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ANA elisa kit :: Mouse Anti-nuclear ELISA Kit

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Catalog # MBS733339
Unit / Price
  48-Strip-Wells  /  $440 +1 FREE 8GB USB
  96-Strip-Wells  /  $640 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,895 +3 FREE 8GB USB
  10x96-Strip-Wells  /  $5,415 +6 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

Anti-nuclear (ANA), ELISA Kit

Popular Item
Full Product Name

Mouse Anti-nuclear Antibody ELISA Kit

Product Synonym Names
Mouse Anti-nuclear Antibody ELISA Kit; Anti-nuclear; Anti-nuclear Antibody (Mouse)
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
Chromosome Location
Chromosome: 21; NC_000021.8 (18965968..18985268, complement). Location: 21q21.1
OMIM
605674
3D Structure
ModBase 3D Structure for Q14201
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of ANAb. No significant cross-reactivity or interference between ANAb and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between ANAb and all the analogues, therefore, cross reaction may still exist in some cases.
Samples
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Type
Competitive (Quantitative)
Sensitivity
0.1 ng/mL.
Preparation and Storage
Store all reagents at 2-8 degree C
Sample Preparation
We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of ANA elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forANApurchase
MBS733339 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Anti-nuclear (ANA) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing ANA. The ELISA analytical biochemical technique of the MBS733339 kit is based on ANA antibody-ANA antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ANA antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ANA. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
ANA elisa kit
Intended Uses: This ANAb ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse ANAb. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Principle of the Assay: ANAb ELISA kit applies the competitive enzyme immunoassay technique utilizing Nuclear antigen and an ANAb-HRP conjugate. The assay sample and buffer are incubated together with ANAb-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ANAb concentration since ANAb from samples and ANAb-HRP conjugate compete for the Nuclear antigen binding site. Since the number of sites is limited, as more sites are occupied by ANAb from the sample, fewer sites are left to bind ANAb-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ANAb concentration in each sample is interpolated from this standard curve.
Product Categories/Family for ANA elisa kit

Typical Testing Data/Standard Curve (for reference only) of ANA elisa kit
ANA elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS733339. Click to request current manual
NCBI/Uniprot data below describe general gene information for ANA. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
29,116 Da
NCBI Official Full Name
ANA
NCBI Official Synonym Full Names
BTG family, member 3
NCBI Official Symbol
NCBI Official Synonym Symbols
ANA; TOB5; TOFA; TOB55; MGC8928
  [Similar Products]
NCBI Protein Information
protein BTG3; protein Tob5; OTTHUMP00000083229; OTTHUMP00000083230; B-cell translocation gene 3; abundant in neuroepithelium area protein
UniProt Protein Name
Protein BTG3
UniProt Synonym Protein Names
Abundant in neuroepithelium area protein; BTG family member 3; Protein Tob5
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
ANA; TOB5  [Similar Products]
UniProt Entry Name
BTG3_HUMAN
NCBI Summary for ANA
The protein encoded by this gene is a member of the BTG/Tob family. This family has structurally related proteins that appear to have antiproliferative properties. This encoded protein might play a role in neurogenesis in the central nervous system. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]
UniProt Comments for ANA
BTG3: Overexpression impairs serum-induced cell cycle progression from the G0/G1 to S phase. Belongs to the BTG family. 2 isoforms of the human protein are produced by alternative splicing.

Chromosomal Location of Human Ortholog: 21q21.1

Cellular Component: cytoplasm

Molecular Function: protein binding

Biological Process: negative regulation of cell proliferation; negative regulation of mitotic cell cycle
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Products associated withANA elisa kit
 Reference Product  PubMed Publications
 CNOT7 elisa kit  >2 publications with ANA and CNOT7
Pathways associated with ANA elisa kit
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