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ANXA2 elisa kit :: Human Annexin A2 ELISA Kit

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Catalog # MBS732593
Unit / Price
  48-Strip-Wells  /  $440 +1 FREE 8GB USB
  96-Strip-Wells  /  $640 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,895 +3 FREE 8GB USB
  10x96-Strip-Wells  /  $5,415 +6 FREE 8GB USB
Typical Standard Curve/Testing Data
Product Name

Annexin A2 (ANXA2), ELISA Kit

Popular Item
Full Product Name

Human Annexin A2 ELISA Kit

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
OMIM
151740
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of ANXA2. No significant cross-reactivity or interference between ANXA2 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between ANXA2 and all the analogues, therefore, cross reaction may still exist in some cases.
Samples
Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type
Competitive
Detection Range
10-250ng/mL
Sensitivity
1.0ng/mL
Preparation and Storage
Store all reagents at 2-8 degree C.
Sample Preparation
We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of ANXA2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forANXA2purchase
MBS732593 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Annexin A2 (ANXA2) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing ANXA2. The ELISA analytical biochemical technique of the MBS732593 kit is based on ANXA2 antibody-ANXA2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ANXA2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ANXA2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
ANXA2 elisa kit
Intended Uses: This ANXA2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human ANXA2. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Principle of the Assay: ANXA2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ANXA2 antibody and an ANXA2-HRP conjugate. The assay sample and buffer are incubated together with ANXA2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ANXA2 concentration since ANXA2 from samples and ANXA2-HRP conjugate compete for the anti-ANXA2 antibody binding site. Since the number of sites is limited, as more sites are occupied by ANXA2 from the sample, fewer sites are left to bind ANXA2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ANXA2 concentration in each sample is interpolated from this standard curve.
Product Categories/Family for ANXA2 elisa kit

Typical Standard Curve/Testing Data of ANXA2 elisa kit
ANXA2 elisa kit Typical Standard Curve/Testing Data image
Sample Manual Insert of MBS732593. Click to request current manual
NCBI/Uniprot data below describe general gene information for ANXA2. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
40,411 Da
NCBI Official Full Name
annexin A2 isoform 2
NCBI Official Synonym Full Names
annexin A2
NCBI Official Symbol
ANXA2  [Similar Products]
NCBI Official Synonym Symbols
P36; ANX2; LIP2; LPC2; CAL1H; LPC2D; ANX2L4; PAP-IV; HEL-S-270
  [Similar Products]
NCBI Protein Information
annexin A2; annexin-2; protein I; annexin II; lipocortin II; chromobindin 8; chromobindin-8; calpactin I heavy chain; calpactin-1 heavy chain; calpactin I heavy polypeptide; placental anticoagulant protein IV; epididymis secretory protein Li 270
UniProt Protein Name
Annexin A2
UniProt Synonym Protein Names
Annexin II; Annexin-2; Calpactin I heavy chain; Calpactin-1 heavy chain; Chromobindin-8; Lipocortin II; Placental anticoagulant protein IV; PAP-IV; Protein I; p36
Protein Family
UniProt Gene Name
ANXA2  [Similar Products]
UniProt Synonym Gene Names
ANX2; ANX2L4; CAL1H; LPC2D; PAP-IV  [Similar Products]
UniProt Entry Name
ANXA2_HUMAN
NCBI Summary for ANXA2
This gene encodes a member of the annexin family. Members of this calcium-dependent phospholipid-binding protein family play a role in the regulation of cellular growth and in signal transduction pathways. This protein functions as an autocrine factor which heightens osteoclast formation and bone resorption. This gene has three pseudogenes located on chromosomes 4, 9 and 10, respectively. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
UniProt Comments for ANXA2
ANXA2: a calcium-regulated membrane-binding protein whose affinity for calcium is greatly enhanced by anionic phospholipids. It binds two calcium ions with high affinity. Heterotetramer containing 2 light chains of S100A10 2 heavy chains of ANXA2. May cross-link plasma membrane phospholipids with actin and the cytoskeleton and be involved with exocytosis. Annexins are a family of structurally related proteins whose common property is calcium-dependent binding to phospholipids. There are at least ten different annexins in mammalian species. Annexins do not contain signal peptides, yet some annexins (A1, A2 and A5) appear to be secreted in a physiologically regulated fashion.

Protein type: Calcium-binding; Motility/polarity/chemotaxis; Lipid-binding

Chromosomal Location of Human Ortholog: 15q22.2

Cellular Component: extrinsic to plasma membrane; extracellular space; protein complex; cell surface; lysosomal membrane; late endosome membrane; early endosome; lipid particle; cell cortex; lipid raft; extracellular matrix; ruffle; membrane; perinuclear region of cytoplasm; melanosome; plasma membrane; basement membrane; midbody; nucleus; endosome; sarcolemma; vesicle

Molecular Function: protein binding; phosphatidylinositol-4,5-bisphosphate binding; calcium-dependent phospholipid binding; phospholipase A2 inhibitor activity; cytoskeletal protein binding; calcium ion binding; calcium-dependent protein binding; Rab GTPase binding

Biological Process: positive regulation of fibroblast proliferation; fibrinolysis; collagen fibril organization; negative regulation of catalytic activity; protein heterotetramerization; positive regulation of vesicle fusion; angiogenesis; positive regulation of binding; positive regulation of protein amino acid phosphorylation; body fluid secretion; lipid raft formation; membrane budding
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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