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ALDOS elisa kit :: Rat Aldosterone Synthase (ALDOS) ELISA Kit

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Catalog # MBS2022150 (SPECIAL PROMOTION: Get FREE Starbucks gift card ($5 for 48-Wells-Kit or $10 for 96-Wells-Kit. Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
Unit / Price
  24-Strip-Wells  /  $265 +1 FREE 8GB USB
  48-Strip-Wells  /  $490 +1 FREE 8GB USB
  96-Strip-Wells  /  $655 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,660 +3 FREE 8GB USB
  10x96-Strip-Wells  /  $4,795 +5 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

Aldosterone Synthase (ALDOS), ELISA Kit

Also Known As

Aldosterone Synthase (ALDOS) ELISA Kit

Product Synonym Names
CPN2; CYP11B; CYP11BL; CYP11B2; P450C18; P450aldo; Steroid 18-hydroxylase; Aldosterone-synthesizing enzyme; Cytochrome P450 Family 11 Subfamily B Polypeptide 2
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
Sequence Length
500
3D Structure
ModBase 3D Structure for P15539
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of Aldosterone Synthase (ALDOS).
No significant cross-reactivity or interference between Aldosterone Synthase (ALDOS) and analogues was observed.
Assay Type
Double-antibody Sandwich
Samples
Serum, Plasma, Tissue homogenates and Other Biological Fluids
Detection Range
0.156-10ng/mL
Sensitivity
< 0.057ng/mL
Application
Enzyme-linked immunosorbent assay for Antigen Detection.
Intra-assay Precision (Precision within an assay)
3 samples with low, middle and high level Aldosterone Synthase (ALDOS) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays)
3 samples with low, middle and high level Aldosterone Synthase (ALDOS) were tested on 3 different plates, 8 replicates in each plate.
CV(%) =
SD/meanX100
Intra-Assay
CV<10%
Inter-Assay
CV<12%
Preparation and Storage
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of ALDOS elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forALDOSpurchase
MBS2022150 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Aldosterone Synthase (ALDOS) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing ALDOS. The ELISA analytical biochemical technique of the MBS2022150 kit is based on ALDOS antibody-ALDOS antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ALDOS antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ALDOS. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
ALDOS elisa kit
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Aldosterone Synthase (ALDOS). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Aldosterone Synthase (ALDOS). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Aldosterone Synthase (ALDOS), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Aldosterone Synthase (ALDOS) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Product Categories/Family for ALDOS elisa kit

Typical Testing Data/Standard Curve (for reference only) of ALDOS elisa kit
ALDOS elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS2022150. Click to request current manual
NCBI/Uniprot data below describe general gene information for ALDOS. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
57,373 Da
NCBI Official Full Name
aldosterone synthase
NCBI Official Synonym Full Names
cytochrome P450, family 11, subfamily b, polypeptide 2
NCBI Official Symbol
Cyp11b2  [Similar Products]
NCBI Official Synonym Symbols
Cpn2; Cyp11b; Cyp11b-2
  [Similar Products]
NCBI Protein Information
cytochrome P450 11B2, mitochondrial
UniProt Protein Name
Cytochrome P450 11B2, mitochondrial
UniProt Synonym Protein Names
Aldosterone synthase; CYPXIB2; Cytochrome P450C11; Steroid 11-beta-hydroxylase (EC:1.14.15.4, EC:1.14.15.5)
UniProt Gene Name
Cyp11b2  [Similar Products]
UniProt Synonym Gene Names
Cyp11b-2  [Similar Products]
UniProt Entry Name
C11B2_MOUSE
UniProt Comments for ALDOS
CYP11B2: Preferentially catalyzes the conversion of 11- deoxycorticosterone to aldosterone via corticosterone and 18- hydroxycorticosterone. Defects in CYP11B2 are the cause of corticosterone methyloxidase type 1 deficiency (CMO-1 deficiency); also known as aldosterone deficiency due to defect in 18- hydroxylase or aldosterone deficiency I. CMO-1 deficiency is an autosomal recessive disorder of aldosterone biosynthesis. There are two biochemically different forms of selective aldosterone deficiency be termed corticosterone methyloxidase (CMO) deficiency type 1 and type 2. In CMO-1 deficiency, aldosterone is undetectable in plasma, while its immediate precursor, 18- hydroxycorticosterone, is low or normal. Defects in CYP11B2 are the cause of corticosterone methyloxidase type 2 deficiency (CMO-2 deficiency). CMO-2 is an autosomal recessive disorder of aldosterone biosynthesis. In CMO-2 deficiency, aldosterone can be low or normal, but at the expense of increased secretion of 18- hydroxycorticosterone. Consequently, patients have a greatly increased ratio of 18-hydroxycorticosterone to aldosterone and a low ratio of corticosterone to 18-hydroxycorticosterone in serum. Defects in CYP11B2 are a cause of familial hyperaldosteronism type 1 (FH1). It is a disorder characterized by hypertension, variable hyperaldosteronism, and abnormal adrenal steroid production, including 18-oxocortisol and 18-hydroxycortisol. There is significant phenotypic heterogeneity, and some individuals never develop hypertension. The molecular defect causing hyperaldosteronism familial type 1 is an anti-Lepore-type fusion of the CYP11B1 and CYP11B2 genes. The hybrid gene has the promoting part of CYP11B1, ACTH-sensitive, and the coding part of CYP11B2. Belongs to the cytochrome P450 family.

