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AKT1 elisa kit :: Bovine RAC-alpha serine/threonine-protein kinase ELISA Kit

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Catalog # MBS2886426
Unit / Price
  48-Strip-Wells  /  $400 +1 FREE 8GB USB
  96-Strip-Wells  /  $530 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,305 +2 FREE 8GB USB
  10x96-Strip-Wells  /  $4,250 +5 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

RAC-alpha serine/threonine-protein kinase (AKT1), ELISA Kit

Full Product Name

Bovine RAC-alpha serine/threonine-protein kinase ELISA Kit

Product Synonym Names
RAC-alpha serine/threonine-protein kinase; Protein kinase B; PKB; Protein kinase B alpha; PKB alpha; RAC-PK-alpha; AKT1; PKB; 2.7.11.1
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
3D Structure
ModBase 3D Structure for Q01314
Species Reactivity
Specificity
This assay recognizes recombinant and natural bovine RAC-alpha serine/threonine-protein kinase. No significant cross-reactivity or interference was observed.
Samples
serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids
Detection Range
0.31 ng/mL -20.0 ng/mL
Sensitivity
<0.099 ng/mL
Preparation and Storage
For long term storage, please store the entire kit at -20 degree C.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of AKT1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forAKT1purchase
MBS2886426 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the RAC-alpha serine/threonine-protein kinase (AKT1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing AKT1. The ELISA analytical biochemical technique of the MBS2886426 kit is based on AKT1 antibody-AKT1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect AKT1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, AKT1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
AKT1 elisa kit
Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of bovine RAC-alpha serine/threonine-protein kinase concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Typical Testing Data/Standard Curve (for reference only) of AKT1 elisa kit
AKT1 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS2886426. Click to request current manual
NCBI/Uniprot data below describe general gene information for AKT1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
55,748 Da
NCBI Official Full Name
RAC-alpha serine/threonine-protein kinase
NCBI Official Symbol
NCBI Official Synonym Symbols
NCBI Protein Information
RAC-alpha serine/threonine-protein kinase
UniProt Protein Name
RAC-alpha serine/threonine-protein kinase
UniProt Synonym Protein Names
Protein kinase B; PKB; Protein kinase B alpha; PKB alpha; RAC-PK-alpha
UniProt Gene Name
UniProt Synonym Gene Names
PKB; PKB; PKB alpha  [Similar Products]
UniProt Entry Name
AKT1_BOVIN
UniProt Comments for AKT1
Akt1: an oncogenic AGC kinase that plays a critical role in regulating cell survival and metabolism in many different signaling pathways. Dual phosphorylation is required for its activation. T308 is phosphorylated by PDK1 in the PI3 kinase pathway, and S473 is phosphorylated by mTOR in the mTORC2 pathway. The 'Lys-63'-linked ubiquitination of AKT1 by TRAF6 is important for its translocation to the plasma membrane, phosphorylation, and activation. When Akt is fully phosphorylated it translocates into the nucleus, undergoes 'Lys-48'-polyubiquitination catalyzed by TTC3, leading to its proteosomal degradation. Hyperactive or overexpressed in a number of cancers including breast, prostate, lung, pancreatic, liver, ovarian and colorectal. Over 160 protein substrates are known including many that regulate transcription, metabolism, apoptosis, cell cycle, and growth.

Protein type: Kinase, protein; Protein kinase, Ser/Thr (non-receptor); Protein kinase, AGC; EC 2.7.11.1; Oncoprotein; AGC group; AKT family

Cellular Component: cytoplasm; intracellular; nucleus; plasma membrane

Molecular Function: ATP binding; protein binding; protein serine/threonine kinase activity

Biological Process: carbohydrate transport; glucose metabolic process; glycogen biosynthetic process; negative regulation of caspase activity; peptidyl-serine phosphorylation; phosphorylation; positive regulation of endothelial cell proliferation; protein amino acid phosphorylation; regulation of cell migration; regulation of translation
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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