• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

ANG1 clia kit :: Human ANG1 (Angiopoietin 1) CLIA Kit

Scan QR to view Datasheet
Catalog # MBS2531014
Unit / Price
Scan QR to view Datasheet
  48-Strip-Wells  /  $450 +1 FREE 8GB USB
  96-Strip-Wells  /  $535 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,255 +2 FREE 8GB USB
  10x96-Strip-Wells  /  $3,975 +4 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

ANG1 (Angiopoietin 1), CLIA Kit

Full Product Name

Human ANG1 (Angiopoietin 1) CLIA Kit

Product Synonym Names
ANG-1; ANGPT1; AGP1; AGPT
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
OMIM
601667
Species Reactivity
Specificity
This kit recognizes natural and recombinant ANG1. No significant cross-reactivity or interference between ANG1 and analogues was observed.
Assay Type
Sandwich
Detection Range
15.625-1000pg/mL
Sensitivity
Min: 9.375pg/mL; Max: 1000pg/mL
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Product Note
Our CLIA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of ANG1 clia kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
ANG1 clia kit
Intended Uses: This CLIA kit applies to the in vitro quantitative determination of ANG1 concentrations in serum, plasma and other biological fluids.

Principle of the Assay: This kit uses Sandwich-CLIA as the method. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to ANG1. Standards or samples are added to the appropriate micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for ANG1 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain ANG1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured spectrophotometrically by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of ANG1. You can calculate the concentration of ANG1 in the samples by comparing the RLU value of the samples to the standard curve.

Typical Testing Data/Standard Curve (for reference only) of ANG1 clia kit
ANG1 clia kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS2531014. Click to request current manual
NCBI/Uniprot data below describe general gene information for ANG1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
57,456 Da
NCBI Official Full Name
angiopoietin-1 isoform 1
NCBI Official Synonym Full Names
angiopoietin 1
NCBI Official Symbol
ANGPT1  [Similar Products]
NCBI Official Synonym Symbols
AGP1; AGPT; ANG1
  [Similar Products]
NCBI Protein Information
angiopoietin-1
UniProt Protein Name
Angiopoietin-1
Protein Family
UniProt Gene Name
ANGPT1  [Similar Products]
UniProt Synonym Gene Names
KIAA0003; ANG-1  [Similar Products]
UniProt Entry Name
ANGP1_HUMAN
NCBI Summary for ANG1
This gene encodes a secreted glycoprotein that belongs to the angiopoietin family. Members of this family play important roles in vascular development and angiogenesis. All angiopoietins bind with similar affinity to an endothelial cell-specific tyrosine-protein kinase receptor. The protein encoded by this gene is a secreted glycoprotein that activates the receptor by inducing its tyrosine phosphorylation. It plays a critical role in mediating reciprocal interactions between the endothelium and surrounding matrix and mesenchyme and inhibits endothelial permeability. The protein also contributes to blood vessel maturation and stability, and may be involved in early development of the heart. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Sep 2015]
UniProt Comments for ANG1
ANGPT1: Binds and activates TEK/TIE2 receptor by inducing its dimerization and tyrosine phosphorylation. Plays an important role in the regulation of angiogenesis, endothelial cell survival, proliferation, migration, adhesion and cell spreading, reorganization of the actin cytoskeleton, but also maintenance of vascular quiescence. Required for normal angiogenesis and heart development during embryogenesis. After birth, activates or inhibits angiogenesis, depending on the context. Inhibits angiogenesis and promotes vascular stability in quiescent vessels, where endothelial cells have tight contacts. In quiescent vessels, ANGPT1 oligomers recruit TEK to cell-cell contacts, forming complexes with TEK molecules from adjoining cells, and this leads to preferential activation of phosphatidylinositol 3-kinase and the AKT1 signaling cascades. In migrating endothelial cells that lack cell-cell adhesions, ANGT1 recruits TEK to contacts with the extracellular matrix, leading to the formation of focal adhesion complexes, activation of PTK2/FAK and of the downstream kinases MAPK1/ERK2 and MAPK3/ERK1, and ultimately to the stimulation of sprouting angiogenesis. Mediates blood vessel maturation/stability. Implicated in endothelial developmental processes later and distinct from that of VEGF. Appears to play a crucial role in mediating reciprocal interactions between the endothelium and surrounding matrix and mesenchyme.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 8q23.1

Cellular Component: extracellular space; microvillus; plasma membrane; extracellular region; intracellular; lipid raft

Molecular Function: protein binding; receptor tyrosine kinase binding

Biological Process: axon guidance; transmembrane receptor protein tyrosine kinase activation (dimerization); positive regulation of cell adhesion; nerve growth factor receptor signaling pathway; activation of MAPKK activity; positive regulation of receptor internalization; negative regulation of protein import into nucleus; negative regulation of cell adhesion; negative regulation of protein amino acid phosphorylation; positive chemotaxis; small GTPase mediated signal transduction; hemopoiesis; negative regulation of neuron apoptosis; regulation of I-kappaB kinase/NF-kappaB cascade; epidermal growth factor receptor signaling pathway; Tie receptor signaling pathway; regulation of endothelial cell proliferation; fibroblast growth factor receptor signaling pathway; in utero embryonic development; positive regulation of blood vessel endothelial cell migration; MAPKKK cascade; positive regulation of peptidyl-serine phosphorylation; cell-substrate adhesion; negative regulation of cytokine secretion during immune response; positive regulation of phosphoinositide 3-kinase cascade; positive regulation of protein kinase B signaling cascade; positive regulation of peptidyl-tyrosine phosphorylation; heparin biosynthetic process; positive regulation of protein ubiquitination; regulation of protein binding; Ras protein signal transduction; insulin receptor signaling pathway; negative regulation of vascular permeability; innate immune response; regulation of tumor necrosis factor production; regulation of satellite cell proliferation; sprouting angiogenesis; blood coagulation; vascular endothelial growth factor receptor signaling pathway; leukocyte migration; negative regulation of apoptosis
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000