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ALOX12 sirna

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Catalog # MBS8207008
Unit / Price
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  15 nmol  /  $365 +1 FREE 8GB USB
  30 nmol  /  $515 +1 FREE 8GB USB
ALOX12 sirna
Product Name

ALOX12, siRNA

Full Product Name

ALOX12 siRNA (Human)

Product Synonym Names
12LO; LOG12; Arachidonate 12-lipoxygenase. 12S-type; 12S-LOX; 12S-lipoxygenase; Lipoxin synthase 12-LO; Platelet-type lipoxygenase 12
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
OMIM
114500
3D Structure
ModBase 3D Structure for P18054
Host
Synthetic
Species Reactivity
Human
Specificity
ALOX12 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human ALOX12 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of ALOX12 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
ALOX12 sirna
siRNA to inhibit ALOX12 expression using RNA interference
Applications Tested/Suitable for ALOX12 sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for ALOX12. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
75,694 Da
NCBI Official Full Name
arachidonate 12-lipoxygenase, 12S-type
NCBI Official Synonym Full Names
arachidonate 12-lipoxygenase
NCBI Official Symbol
ALOX12  [Similar Products]
NCBI Official Synonym Symbols
LOG12; 12-LOX; 12S-LOX
  [Similar Products]
NCBI Protein Information
arachidonate 12-lipoxygenase, 12S-type
UniProt Protein Name
Arachidonate 12-lipoxygenase, 12S-type
UniProt Synonym Protein Names
Lipoxin synthase 12-LO (EC:3.3.2.-); Platelet-type lipoxygenase 12
UniProt Gene Name
ALOX12  [Similar Products]
UniProt Synonym Gene Names
12LO; LOG12; 12S-LOX; 12S-lipoxygenase  [Similar Products]
UniProt Entry Name
LOX12_HUMAN
UniProt Comments for ALOX12
ALOX12: Oxygenase and 14,15-leukotriene A4 synthase activity. Belongs to the lipoxygenase family.

Protein type: Apoptosis; Oxidoreductase; Lipid Metabolism - arachidonic acid; EC 1.13.11.31

Chromosomal Location of Human Ortholog: 17p13.1

Cellular Component: membrane; cytoplasm; cytosol; sarcolemma

Molecular Function: oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen; arachidonate 12-lipoxygenase activity; hepoxilin A3 synthase activity; protein binding; hepoxilin-epoxide hydrolase activity; lipoxygenase activity; iron ion binding

Biological Process: positive regulation of cell adhesion; positive regulation of smooth muscle cell proliferation; linoleic acid metabolic process; positive regulation of caspase activity; positive regulation of vasodilation; positive regulation of cell growth; positive regulation of angiogenesis; positive regulation of endothelial cell differentiation; lipoxygenase pathway; positive regulation of mitochondrial depolarization; positive regulation of cell proliferation; arachidonic acid metabolic process; fatty acid oxidation; hepoxilin biosynthetic process; cell motility; superoxide release; hepoxilin metabolic process; positive regulation of cell migration; aging; negative regulation of apoptosis
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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