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ANF elisa kit :: Mouse atrial natriuretic factor ELISA Kit

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Catalog # MBS731491
Unit / Price
  48-Strip-Wells  /  $440 +1 FREE 8GB USB
  96-Strip-Wells  /  $640 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,895 +3 FREE 8GB USB
  10x96-Strip-Wells  /  $5,415 +6 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

atrial natriuretic factor (ANF), ELISA Kit

Popular Item
Full Product Name

Mouse atrial natriuretic factor ELISA Kit

Product Synonym Names
Mouse atrial natriuretic factor ELISA Kit; atrial natriuretic factor; atrial natriuretic factor (Mouse)
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
3D Structure
ModBase 3D Structure for P07500
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of ANP. No significant cross-reactivity or interference between ANP and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between ANP and all the analogues, therefore, cross reaction may still exist in some cases.
Samples
Serum, plasma, cell culture superna tants, body fluid and tissue homogenate
Assay Type
Competitive
Sensitivity
1.0 pg/mL.
Preparation and Storage
Store all reagents at 2-8 degree C
Sample Preparation
We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of ANF elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forANFpurchase
MBS731491 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the atrial natriuretic factor (ANF) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing ANF. The ELISA analytical biochemical technique of the MBS731491 kit is based on ANF antibody-ANF antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ANF antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ANF. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
ANF elisa kit
For samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

INTENDED USE This ANP ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse ANP. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

PRINCIPLE OF THE ASSAY ANP ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ANP antibody and an ANP-HRP conjugate. The assay sample and buffer are incubated together with ANP-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ANP concentration since ANP from samples and ANP-HRP conjugate compete for the anti-ANP antibody binding site. Since the number of sites is limited ,as more sites are occupied by ANP from the sample, fewer sites are left to bind ANP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ANP concentration in each sample is interpolated from this standard curve.
Product Categories/Family for ANF elisa kit

Typical Testing Data/Standard Curve (for reference only) of ANF elisa kit
ANF elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS731491. Click to request current manual
NCBI/Uniprot data below describe general gene information for ANF. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Related Accession #
Molecular Weight
16,843 Da
NCBI Official Full Name
atrial natriuretic factor
NCBI Official Symbol
NCBI Protein Information
natriuretic peptides A; ANF; ANP; prepronatriodilatin; atrial natriuretic factor; atrial natriuretic peptide; natriuretic peptide precursor A
UniProt Protein Name
Natriuretic peptides A
UniProt Synonym Protein Names
PrepronatriodilatinCleaved into the following chain:Atrial natriuretic factor; ANF; Alternative name(s):; Atrial natriuretic peptide
UniProt Gene Name
UniProt Entry Name
ANF_RABIT
UniProt Comments for ANF
Function: Hormone playing a key role in cardiovascular homeostasis through regulation of natriuresis, diuresis, and vasodilatation. Specifically binds and stimulates the cGMP production of the NPR1 receptor. Binds the clearance receptor NPR3

Subcellular location: Secreted.

Post-translational modification: Cleaved by CORIN upon secretion to produce the functional hormone

Sequence similarities: Belongs to the natriuretic peptide family.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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