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alpha2-AP elisa kit :: Rat alpha2-Antiplasmin (alpha2-AP) ELISA Kit

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Catalog # MBS262375
Unit / Price
  48-Strip-Wells  /  $275 +1 FREE 8GB USB
  96-Strip-Wells  /  $435 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $1,755 +2 FREE 8GB USB
  10x96-Strip-Wells  /  $3,150 +3 FREE 8GB USB
Typical Standard Curve/Testing Data
Product Name

alpha2-Antiplasmin (alpha2-AP), ELISA Kit

Popular Item
Also Known As

Rat alpha2-Antiplasmin (alpha2-AP) ELISA Kit

Product Synonym Names
Rat a2-Antiplasmin (a2-AP) ELISA Kit
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
Sequence Length
491
OMIM
613168
Species Reactivity
Specificity
No cross-reaction with other factors.
Samples
Serum, plasma or cell culture supernatant
Assay Type
Quantitative Sandwich
Detection Range
20 ng/ml-0.312 ng/ml
Sensitivity
Up to 0.06 ng/ml.
Intra-assay Precision
<= 8%
Inter-assay Precision
<= 12%
Preparation and Storage
Store all reagents at 2-8 degree C.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of alpha2-AP elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms foralpha2-APpurchase
MBS262375 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the alpha2-Antiplasmin (alpha2-AP) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing alpha2-AP. The ELISA analytical biochemical technique of the MBS262375 kit is based on alpha2-AP antibody-alpha2-AP antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect alpha2-AP antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, alpha2-AP. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
alpha2-AP elisa kit
Principle of the Assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Rat alpha2-AP monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

Typical Standard Curve/Testing Data of alpha2-AP elisa kit
alpha2-AP elisa kit Typical Standard Curve/Testing Data image
Sample Manual Insert of MBS262375. Click to request current manual
NCBI/Uniprot data below describe general gene information for alpha2-AP. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
47,907 Da
NCBI Official Full Name
alpha-2-antiplasmin isoform a
NCBI Official Synonym Full Names
serpin peptidase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 2
NCBI Official Symbol
SERPINF2  [Similar Products]
NCBI Official Synonym Symbols
AAP; API; PLI; A2AP; ALPHA-2-PI
  [Similar Products]
NCBI Protein Information
alpha-2-antiplasmin; serpin F2; alpha-2-AP; alpha-2-plasmin inhibitor; serine (or cysteine) proteinase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 2
UniProt Protein Name
Alpha-2-antiplasmin
UniProt Synonym Protein Names
Alpha-2-plasmin inhibitor; Alpha-2-PI; Serpin F2
UniProt Gene Name
SERPINF2  [Similar Products]
UniProt Synonym Gene Names
AAP; PLI; Alpha-2-AP; Alpha-2-PI  [Similar Products]
UniProt Entry Name
A2AP_HUMAN
NCBI Summary for alpha2-AP
This gene encodes a member of the serpin family of serine protease inhibitors. The protein is a major inhibitor of plasmin, which degrades fibrin and various other proteins. Consequently, the proper function of this gene has a major role in regulating the blood clotting pathway. Mutations in this gene result in alpha-2-plasmin inhibitor deficiency, which is characterized by severe hemorrhagic diathesis. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2009]
UniProt Comments for alpha2-AP
SERPINF2: Serine protease inhibitor. The major targets of this inhibitor are plasmin and trypsin, but it also inactivates matriptase-3/TMPRSS7 and chymotrypsin. Defects in SERPINF2 are the cause of alpha-2-plasmin inhibitor deficiency (APLID). APLID is an autosomal recessive disorder resulting in severe hemorrhagic diathesis. Belongs to the serpin family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 17p13

Cellular Component: extracellular space; cell surface; fibrinogen complex; extracellular region

Molecular Function: serine-type endopeptidase inhibitor activity; protein binding; protein homodimerization activity; protease binding; endopeptidase inhibitor activity

Biological Process: platelet activation; collagen fibril organization; positive regulation of smooth muscle cell proliferation; positive regulation of collagen biosynthetic process; positive regulation of JNK cascade; response to organic substance; fibrinolysis; negative regulation of fibrinolysis; platelet degranulation; renin-angiotensin regulation of blood vessel size; positive regulation of stress fiber formation; acute-phase response; blood vessel morphogenesis; positive regulation of transcription from RNA polymerase II promoter; positive regulation of cell differentiation; blood coagulation

Disease: Alpha-2-plasmin Inhibitor Deficiency
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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