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ALDH1A3 elisa kit :: Guinea Pig Aldehyde dehydrogenase family 1 member A3 ELISA Kit

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Catalog # MBS731605
Unit / Price
  48-Strip-Wells  /  $440 +1 FREE 8GB USB
  96-Strip-Wells  /  $640 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,895 +3 FREE 8GB USB
  10x96-Strip-Wells  /  $5,415 +6 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

Aldehyde dehydrogenase family 1 member A3 (ALDH1A3), ELISA Kit

Popular Item
Full Product Name

Guinea pig Aldehyde dehydrogenase family 1 member A3 ELISA Kit

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
OMIM
gene 615113
Species Reactivity
Samples
Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type
Competitive
Detection Range
5-100ng/mL
Sensitivity
1.0ng/mL
Preparation and Storage
Store all reagents at 2-8 degree C.
Sample Preparation
We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of ALDH1A3 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forALDH1A3purchase
MBS731605 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Aldehyde dehydrogenase family 1 member A3 (ALDH1A3) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing ALDH1A3. The ELISA analytical biochemical technique of the MBS731605 kit is based on ALDH1A3 antibody-ALDH1A3 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ALDH1A3 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ALDH1A3. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
ALDH1A3 elisa kit
For Samples: Cell culture fluid, body fluid, tissue homogenate, serum and blood plasma

Intended Uses: This APN ELISA kit is intended for laboratory research use only and not for use in diagnostic or therapeutic procedures. The stop solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration of APN in the sample, this APN ELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versus APN concentration. The concentration of in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Principle of the Assay: The coated well immunoenzymatic assay for the quantitative measurement of APN utilizes a multiclonal anti-APN antibody and an APN-HRP conjugate. The assay sample and buffer are incubated together with APN-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the APN concentration since APN from samples and APN-HRP conjugate compete for the anti-APN antibody binding site. Since the number of sites is limited, as more sites are occupied by APN from the sample, fewer sites are left to bind APN-HRP conjugate. Standards of known APN concentrations are run concurrently with the samples being assayed and a standard curve is plotted relating the intensity of the color (Optical Density) to the concentration of APN. The APN concentration in each sample is interpolated from this standard curve.

Typical Testing Data/Standard Curve (for reference only) of ALDH1A3 elisa kit
ALDH1A3 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS731605. Click to request current manual
NCBI/Uniprot data below describe general gene information for ALDH1A3. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
56,108 Da
NCBI Official Full Name
aldehyde dehydrogenase family 1 member A3 isoform 1
NCBI Official Synonym Full Names
aldehyde dehydrogenase 1 family, member A3
NCBI Official Symbol
ALDH1A3  [Similar Products]
NCBI Official Synonym Symbols
ALDH6; MCOP8; RALDH3; ALDH1A6
  [Similar Products]
NCBI Protein Information
aldehyde dehydrogenase family 1 member A3; aldehyde dehydrogenase 6; acetaldehyde dehydrogenase 6; retinaldehyde dehydrogenase 3
UniProt Protein Name
Aldehyde dehydrogenase family 1 member A3
UniProt Synonym Protein Names
Aldehyde dehydrogenase 6; Retinaldehyde dehydrogenase 3; RALDH-3; RalDH3
UniProt Gene Name
ALDH1A3  [Similar Products]
UniProt Synonym Gene Names
ALDH6; RALDH-3; RalDH3  [Similar Products]
UniProt Entry Name
AL1A3_HUMAN
NCBI Summary for ALDH1A3
This gene encodes an aldehyde dehydrogenase enzyme that uses retinal as a substrate. Mutations in this gene have been associated with microphthalmia, isolated 8, and expression changes have also been detected in tumor cells. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2014]
UniProt Comments for ALDH1A3
ALDH1A3: Recognizes as substrates free retinal and cellular retinol-binding protein-bound retinal. Seems to be the key enzyme in the formation of an RA gradient along the dorso-ventral axis during the early eye development and also in the development of the olfactory system. Belongs to the aldehyde dehydrogenase family.

Protein type: Oxidoreductase; Carbohydrate Metabolism - glycolysis and gluconeogenesis; Amino Acid Metabolism - tyrosine; Amino Acid Metabolism - histidine; Xenobiotic Metabolism - drug metabolism - cytochrome P450; Amino Acid Metabolism - phenylalanine; EC 1.2.1.5; Xenobiotic Metabolism - metabolism by cytochrome P450

Chromosomal Location of Human Ortholog: 15q26.3

Cellular Component: cytoplasm; cytosol

Molecular Function: aldehyde dehydrogenase (NAD) activity; protein homodimerization activity; aldehyde dehydrogenase [NAD(P)+] activity; retinal dehydrogenase activity

Biological Process: inner ear morphogenesis; retinal metabolic process; retinol metabolic process; embryonic eye morphogenesis; positive regulation of apoptosis; righting reflex; olfactory pit development; optic cup morphogenesis involved in camera-type eye development; retinoic acid metabolic process; locomotory behavior; neuromuscular process controlling balance; nucleus accumbens development

Disease: Microphthalmia, Isolated 8
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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