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AMPD2 elisa kit :: Mouse adenosine monophosphate deaminase 2 (isoform L) ELISA Kit

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Catalog # MBS913798
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Typical Testing Data/Standard Curve (for reference only)
Product Name

adenosine monophosphate deaminase 2 (isoform L) (AMPD2), ELISA Kit

Popular Item
Also Known As

Mouse AMP deaminase 2, AMPD2 ELISA Kit

Product Synonym Names
Mouse AMP deaminase 2 (AMPD2) ELISA kit; AMP deaminase 2; OTTHUMP00000013371; adenosine monophosphate deaminase 2 (isoform L)
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
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MBS913798 Testing Data
Sequence Length
798
3D Structure
ModBase 3D Structure for Q9DBT5
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of mouse AMPD2. No significant cross-reactivity or interference between mouse AMPD2 and analogues was observed.
Samples
Serum, plasma, tissue homogenates, Cell lysates
Assay Type
Sandwich
Detection Range
28 pg/ml -1800 pg/ml.
Sensitivity
The minimum detectable dose of mouse AMPD2 is typically less than 7 pg/ml.The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of AMPD2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forAMPD2purchase
MBS913798 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the adenosine monophosphate deaminase 2 (isoform L) (AMPD2) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing AMPD2. The ELISA analytical biochemical technique of the MBS913798 kit is based on AMPD2 antibody-AMPD2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect AMPD2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, AMPD2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
AMPD2 elisa kit
Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for AMPD2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any AMPD2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for AMPD2 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of AMPD2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Typical Testing Data/Standard Curve (for reference only) of AMPD2 elisa kit
AMPD2 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS913798. Click to request current manual
NCBI/Uniprot data below describe general gene information for AMPD2. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
92,024 Da
NCBI Official Full Name
AMP deaminase 2 isoform b
NCBI Official Synonym Full Names
adenosine monophosphate deaminase 2
NCBI Official Symbol
Ampd2  [Similar Products]
NCBI Official Synonym Symbols
Ampd-2; AI552571; 1200014F01Rik
  [Similar Products]
NCBI Protein Information
AMP deaminase 2; adenosine monophosphate deaminase 2 (isoform L)
UniProt Protein Name
AMP deaminase 2
UniProt Synonym Protein Names
AMP deaminase isoform L
Protein Family
UniProt Gene Name
Ampd2  [Similar Products]
UniProt Entry Name
AMPD2_MOUSE
UniProt Comments for AMPD2
AMPD2: AMP deaminase plays a critical role in energy metabolism. Belongs to the adenosine and AMP deaminases family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Nucleotide Metabolism - purine; Hydrolase; EC 3.5.4.6

Molecular Function: hydrolase activity; metal ion binding; deaminase activity; AMP deaminase activity

Biological Process: IMP biosynthetic process; purine ribonucleoside monophosphate biosynthetic process; IMP salvage; nucleotide metabolic process
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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