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ANGPTL3 elisa kit :: Mouse ANGPTL3 ELISA Kit

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Catalog # MBS177160
Unit / Price
  96-Strip-Wells  /  $425 +1 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

ANGPTL3, ELISA Kit

Popular Item
Also Known As

Mouse ANGPTL3 PicoKine ELISA Kit

Product Synonym Names
Angiopoietin-related protein 3; angiopoietin-like 3; ANG 5; ANG-5; ANG5; Angiopoietin 5; Angiopoietin like 3; Angiopoietin related protein 3; Angiopoietin-5; Angiopoietin-like protein 3; Angiopoietin-related protein 3; ANGL3_HUMAN; ANGPT5; ANGPTL3; ANL3; FHBL2; OTTHUMP00000010719
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
Sequence Length
455
3D Structure
ModBase 3D Structure for Q9R182
Species Reactivity
Specificity
Natural and recombinant mouse ANGPTL3
Samples
Cell culture supernates, serum and plasma (heparin, EDTA)
Assay Type
Sandwich
Detection Range
156pg/ml-10,000pg/ml
Sensitivity
< 10pg/ml
Intra-assay Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-assay Precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Preparation and Storage
Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of ANGPTL3 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forANGPTL3purchase
MBS177160 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the ANGPTL3, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing ANGPTL3. The ELISA analytical biochemical technique of the MBS177160 kit is based on ANGPTL3 antibody-ANGPTL3 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ANGPTL3 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ANGPTL3. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
ANGPTL3 elisa kit
Principle of the assay: mouse ANGPTL3 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for ANGPTL3 has been precoated onto 96-well plates. Standards(sf21, S17-T455) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for ANGPTL3 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse ANGPTL3 amount of sample captured in plate.
Background: ANGPTL3, also known as Angiopoietin-like 3 or HYPLl, is a protein that in humans is encoded by the ANGPTL3 gene. It is mapped to 1p31.3. The protein encoded by this gene is a member of the angiopoietin-like family of secreted factors. It is expressed predominantly in the liver, and has the characteristic structure of angiopoietins, consisting of a signal peptide, N-terminal coiled-coil domain, and the C-terminal fibrinogen (FBN)-like domain. ANGPTL3 also acts as dual inhibitor of lipoprotein lipase (LPL) and endothelial lipase (EL), and increases plasma triglyceride and HDL cholesterol in rodents. It can inhibit endothelial lipase to catalyze HDL-phospholipid and increase HDL-PL levels.

Typical Testing Data/Standard Curve (for reference only) of ANGPTL3 elisa kit
Mouse ANGPTL3 PicoKine ELISA Kit standard curve
ANGPTL3 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS177160. Click to request current manual
NCBI/Uniprot data below describe general gene information for ANGPTL3. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Related Accession #
Molecular Weight
52,543 Da
NCBI Official Full Name
Angiopoietin-related protein 3
NCBI Official Synonym Full Names
angiopoietin-like 3
NCBI Official Symbol
Angptl3  [Similar Products]
NCBI Official Synonym Symbols
NCBI Protein Information
angiopoietin-related protein 3; hypolipidemia; angiopoietin-like protein 3
UniProt Protein Name
Angiopoietin-related protein 3
UniProt Synonym Protein Names
Angiopoietin-like protein 3
UniProt Gene Name
Angptl3  [Similar Products]
UniProt Entry Name
ANGL3_MOUSE
NCBI Summary for ANGPTL3
This gene encodes a member of the angiopoietin-like family of proteins. The encoded preproprotein is proteolytically processed to generate multiple protein products, which may inhibit triglyceride metabolism. Homozygous knockout mice for this gene exhibit reduced plasma lipid concentrations, including reduced plasma triglyceride concentrations, and enhanced activity of enzymes involved in triglyceride metabolism. [provided by RefSeq, Aug 2015]
UniProt Comments for ANGPTL3
ANGPTL3: Defects in ANGPTL3 are the cause of familial hypobetalipoproteinemia type 2 (FHBL2); also called combined hypobetalipoproteinemia familial. FHBL2 is a disorder of lipid metabolism characterized by less than 5th percentile age- and sex-specific levels of low density lipoproteins, and dietary fat malabsorption. Affected individuals present with combined hypolipidemia, consisting of extremely low plasma levels of LDL cholesterol, HDL cholesterol, and triglycerides.

Protein type: Cell adhesion; Secreted, signal peptide; Secreted; Motility/polarity/chemotaxis; Inhibitor

Cellular Component: Golgi apparatus; extracellular space; cell surface; early endosome; extracellular region

Molecular Function: integrin binding; enzyme inhibitor activity; phospholipase inhibitor activity; growth factor activity

Biological Process: cholesterol metabolic process; phospholipid catabolic process; glycerol metabolic process; cell-matrix adhesion; lipid homeostasis; positive regulation of lipid catabolic process; fatty acid metabolic process; signal transduction; positive regulation of angiogenesis; sequestering of lipid; cholesterol homeostasis; triacylglycerol metabolic process; acylglycerol homeostasis; phospholipid metabolic process; negative regulation of catalytic activity; artery morphogenesis; positive regulation of lipid metabolic process; negative regulation of lipoprotein lipase activity; positive regulation of cell migration; phospholipid homeostasis
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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