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ANG elisa kit :: Human ANG ELISA Kit

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Catalog # MBS2514487 (SPECIAL PROMOTIONAL PRICING for a limited time)
Unit / Price
  48-Strip-Wells  /  $310 +1 FREE 8GB USB
  96-Strip-Wells  /  $355 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $1,435 +1 FREE 8GB USB
  10x96-Strip-Wells  /  $2,805 +3 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

ANG, ELISA Kit

Popular Item
Also Known As

Human ANG (Angiogenin) ELISA Kit

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
Sequence Length
147
OMIM
105850
3D Structure
ModBase 3D Structure for P03950
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of Human ANG. No significant cross-reactivity or interference between Human ANG and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Human ANG and all the analogues, therefore, cross reaction may still exist.
Samples
Serum, Plasma, Biological Fluids
Assay Type
Sandwich
Detection Range
15.625-1000pg/mL
Sensitivity
Min: 9.375pg/mL; Max: 1000pg/mL
Preparation and Storage
Store at 4 degree C.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of ANG elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forANGpurchase
MBS2514487 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the ANG, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing ANG. The ELISA analytical biochemical technique of the MBS2514487 kit is based on ANG antibody-ANG antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ANG antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ANG. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
ANG elisa kit
Description: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of ANG in human serum, plasma and other biological fluids
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human ANG. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Human ANG and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human ANG, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human ANG. You can calculate the concentration of Human ANG in the samples by comparing the OD of the samples to the standard curve.

Typical Testing Data/Standard Curve (for reference only) of ANG elisa kit
ANG elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS2514487. Click to request current manual
NCBI/Uniprot data below describe general gene information for ANG. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
16,550 Da
NCBI Official Full Name
angiogenin
NCBI Official Synonym Full Names
angiogenin, ribonuclease, RNase A family, 5
NCBI Official Symbol
NCBI Official Synonym Symbols
ALS9; RAA1; HEL168; RNASE4; RNASE5
  [Similar Products]
NCBI Protein Information
angiogenin; RNase 5; epididymis luminal protein 168; ribonuclease 5; ribonuclease A A1
UniProt Protein Name
Angiogenin
UniProt Synonym Protein Names
Ribonuclease 5; RNase 5
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
RNASE5; RNase 5  [Similar Products]
UniProt Entry Name
ANGI_HUMAN
NCBI Summary for ANG
The protein encoded by this gene is an exceedingly potent mediator of new blood vessel formation. It hydrolyzes cellular tRNAs resulting in decreased protein synthesis and is similar to pancreatic ribonuclease. In addition, the mature peptide has antimicrobial activity against some bacteria and fungi, including S. pneumoniae and C. albicans. Alternative splicing results in two transcript variants encoding the same protein. This gene and the gene that encodes ribonuclease, RNase A family, 4 share promoters and 5' exons. Each gene splices to a unique downstream exon that contains its complete coding region. [provided by RefSeq, Aug 2014]
UniProt Comments for ANG
ANG: May function as a tRNA-specific ribonuclease that abolishes protein synthesis by specifically hydrolyzing cellular tRNAs. Binds to actin on the surface of endothelial cells; once bound, angiogenin is endocytosed and translocated to the nucleus. Angiogenin induces vascularization of normal and malignant tissues. Angiogenic activity is regulated by interaction with RNH1 in vivo. Defects in ANG are the cause of susceptibility to amyotrophic lateral sclerosis type 9 (ALS9). ALS is a degenerative disorder of motor neurons in the cortex, brain stem and spinal cord. ALS is characterized by muscular weakness and atrophy. Belongs to the pancreatic ribonuclease family.

Protein type: Nucleolus; Extracellular matrix; Motility/polarity/chemotaxis; Cell cycle regulation; Secreted, signal peptide; Ribonuclease; RNA-binding; EC 3.1.27.-; Secreted

Chromosomal Location of Human Ortholog: 14q11.1-q11.2

Cellular Component: angiogenin-PRI complex; extracellular space; growth cone; cell soma; nucleolus; basal lamina; nucleus

Molecular Function: heparin binding; rRNA binding; protein binding; copper ion binding; DNA binding; endonuclease activity; actin binding; peptide binding; receptor binding; ribonuclease activity

Biological Process: cell migration; diacylglycerol biosynthetic process; negative regulation of smooth muscle cell proliferation; activation of protein kinase B; activation of phospholipase A2; response to hormone stimulus; oocyte maturation; rRNA transcription; ovarian follicle development; homeostatic process; positive regulation of protein secretion; actin filament polymerization; phospholipase C activation; negative regulation of translation; response to hypoxia; cell communication; positive regulation of endothelial cell proliferation; angiogenesis; positive regulation of phosphorylation; placenta development

Disease: Amyotrophic Lateral Sclerosis 9
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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