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anti-AKT1/PKBa (pS473) antibody :: Rabbit AKT1/PKBa (pS473) Antibody

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Catalog # MBS462005
Unit / Price
  0.1 mg  /  $265 +1 FREE 8GB USB
Testing Data
Product Name

AKT1/PKBa (pS473), Antibody

Also Known As

Rabbit anti Phospho- AKT1/PKBalpha (pSER473) antibody

Product Synonym Names
Rabbit anti-AKT1 (pS473)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Specificity
The motif corresponding to the major autophosphorylation site of the human AKT1 kinase protein. This antibody does not cross react to the non-phosphorylated AKT1 or with other unrelated phosphorylated serine. Ser473 corresponds to QFSYS in human, rat, mouse, chicken and canis of AKT/PKB proteins
Purity/Purification
Site-directed adsorption and epitope affinity purification. This affinity purified antibody is supplied in sterile Tris-buffered saline (pH7.2) containing antibody stabilizer.
Concentration
Size: 100 ug/200 ul (lot specific)
Antigen Preparation
A synthetic peptide containing the phosphorylated Ser473 motif of human AKT1.
Positive Control
PDGF-Treated 3T3 cell lysate.
Cellular Location
Cell Cytoplasmic
Preparation and Storage
The antibodies are stable for 12 months from date of receipt when stored at -20 degree C to -70 degree C. The antibodies can be stored at 2 degree C-8 degree C for three month without detectable loss of activity. Avoid repeated freezing-thawing cycles.
Other Notes
Small volumes of anti-AKT1/PKBa (pS473) antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-AKT1/PKBa (pS473) antibody
Akt [also known as PKB (Protein kinase B) is a family of serine/threonine kinases that plays an important role in signal transduction. There are three known isoforms of Akt in mammalian cells [Akt1 (alpha), Akt2 (beta) and Akt3 (gamma)]; Akt is activated by insulin and growth and survival factors. Akt1 is phosphorylated within the activation loop at threonine 308 and the C-terminus at serine 473 (S473). Additionally, Akt has been referred to as an oncogene because it has increased activity in a number of tumors
Applications Tested/Suitable for anti-AKT1/PKBa (pS473) antibody
ELISA, Western Blot, Immunohistochemistry
Application Notes for anti-AKT1/PKBa (pS473) antibody
Western Blot: 0.1-0.2 ug/ml
ELISA: 0.01-0.1 ug/ml
Immunoprecipitation: 2-5 ug/ml
Immunohistochemistry: 1-2 ug/ml

Molecular Weight
~60 kDa
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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