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ANT elisa kit :: Mouse adenine nucleotide translocator (ANT) autoantibody ELISA Kit

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Catalog # MBS706514
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Typical Testing Data/Standard Curve (for reference only)
Product Name

adenine nucleotide translocator (ANT) autoantibody, ELISA Kit

Popular Item
Full Product Name

Mouse adenine nucleotide transloCator, ANT autoantibody ELISA Kit

Product Synonym Names
Mouse adenine nucleotide translocator (ANT) autoantibody ELISA kit; adenine nucleotide translocator (ANT) autoantibody
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
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MBS706514 Testing Data
3D Structure
ModBase 3D Structure for P12857
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of mouse ANT autoantibody. No significant cross-reactivity or interference between mouse ANT autoantibody and analogues was observed.
Samples
Serum, plasma
Assay Type
Sandwich
Detection Range
0.156 ng/ml -10 ng/ml.
Sensitivity
The minimum detectable dose of mouse ANT autoantibody is typically less than 0.039 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of ANT elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forANTpurchase
MBS706514 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the adenine nucleotide translocator (ANT) autoantibody, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing ANT. The ELISA analytical biochemical technique of the MBS706514 kit is based on ANT antibody-ANT antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ANT antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ANT. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
ANT elisa kit
Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with ANT. Standards and samples are pipetted into the wells with a biotin-conjugated ANT and avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ANT autoantibody bound in the initial step. The color development is stopped and the intensity of the color is measured.

Typical Testing Data/Standard Curve (for reference only) of ANT elisa kit
ANT elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS706514. Click to request current manual
NCBI/Uniprot data below describe general gene information for ANT. It may not necessarily be applicable to this product.
NCBI GI #
NCBI Accession #
UniProt Primary Accession #
Molecular Weight
42,333 Da
NCBI Official Full Name
adenine nucleotide translocator
UniProt Protein Name
ADP,ATP carrier protein 2, mitochondrial
UniProt Synonym Protein Names
ADP/ATP translocase 2; Adenine nucleotide translocator 2; ANT 2
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
ANT-G2; ANT 2  [Similar Products]
UniProt Entry Name
ADT2_MAIZE
UniProt Comments for ANT
Function: Catalyzes the exchange of ADP and ATP across the mitochondrial inner membrane.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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