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anti-AKT1 antibody :: Rabbit AKT1-S473 Antibody

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Catalog # MBS128348
Unit / Price
  0.05 mL  /  $265 +1 FREE 8GB USB
  0.1 mL  /  $380 +1 FREE 8GB USB
  0.2 mL  /  $555 +1 FREE 8GB USB
Western Blot (WB)
Product Name

AKT1-S473, Antibody

Popular Item
Also Known As

Phospho-AKT1-S473 Polyclonal Antibody

Product Synonym Names
Phospho-AKT1-S473: AKT1; AKT; MGC99656; PKB; PKB-ALPHA; PRKBA; RAC; RAC-ALPHA
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
480
OMIM
phenotype 615109
3D Structure
ModBase 3D Structure for P31749
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Purity/Purification
Affinity Purification
Concentration
1mg/ml (lot specific)
Species
Human
Route
Synthetic Peptide
Immunogen
A phospho specific peptide corresponding to residues surrounding S473 of human AKT1
Calculated Molecular Weight
56kDa
Preparation and Storage
Store at -20 degree C (regular) or -80 degree C (long term). Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Other Notes
Small volumes of anti-AKT1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-AKT1 antibody
The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Mutations in this gene have been associated with the Proteus syndrome. Multiple alternatively spliced transcript variants have been found for this gene.
Product Categories/Family for anti-AKT1 antibody
Applications Tested/Suitable for anti-AKT1 antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-AKT1 antibody
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200

Western Blot (WB) of anti-AKT1 antibody
Western blot analysis of extracts from NIH/3T3 using Phospho-AKT1-S473 antibody.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
anti-AKT1 antibody Western Blot (WB) (WB) image
NCBI/Uniprot data below describe general gene information for AKT1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
480
NCBI Official Full Name
RAC-alpha serine/threonine-protein kinase
NCBI Official Synonym Full Names
v-akt murine thymoma viral oncogene homolog 1
NCBI Official Symbol
NCBI Official Synonym Symbols
AKT; PKB; RAC; CWS6; PRKBA; PKB-ALPHA; RAC-ALPHA
  [Similar Products]
NCBI Protein Information
RAC-alpha serine/threonine-protein kinase; AKT1m; PKB alpha; RAC-PK-alpha; proto-oncogene c-Akt; protein kinase B alpha; rac protein kinase alpha
UniProt Protein Name
RAC-alpha serine/threonine-protein kinase
UniProt Synonym Protein Names
Protein kinase B; PKB; Protein kinase B alpha; PKB alpha; Proto-oncogene c-Akt; RAC-PK-alpha
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
PKB; RAC; PKB; PKB alpha  [Similar Products]
UniProt Entry Name
AKT1_HUMAN
NCBI Summary for AKT1
The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Mutations in this gene have been associated with the Proteus syndrome. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2011]
UniProt Comments for AKT1
Akt1: an oncogenic AGC kinase that plays a critical role in regulating cell survival and metabolism in many different signaling pathways. Dual phosphorylation is required for its activation. T308 is phosphorylated by PDK1 in the PI3 kinase pathway, and S473 is phosphorylated by mTOR in the mTORC2 pathway. The 'Lys-63'-linked ubiquitination of AKT1 by TRAF6 is important for its translocation to the plasma membrane, phosphorylation, and activation. When Akt is fully phosphorylated it translocates into the nucleus, undergoes 'Lys-48'-polyubiquitination catalyzed by TTC3, leading to its proteosomal degradation. Hyperactive or overexpressed in a number of cancers including breast, prostate, lung, pancreatic, liver, ovarian and colorectal. Over 160 protein substrates are known including many that regulate transcription, metabolism, apoptosis, cell cycle, and growth.

Protein type: Protein kinase, AGC; EC 2.7.11.1; Oncoprotein; Protein kinase, Ser/Thr (non-receptor); Kinase, protein; AGC group; AKT family

Chromosomal Location of Human Ortholog: 14q32.32

Cellular Component: microtubule cytoskeleton; nucleoplasm; mitochondrion; cytoplasm; plasma membrane; spindle; intercellular junction; nucleus; cytosol

Molecular Function: protein serine/threonine kinase activity; identical protein binding; protein binding; enzyme binding; phosphatidylinositol-3,4,5-triphosphate binding; protein kinase C binding; nitric-oxide synthase regulator activity; protein serine/threonine/tyrosine kinase activity; kinase activity; phosphatidylinositol-3,4-bisphosphate binding; ATP binding; protein kinase activity

Biological Process: negative regulation of JNK cascade; regulation of myelination; positive regulation of nitric oxide biosynthetic process; nerve growth factor receptor signaling pathway; protein ubiquitination; glucose homeostasis; protein amino acid phosphorylation; G1/S-specific positive regulation of cyclin-dependent protein kinase activity; regulation of cell migration; germ cell development; positive regulation of glucose import; cell projection organization and biogenesis; protein catabolic process; maternal placenta development; response to food; platelet activation; glycogen biosynthetic process; fibroblast growth factor receptor signaling pathway; positive regulation of nitric-oxide synthase activity; positive regulation of blood vessel endothelial cell migration; glucose metabolic process; positive regulation of lipid biosynthetic process; positive regulation of cell growth; insulin-like growth factor receptor signaling pathway; cellular response to insulin stimulus; response to heat; T cell costimulation; striated muscle cell differentiation; positive regulation of fat cell differentiation; negative regulation of protein kinase activity; positive regulation of transcription factor activity; positive regulation of endothelial cell proliferation; positive regulation of transcription from RNA polymerase II promoter; response to oxidative stress; regulation of nitric-oxide synthase activity; negative regulation of apoptosis; negative regulation of autophagy; negative regulation of fatty acid beta-oxidation; translation; apoptosis; protein amino acid autophosphorylation; positive regulation of cellular protein metabolic process; positive regulation of glycogen biosynthetic process; regulation of glycogen biosynthetic process; negative regulation of cell size; glucose transport; negative regulation of caspase activity; signal transduction; nitric oxide metabolic process; regulation of translation; apoptotic mitochondrial changes; protein kinase B signaling cascade; nitric oxide biosynthetic process; inflammatory response; cell differentiation; activated T cell apoptosis; aging; negative regulation of proteolysis; epidermal growth factor receptor signaling pathway; phosphoinositide-mediated signaling; myelin maintenance in the peripheral nervous system; protein modification process; endocrine pancreas development; positive regulation of peptidyl-serine phosphorylation; osteoblast differentiation; G-protein coupled receptor protein signaling pathway; cell proliferation; peptidyl-serine phosphorylation; protein import into nucleus, translocation; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; insulin receptor signaling pathway; positive regulation of vasoconstriction; innate immune response; gene expression; positive regulation of protein amino acid phosphorylation; blood coagulation; vascular endothelial growth factor receptor signaling pathway; phosphorylation; hyaluronan metabolic process

Disease: Schizophrenia; Cowden Syndrome 6; Proteus Syndrome; Breast Cancer; Ovarian Cancer
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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