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ALCAM clia kit :: Human Activated Leukocyte Cell Adhesion Molecule (ALCAM) CLIA Kit

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Catalog # MBS2025416 (SPECIAL PROMOTION: Get FREE Starbucks gift card ($5 for 48-Wells-Kit or $10 for 96-Wells-Kit. Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
Unit / Price
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Typical Testing Data/Standard Curve (for reference only)
Product Name

Activated Leukocyte Cell Adhesion Molecule (ALCAM), CLIA Kit

Full Product Name

Activated Leukocyte Cell Adhesion Molecule (ALCAM) CLIA Kit

Product Synonym Names
CD166; MEMD; Activated Leucocyte Cell Adhesion Molecule
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
OMIM
601662
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of Activated Leukocyte Cell Adhesion Molecule (ALCAM).
No significant cross-reactivity or interference between Activated Leukocyte Cell Adhesion Molecule (ALCAM) and analogues was observed.
Assay Type
Double-antibody Sandwich
Samples
Serum, Plasma, Tissue homogenates, Cell lysates, Cell culture supernates and Other Biological Fluids
Detection Range
27.4-20,000pg/mL
Sensitivity
< 12.9pg/mL
Application
Chemiluminescent immunoassay for Antigen Detection.
Intra-assay Precision (Precision within an assay)
3 samples with low, middle and high level Activated Leukocyte Cell Adhesion Molecule (ALCAM) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays)
3 samples with low, middle and high level Activated Leukocyte Cell Adhesion Molecule (ALCAM) were tested on 3 different plates, 8 replicates in each plate.
CV(%) =
SD/meanX100
Intra-Assay
CV<10%
Inter-Assay
CV<12%
Preparation and Storage
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
ISO Certification
Manufactured in an ISO 9001:2008 and ISO 13485:2003 Certified Laboratory.
Supply Chain Verification
Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customer’s specifications, please inquire.
Product Note
Our CLIA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of ALCAM clia kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
ALCAM clia kit
The microplate provided in this kit has been pre-coated with an antibody specific to Activated Leukocyte Cell Adhesion Molecule (ALCAM). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Activated Leukocyte Cell Adhesion Molecule (ALCAM). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Activated Leukocyte Cell Adhesion Molecule (ALCAM) level in the sample or standard.;

Typical Testing Data/Standard Curve (for reference only) of ALCAM clia kit
ALCAM clia kit Typical Testing Data/Standard Curve (for reference only) image
NCBI/Uniprot data below describe general gene information for ALCAM. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
33,878 Da
NCBI Official Full Name
Activated leukocyte cell adhesion molecule
NCBI Official Synonym Full Names
activated leukocyte cell adhesion molecule
NCBI Official Symbol
ALCAM  [Similar Products]
NCBI Official Synonym Symbols
MEMD; CD166
  [Similar Products]
NCBI Protein Information
CD166 antigen
UniProt Protein Name
CD166 antigen
UniProt Synonym Protein Names
Activated leukocyte cell adhesion molecule; CD_antigen: CD166
Protein Family
UniProt Gene Name
ALCAM  [Similar Products]
UniProt Synonym Gene Names
UniProt Entry Name
CD166_HUMAN
NCBI Summary for ALCAM
This gene encodes activated leukocyte cell adhesion molecule (ALCAM), also known as CD166 (cluster of differentiation 166), which is a member of a subfamily of immunoglobulin receptors with five immunoglobulin-like domains (VVC2C2C2) in the extracellular domain. This protein binds to T-cell differentiation antigene CD6, and is implicated in the processes of cell adhesion and migration. Multiple alternatively spliced transcript variants encoding different isoforms have been found. [provided by RefSeq, Aug 2011]
UniProt Comments for ALCAM
ALCAM: Cell adhesion molecule that binds to CD6. Involved in neurite extension by neurons via heterophilic and homophilic interactions. May play a role in the binding of T- and B-cells to activated leukocytes, as well as in interactions between cells of the nervous system. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Cell adhesion

Chromosomal Location of Human Ortholog: 3q13.1

Cellular Component: focal adhesion; cell soma; axon; integral to membrane; external side of plasma membrane

Molecular Function: protein binding; receptor binding

Biological Process: axon guidance; motor axon guidance; signal transduction; cell adhesion
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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