Protein type: Lipid Metabolism - androgen and estrogen; EC 1.14.15.5; Mitochondrial; EC 1.14.15.4; Lipid Metabolism - C21-steroid hormone; Oxidoreductase

Cellular Component: membrane; mitochondrion; dendrite

Molecular Function: corticosterone 18-monooxygenase activity; oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen; steroid 11-beta-monooxygenase activity; iron ion binding; metal ion binding; heme binding; oxidoreductase activity; monooxygenase activity

Biological Process: regulation of systemic arterial blood pressure by circulatory renin-angiotensin; ion homeostasis; regulation of blood pressure; drinking behavior; C21-steroid hormone biosynthetic process; aldosterone mediated regulation of blood volume; glucocorticoid biosynthetic process; steroid biosynthetic process; aldosterone biosynthetic process; positive regulation of blood pressure
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Pathways associated with ALDOS elisa kit
 Products by Pathway  Pathway Diagram
 Biological Oxidations Pathway antibodies  Biological Oxidations Pathway Diagram
 C21-Steroid Hormone Biosynthesis, Progesterone => Corticosterone/aldosterone Pathway antibodies  C21-Steroid Hormone Biosynthesis, Progesterone => Corticosterone/aldosterone Pathway Diagram
 C21-Steroid Hormone Biosynthesis, Progesterone => Corticosterone/aldosterone Pathway antibodies  C21-Steroid Hormone Biosynthesis, Progesterone => Corticosterone/aldosterone Pathway Diagram
 C21-Steroid Hormone Biosynthesis, Progesterone => Cortisol/cortisone Pathway antibodies  C21-Steroid Hormone Biosynthesis, Progesterone => Cortisol/cortisone Pathway Diagram
 C21-Steroid Hormone Biosynthesis, Progesterone => Cortisol/cortisone Pathway antibodies  C21-Steroid Hormone Biosynthesis, Progesterone => Cortisol/cortisone Pathway Diagram
 Cytochrome P450 Pathway antibodies  Cytochrome P450 Pathway Diagram
 Cytochrome P450 - Arranged By Substrate Type Pathway antibodies  Cytochrome P450 - Arranged By Substrate Type Pathway Diagram
 Defective CYP11A1 Causes Adrenal Insufficiency, Congenital, With 46,XY Sex Reversal (AICSR) Pathway antibodies  Defective CYP11A1 Causes Adrenal Insufficiency, Congenital, With 46,XY Sex Reversal (AICSR) Pathway Diagram
 Defective CYP11B1 Causes Adrenal Hyperplasia 4 (AH4) Pathway antibodies  Defective CYP11B1 Causes Adrenal Hyperplasia 4 (AH4) Pathway Diagram
 Defective CYP11B2 Causes Corticosterone Methyloxidase 1 Deficiency (CMO-1 Deficiency) Pathway antibodies  Defective CYP11B2 Causes Corticosterone Methyloxidase 1 Deficiency (CMO-1 Deficiency) Pathway Diagram
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