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ALOX15B elisa kit :: Human Arachidonate 15-lipoxygenase B ELISA Kit

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Catalog # MBS760926
Unit / Price
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  48-Strip-Wells  /  $300 +1 FREE 8GB USB
  96-Strip-Wells  /  $415 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $1,825 +2 FREE 8GB USB
  10x96-Strip-Wells  /  $3,425 +4 FREE 8GB USB
Product Name

Arachidonate 15-lipoxygenase B (ALOX15B), ELISA Kit

Popular Item
Full Product Name

Human Arachidonate 15-lipoxygenase B ELISA Kit

Product Synonym Names
ALOX15B (Arachidonate 15-lipoxygenase B)/15-LOX-B/15-LOX-2/Linoleate 13-lipoxygenase 15-Lob/15-lipoxygenase 2/Arachidonate 15-lipoxygenase type II
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
Species Reactivity
Samples
Serum, plasma and other biological fluids.
Assay Type
Sandwich ELISA, Double Antibody
Detection Range
0.312-20ng/ml
Sensitivity
0.188ng/ml
Preparation and Storage
Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of ALOX15B elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for ALOX15Bpurchase
MBS760926 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Arachidonate 15-lipoxygenase B (ALOX15B) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing ALOX15B. The ELISA analytical biochemical technique of the MBS760926 kit is based on ALOX15B antibody-ALOX15B antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ALOX15B antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ALOX15B. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
ALOX15B elisa kit
Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Human ALOX15B antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human ALOX15B antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human ALOX15B amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human ALOX15B can be calculated.

Typical Testing Data of ALOX15B elisa kit
ALOX15B elisa kit Typical Testing Data image
Typical Standard Curve of ALOX15B elisa kit
ALOX15B elisa kit Typical Standard Curve image
NCBI/Uniprot data below describe general gene information for ALOX15B. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Related Accession #
Molecular Weight
76,145 Da
NCBI Official Full Name
arachidonate 15-lipoxygenase B
NCBI Official Synonym Full Names
arachidonate 15-lipoxygenase, type B
NCBI Official Symbol
Alox15b  [Similar Products]
NCBI Protein Information
arachidonate 15-lipoxygenase B
UniProt Protein Name
Arachidonate 15-lipoxygenase B
UniProt Synonym Protein Names
15-lipoxygenase 2; 15-LOX-2; Arachidonate 15-lipoxygenase type II; Linoleate 13-lipoxygenase 15-LOb (EC:1.13.11.-)
UniProt Gene Name
Alox15b  [Similar Products]
UniProt Synonym Gene Names
15-LOX-B; 15-LOX-2  [Similar Products]
UniProt Entry Name
LX15B_RAT
NCBI Summary for ALOX15B
human homolog is an epidermis-type lipoxygenase and member of the multigene family of mammalian lipoxygenases [RGD, Feb 2006]
UniProt Comments for ALOX15B
ALOX15B: Converts arachidonic acid exclusively to 15S- hydroperoxyeicosatetraenoic acid, while linoleic acid is less well metabolized. Belongs to the lipoxygenase family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Apoptosis; Cell cycle regulation; Oxidoreductase; Lipid Metabolism - arachidonic acid; EC 1.13.11.33; Motility/polarity/chemotaxis

Cellular Component: cytoplasm; cytoskeleton; cytosol; extrinsic to membrane; intracellular; membrane; nucleus; plasma membrane

Molecular Function: arachidonate 15-lipoxygenase activity; calcium ion binding; iron ion binding; lipid binding; lipoxygenase activity

Biological Process: arachidonic acid metabolic process; hepoxilin biosynthetic process; linoleic acid metabolic process; lipoxygenase pathway; negative regulation of cell cycle; negative regulation of cell proliferation; negative regulation of growth; positive regulation of keratinocyte differentiation
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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