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ANA elisa kit :: Mouse anti-nuclear ELISA Kit

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Catalog # MBS282118
Unit / Price
  48-Strip-Wells  /  $405 +1 FREE 8GB USB
  96-Strip-Wells  /  $675 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $3,080 +3 FREE 8GB USB
  10x96-Strip-Wells  /  $5,805 +7 FREE 8GB USB
ANA elisa kit
Product Name

anti-nuclear (ANA), ELISA Kit

Popular Item
Also Known As

Mouse anti-nuclear Antibody (IgG)ELISA Kit

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of Mouse ANA-IgG. No significant cross-reactivity or interference between Mouse ANA-IgG and analogues was observed.
Samples
serum, plasma and other biological fluids
Detection Range
3.12 pg/mL - 200 pg/mL
Sensitivity
1.04 pg/mL (mean of 6 independent assays).
Intra-assay Precision
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision. Intra-Assay: CV<8%
Inter-assay Precision
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in forty separate assays to assess inter-assay precision. CV (%) = SD/meanX100. Inter-Assay: CV<12%
Preparation and Storage
Store at 2-8 degree C.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of ANA elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forANApurchase
MBS282118 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the anti-nuclear (ANA) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing ANA. The ELISA analytical biochemical technique of the MBS282118 kit is based on ANA antibody-ANA antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ANA antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ANA. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
ANA elisa kit
Intended Uses: For the quantitative detection of Mouse Anti-nuclear IgG antibody (ANA-IgG) concentration in serum, plasma and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with anti-mouse IgG antibody. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antigen and Streptavidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a substrate solution is added to each well. Only those wells that contain anti-mouse IgG antibody, biotin-conjugated antigen and enzyme-conjugated Streptavidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of ANA-IgG in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Sample Manual Insert of MBS282118. Click to request current manual
Protein Family
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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