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alpha1-AGP elisa kit :: Human alpha1-AGP ELISA Kit

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Catalog # MBS2511738
Unit / Price
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Typical Testing Data/Standard Curve (for reference only)
Product Name

alpha1-AGP, ELISA Kit

Popular Item
Full Product Name

Human alpha1-AGP (Alpha-1-Acid Glycoprotein) ELISA Kit

Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
OMIM
138610
3D Structure
ModBase 3D Structure for P19652
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of Human alpha1-AGP. No significant cross-reactivity or interference between Human alpha1-AGP and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Human alpha1-AGP and all the analogues, therefore, cross reaction may still exist.
Samples
Serum, Plasma, Biological Fluids
Assay Type
Sandwich
Detection Range
3.125-200ng/mL
Sensitivity
Min: 1.875ng/mL; Max: 200ng/mL
Preparation and Storage
Store at 4 degree C.
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Product Note
Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of alpha1-AGP elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for alpha1-AGPpurchase
MBS2511738 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the alpha1-AGP, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing alpha1-AGP. The ELISA analytical biochemical technique of the MBS2511738 kit is based on alpha1-AGP antibody-alpha1-AGP antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect alpha1-AGP antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, alpha1-AGP. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
alpha1-AGP elisa kit
Description: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of alpha1-AGP in human serum, plasma and other biological fluids
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human alpha1-AGP. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Human alpha1-AGP and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human alpha1-AGP, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human alpha1-AGP. You can calculate the concentration of Human alpha1-AGP in the samples by comparing the OD of the samples to the standard curve.

Typical Testing Data/Standard Curve (for reference only) of alpha1-AGP elisa kit
alpha1-AGP elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS2511738. Click to request current manual
NCBI/Uniprot data below describe general gene information for alpha1-AGP. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
23,603 Da
NCBI Official Full Name
alpha-1-acid glycoprotein 2
NCBI Official Synonym Full Names
orosomucoid 2
NCBI Official Symbol
NCBI Official Synonym Symbols
AGP2; AGP-B; AGP-B'
  [Similar Products]
NCBI Protein Information
alpha-1-acid glycoprotein 2; AGP 2; OMD 2; alpha-1-acid glycoprotein, type 2; orosomucoid-2
UniProt Protein Name
Alpha-1-acid glycoprotein 2
UniProt Synonym Protein Names
Orosomucoid-2; OMD 2
UniProt Gene Name
UniProt Synonym Gene Names
AGP2; AGP 2; OMD 2  [Similar Products]
UniProt Entry Name
A1AG2_HUMAN
NCBI Summary for alpha1-AGP
This gene encodes a key acute phase plasma protein. Because of its increase due to acute inflammation, this protein is classified as an acute-phase reactant. The specific function of this protein has not yet been determined; however, it may be involved in aspects of immunosuppression. [provided by RefSeq, Jul 2008]
UniProt Comments for alpha1-AGP
ORM2: Functions as transport protein in the blood stream. Binds various hydrophobic ligands in the interior of its beta- barrel domain. Also binds synthetic drugs and influences their distribution and availability. Appears to function in modulating the activity of the immune system during the acute-phase reaction. Belongs to the calycin superfamily. Lipocalin family.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 9q32

Cellular Component: extracellular space

Biological Process: regulation of immune system process; transport; acute-phase response
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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