1  [Similar Products]
UniProt Synonym Gene Names
ALDR1; AR  [Similar Products]
NCBI Summary for AKR1B1
This gene encodes a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. This member catalyzes the reduction of a number of aldehydes, including the aldehyde form of glucose, and is thereby implicated in the development of diabetic complications by catalyzing the reduction of glucose to sorbitol. Multiple pseudogenes have been identified for this gene. The nomenclature system used by the HUGO Gene Nomenclature Committee to define human aldo-keto reductase family members is known to differ from that used by the Mouse Genome Informatics database. [provided by RefSeq, Feb 2009]
UniProt Comments for AKR1B1
Catalyzes the NADPH-dependent reduction of a wide variety of carbonyl-containing compounds to their corresponding alcohols with a broad range of catalytic efficiencies.
Product References and Citations for AKR1B1 recombinant protein
Ruiz FX., et al: (2009) Chem Biol Interact.178 (1-3); Donaqhue KC., et al: Diabet Med. 22 (10):1315-20;

Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Products associated withAKR1B1 recombinant protein
 Reference Product  PubMed Publications
 SORD recombinant protein  >3 publications with AKR1B1 and SORD
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