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ALCAM elisa kit :: Mouse activated leukocyte cell adhesion molecule ELISA Kit

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Catalog # MBS700580
Unit / Price
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  24-Strip-Wells (LIMIT 1)  /  $240 +1 FREE 8GB USB
  48-Strip-Wells  /  $435 +1 FREE 8GB USB
  96-Strip-Wells  /  $625 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,595 +3 FREE 8GB USB
  10x96-Strip-Wells  /  $4,905 +6 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

activated leukocyte cell adhesion molecule (ALCAM), ELISA Kit

Full Product Name

Mouse Activated Leukocyte Cell Adhesion Molecule, ALCAM ELISA Kit

Product Synonym Names
Mouse Activated Leukocyte Cell Adhesion Molecule; ALCAM ELISA Kit; CD166; FLJ38514; MEMD; MGC71733; activated leucocyte cell adhesion molecule; activated leukocyte cell adhesion molecule
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
MBS700580 Testing Data
3D Structure
ModBase 3D Structure for Q61490
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of mouse ALCAM. No significant cross-reactivity or interference between mouse ALCAM and analogues was observed.
Samples
Serum, plasma, tissue homogenates
Assay Type
Sandwich
Detection Range
1.25 ng/ml -80 ng/ml.
Sensitivity
The minimum detectable dose of mouse ALCAM is typically less than 0.31 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of ALCAM elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for ALCAMpurchase
MBS700580 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the activated leukocyte cell adhesion molecule (ALCAM) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing ALCAM. The ELISA analytical biochemical technique of the MBS700580 kit is based on ALCAM antibody-ALCAM antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ALCAM antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ALCAM. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
ALCAM elisa kit
Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for ALCAM has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ALCAM present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ALCAM is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ALCAM bound in the initial step. The color development is stopped and the intensity of the color is measured.

Typical Testing Data/Standard Curve (for reference only) of ALCAM elisa kit
ALCAM elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS700580. Click to request current manual
NCBI/Uniprot data below describe general gene information for ALCAM. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
65,092 Da
NCBI Official Full Name
CD166 antigen
NCBI Official Synonym Full Names
activated leukocyte cell adhesion molecule
NCBI Official Symbol
Alcam  [Similar Products]
NCBI Official Synonym Symbols
BEN; SC1; MuSC; CD166; AI853494; DM-GRASP
  [Similar Products]
NCBI Protein Information
CD166 antigen
UniProt Protein Name
CD166 antigen
UniProt Synonym Protein Names
Activated leukocyte cell adhesion molecule; Protein DM-GRASP; CD_antigen: CD166
Protein Family
UniProt Gene Name
Alcam  [Similar Products]
UniProt Entry Name
CD166_MOUSE
UniProt Comments for ALCAM
Function: Cell adhesion molecule that binds to CD6. Involved in neurite extension by neurons via heterophilic and homophilic interactions. May play a role in the binding of T- and B-cells to activated leukocytes, as well as in interactions between cells of the nervous system.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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