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anti-ALK antibody :: anti-Human ALK Antibody

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Catalog # MBS854051
Unit / Price
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  0.1 mg  /  $215 +1 FREE 8GB USB
Product Name

ALK, Antibody

Full Product Name

ALK (Ab-1604) Antibody

Product Synonym Names
ALK tyrosine kinase receptor precursor; Anaplastic lymphoma kinase; CD246; EC 2.7.10.1; kinase ALK
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
OMIM
105590
3D Structure
ModBase 3D Structure for Q9UM73
Species Reactivity
Human
Specificity
ALK antibody detects endogenous levels of total ALK protein.
Purity/Purification
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form/Format
In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Immunogen
The antiserum was produced against synthesized peptide derived from Internal of human ALK.
Preparation and Storage
Store at-20 degree C for 1 year.
Other Notes
Small volumes of anti-ALK antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-ALK antibody
Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA (EIA)
Application Notes for anti-ALK antibody
IHC: 1:50-1:100
IF: 1:100-1:500
ELISA: 1:1000

Testing Data of anti-ALK antibody
Peptide-+
anti-ALK antibody Testing Data image
Immunohistochemistry (IHC) of anti-ALK antibody
Peptide-+ Immunohistochemistry analysis of paraffin-embedded human brain tissue using ALK antibody. Immunofluorescence analysis of HeLa cells, using ALK antibody.
anti-ALK antibody Immunohistochemistry (IHC) (IHC) image
NCBI/Uniprot data below describe general gene information for ALK. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
176,442 Da
NCBI Official Full Name
ALK tyrosine kinase receptor isoform 1
NCBI Official Synonym Full Names
ALK receptor tyrosine kinase
NCBI Official Symbol
NCBI Official Synonym Symbols
CD246; NBLST3
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NCBI Protein Information
ALK tyrosine kinase receptor
UniProt Protein Name
ALK tyrosine kinase receptor
UniProt Synonym Protein Names
Anaplastic lymphoma kinase; CD_antigen: CD246
Protein Family
UniProt Gene Name
NCBI Summary for ALK
This gene encodes a receptor tyrosine kinase, which belongs to the insulin receptor superfamily. This protein comprises an extracellular domain, an hydrophobic stretch corresponding to a single pass transmembrane region, and an intracellular kinase domain. It plays an important role in the development of the brain and exerts its effects on specific neurons in the nervous system. This gene has been found to be rearranged, mutated, or amplified in a series of tumours including anaplastic large cell lymphomas, neuroblastoma, and non-small cell lung cancer. The chromosomal rearrangements are the most common genetic alterations in this gene, which result in creation of multiple fusion genes in tumourigenesis, including ALK (chromosome 2)/EML4 (chromosome 2), ALK/RANBP2 (chromosome 2), ALK/ATIC (chromosome 2), ALK/TFG (chromosome 3), ALK/NPM1 (chromosome 5), ALK/SQSTM1 (chromosome 5), ALK/KIF5B (chromosome 10), ALK/CLTC (chromosome 17), ALK/TPM4 (chromosome 19), and ALK/MSN (chromosome X).[provided by RefSeq, Jan 2011]
UniProt Comments for ALK
Neuronal receptor tyrosine kinase that is essentially and transiently expressed in specific regions of the central and peripheral nervous systems and plays an important role in the genesis and differentiation of the nervous system. Transduces signals from ligands at the cell surface, through specific activation of the mitogen-activated protein kinase (MAPK) pathway. Phosphorylates almost exclusively at the first tyrosine of the Y-x-x-x-Y-Y motif. Following activation by ligand, ALK induces tyrosine phosphorylation of CBL, FRS2, IRS1 and SHC1, as well as of the MAP kinases MAPK1/ERK2 and MAPK3/ERK1. Acts as a receptor for ligands pleiotrophin (PTN), a secreted growth factor, and midkine (MDK), a PTN-related factor, thus participating in PTN and MDK signal transduction. PTN-binding induces MAPK pathway activation, which is important for the anti-apoptotic signaling of PTN and regulation of cell proliferation. MDK-binding induces phosphorylation of the ALK target insulin receptor substrate (IRS1), activates mitogen-activated protein kinases (MAPKs) and PI3-kinase, resulting also in cell proliferation induction. Drives NF-kappa-B activation, probably through IRS1 and the activation of the AKT serine/threonine kinase. Recruitment of IRS1 to activated ALK and the activation of NF-kappa-B are essential for the autocrine growth and survival signaling of MDK.
Product References and Citations for anti-ALK antibody
Brunangelo Falini, Blood, Nov 1999; 94: 3509-3515. Philipp B Staber, Blood, Aug 2007; 10.1182/blood-2007-02-071258. Monika Kasprzycka, PNAS, Jun 2006; 103: 9964-9969. Ritsuro Suzuki, Blood, Nov 2000; 96: 2993-3000.

Precautions
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Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

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