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ANXA1 recombinant protein :: Annexin A1 (ANXA1) Recombinant Protein

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Catalog # MBS2011941 (SPECIAL PROMOTION: Get FREE $5 Starbucks gift card (Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
Unit / Price
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  0.01 mg  /  $115 +1 FREE 8GB USB
  0.05 mg  /  $200 +1 FREE 8GB USB
  0.1 mg  /  $290 +1 FREE 8GB USB
  0.2 mg  /  $360 +1 FREE 8GB USB
  0.5 mg  /  $690 +1 FREE 8GB USB
  1 mg  /  $1,015 +1 FREE 8GB USB
SDS-Page
Product Name

Annexin A1 (ANXA1), Recombinant Protein

Full Product Name

Recombinant Annexin A1 (ANXA1)

Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Sequence
The target protein is fused with two N-terminal Tags, His-tag and S-tag, its sequence is listed below.
MHHHHHHSSG LVPRGSGMKE TAAAKFERQH MDSPDLGTDD DDKAMADIGS -MAMVSEFLK Q AWFIDNEEQD YINTVKTYKG GPGSAVSPYP AFNPSSDVAA LHQAIMVKGV DEATIIDILT KRNNAQRQQI KAAYLQEKGK PLDEVLKKAL TGHLEEVVLA LLKTPAQFDA DELRAAMKGL GTDEDTLIEI LASRNNKEIR EINRVYREEL KRDLAKDIAS DTSGDFQKAL LSLAKGDRSE DFGVNEDLAD TDARALYEAG ERRKGADVNV FTTILTTRSY LHLRRVFQKY SKYSQHDMNK VLDLELKGDI EKCLTAIVQC ATCKPAYFAE KLYQAMKGAG TRHKALIRIM VSRSEVDMND IKAFYQKKYG VSLCQAILDE TKGDYEKILV ALCGGN
Chromosome Location
Chromosome: 1; NC_013669.1 (59691471..59709374). Location: chromosome: 1
3D Structure
ModBase 3D Structure for P51662
Host
E Coli
Purity/Purification
> 95%
Form/Format
Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
Organism
Oryctolagus cuniculus (Rabbit)
Expression System
Prokaryotic expression
Residues
Met1~Asn346 (Accession # P51662) with two N-terminal Tags, His-tag and S-tag
Endotoxin Level
<1.0EU per 1ug (determined by the LAL method)
Reconstitution
Reconstitute in sterile PBS, pH7.2-pH7.4.
Preparation and Storage
Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.
Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.
ISO Certification
Manufactured in an ISO 9001:2008 and ISO 13485:2003 Certified Laboratory.
Supply Chain Verification
Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customer’s specifications, please inquire.
Other Notes
Small volumes of ANXA1 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
ANXA1 recombinant protein
About the Marker: Effective Size Range: 10kDa to 70kDa.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at doubleintensity to make location and size approximation of proteins of interestquick and easy.
Ready-to-use: No need to heat, dilute or add reducing agents before use.
Applications Tested/Suitable for ANXA1 recombinant protein
SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)

NCBI/Uniprot data below describe general gene information for ANXA1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
44.2kDa
NCBI Official Full Name
annexin A1
NCBI Official Symbol
ANXA1  [Similar Products]
NCBI Protein Information
annexin A1; p35; annexin I; annexin-1; calpactin-2; calpactin II; lipocortin I; chromobindin-9; phospholipase A2 inhibitory protein
UniProt Protein Name
Annexin A1
UniProt Synonym Protein Names
Annexin I; Annexin-1; Calpactin II; Calpactin-2; Chromobindin-9; Lipocortin I; Phospholipase A2 inhibitory protein; p35
Protein Family
UniProt Gene Name
ANXA1  [Similar Products]
UniProt Synonym Gene Names
UniProt Entry Name
ANXA1_RABIT
UniProt Comments for ANXA1
Function: Calcium/phospholipid-binding protein which promotes membrane fusion and is involved in exocytosis. This protein regulates phospholipase A2 activity. It seems to bind from two to four calcium ions with high affinity.

Subunit structure: Homodimer in placenta (20%); linked by transglutamylation. Interacts with DYSF

By similarity.

Subcellular location: Nucleus. Cytoplasm. Cell projection › cilium. Basolateral cell membrane. Note: Found in the cilium, nucleus and basolateral cell membrane of ciliated cells in the tracheal endothelium. Found in the cytoplasm of type II pneumocytes and alveolar macrophages. Ref.2

Tissue specificity: In the lung, expressed in the ciliated cells of the tracheal endothelium, but not in the goblet cells. Expressed in type II pneumocytes and alveolar macrophages. Ref.2

Domain: A pair of annexin repeats may form one binding site for calcium and phospholipid.

Post-translational modification: Phosphorylated by protein kinase C, epidermal growth factor receptor/kinase and TRPM7. Phosphorylation results in loss of the inhibitory activity

By similarity.

Sequence similarities: Belongs to the annexin family.Contains 4 annexin repeats.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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