• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

ALT elisa kit :: Mouse Alanine Aminotransferase (ALT) ELISA Kit

Scan QR to view Datasheet
Catalog # MBS2701479 (SPECIAL PROMOTIONAL PRICING for a limited time)
Unit / Price
Scan QR to view Datasheet
  24-Strip-Wells  /  $210 +1 FREE 8GB USB
  48-Strip-Wells  /  $340 +1 FREE 8GB USB
  96-Strip-Wells  /  $450 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $1,845 +2 FREE 8GB USB
  10x96-Strip-Wells  /  $3,430 +4 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

Alanine Aminotransferase (ALT), ELISA Kit

Popular Item
Full Product Name

Mouse Alanine Aminotransferase (ALT) ELISA Kit

Product Synonym Names
GPT; ALAT; SGPT; AAT1; GPT1; Serum Glutamic Pyruvic Transaminase; Alanine Transaminase; Glutamate pyruvate transaminase 1; Glutamic--alanine transaminase 1
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
OMIM
U70732 Genomic DNA
3D Structure
ModBase 3D Structure for P24298
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of Alanine Aminotransferase (ALT).
No significant cross-reactivity or interference between Alanine Aminotransferase (ALT) and analogues was observed.
Assay Type
Double-antibody Sandwich
Samples
Serum, Plasma, Tissue Homogenates, Cell Lysates and Other Biological Fluids
Detection Range
0.156-10ng/mL
Sensitivity
< 0.064ng/mL
Application
Enzyme-linked immunosorbent assay for Antigen Detection.
Intra-assay Precision (Precision within an assay)
3 samples with low, middle and high level Alanine Aminotransferase (ALT) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays)
3 samples with low, middle and high level Alanine Aminotransferase (ALT) were tested on 3 different plates, 8 replicates in each plate.
CV(%) =
SD/meanX100
Intra-Assay
CV<10%
Inter-Assay
CV<12%
Preparation and Storage
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
ISO Certification
Manufactured in an ISO 9001:2008 and ISO 13485:2003 Certified Laboratory.
Supply Chain Verification
Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customer’s specifications, please inquire.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of ALT elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for ALTpurchase
MBS2701479 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Alanine Aminotransferase (ALT) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing ALT. The ELISA analytical biochemical technique of the MBS2701479 kit is based on ALT antibody-ALT antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ALT antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ALT. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
ALT elisa kit
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Alanine Aminotransferase (ALT). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Alanine Aminotransferase (ALT). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Alanine Aminotransferase (ALT), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Alanine Aminotransferase (ALT) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Typical Testing Data/Standard Curve (for reference only) of ALT elisa kit
ALT elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS2701479. Click to request current manual
NCBI/Uniprot data below describe general gene information for ALT. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
54,637 Da
NCBI Official Full Name
alanine aminotransferase, partial
NCBI Official Synonym Full Names
glutamic--pyruvic transaminase
NCBI Official Symbol
NCBI Official Synonym Symbols
AAT1; ALT1; GPT1
  [Similar Products]
NCBI Protein Information
alanine aminotransferase 1
UniProt Protein Name
Alanine aminotransferase 1
UniProt Synonym Protein Names
Glutamate pyruvate transaminase 1; GPT 1; Glutamic--alanine transaminase 1; Glutamic--pyruvic transaminase 1
UniProt Gene Name
UniProt Synonym Gene Names
AAT1; GPT1; ALT1; GPT 1  [Similar Products]
NCBI Summary for ALT
This gene encodes cytosolic alanine aminotransaminase 1 (ALT1); also known as glutamate-pyruvate transaminase 1. This enzyme catalyzes the reversible transamination between alanine and 2-oxoglutarate to generate pyruvate and glutamate and, therefore, plays a key role in the intermediary metabolism of glucose and amino acids. Serum activity levels of this enzyme are routinely used as a biomarker of liver injury caused by drug toxicity, infection, alcohol, and steatosis. A related gene on chromosome 16 encodes a putative mitochondrial alanine aminotransaminase.[provided by RefSeq, Nov 2009]
UniProt Comments for ALT
Catalyzes the reversible transamination between alanine and 2-oxoglutarate to form pyruvate and glutamate. Participates in cellular nitrogen metabolism and also in liver gluconeogenesis starting with precursors transported from skeletal muscles ().
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Products associated with ALT elisa kit
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000