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ANXA5 kit :: ANNEXIN V KIT

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Catalog # MBS239211
Unit / Price
  100 Tests  /  $490 +1 FREE 8GB USB
Testing Data #1
Product Name

ANNEXIN V KIT (ANXA5), Kit

Popular Item
Also Known As

ANNEXIN V:Biotin ASSAY KIT

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
Sequence Length
320
OMIM
131230
3D Structure
ModBase 3D Structure for P08758
Form/Format
Biotin
Reagents In The Kits
Annexin V:Biotin 1 x 0.5ml vial, Propidium Iodide 1 x 1.6ml vial at 20ug/ml, Binding buffer 1 x 50ml vial at x 4 concentrate
Note: This assay also requires streptavidin:FITC conjugate for visualisation (not supplied - see recommended useful reagents section).
Perservative Stabilisers
Preparation and Storage
Store at 4 degree C. DO NOT FREEZE. This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 12 months from date of despatch.
Product Note
Our Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of ANXA5 kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
ANXA5 kit
This test employs the property of Annexin V:Biotin to bind to the membrane phospholipid phosphatidylserine (PS) in the presence of Ca2+. PS is exposed at the cell surface during the early stages of apoptosis. Detection of PS is a very sensitive method for detecting cells entering apoptosis, at a time point considerably ahead of nuclear changes such as DNA degradation. The conjugation protocol used to prepare Annexin V:Biotin has not changed the native phospholipid binding properties of Annexin V. This protocol is designed to measure apoptosis easily and quickly in a sample of suspended cells.
Applications Tested/Suitable for ANXA5 kit
Flow cytometry (FC/FACS)
Application Notes for ANXA5 kit
Flow Cytometry: Maximum Dilution: Neat

Testing Data #1 of ANXA5 kit
Dot-plot showing Ramos cells staining with Annexin V:FITC (MBS239205) versus Propidium Iodide resulting in three distinct populations
ANXA5 kit Testing Data #1 image
NCBI/Uniprot data below describe general gene information for ANXA5. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
35,937 Da[Similar Products]
NCBI Official Full Name
annexin A5
NCBI Official Synonym Full Names
annexin A5
NCBI Official Symbol
ANXA5  [Similar Products]
NCBI Official Synonym Symbols
PP4; ANX5; ENX2; RPRGL3; HEL-S-7
  [Similar Products]
NCBI Protein Information
annexin A5; CBP-I; PAP-I; VAC-alpha; anchorin CII; annexin V; annexin-5; calphobindin I; endonexin II; epididymis secretory protein Li 7; lipocortin V; placental anticoagulant protein 4; placental anticoagulant protein I; thromboplastin inhibitor; vascular anticoagulant-alpha
UniProt Protein Name
Annexin A5
UniProt Synonym Protein Names
Anchorin CII; Annexin V; Annexin-5; Calphobindin I; CBP-I; Endonexin II; Lipocortin V; Placental anticoagulant protein 4; PP4; Placental anticoagulant protein I; PAP-I; Thromboplastin inhibitor; Vascular anticoagulant-alpha; VAC-alpha
Protein Family
UniProt Gene Name
ANXA5  [Similar Products]
UniProt Synonym Gene Names
ANX5; ENX2; PP4; CBP-I; PP4; PAP-I; VAC-alpha  [Similar Products]
UniProt Entry Name
ANXA5_HUMAN
NCBI Summary for ANXA5
The protein encoded by this gene belongs to the annexin family of calcium-dependent phospholipid binding proteins some of which have been implicated in membrane-related events along exocytotic and endocytotic pathways. Annexin 5 is a phospholipase A2 and protein kinase C inhibitory protein with calcium channel activity and a potential role in cellular signal transduction, inflammation, growth and differentiation. Annexin 5 has also been described as placental anticoagulant protein I, vascular anticoagulant-alpha, endonexin II, lipocortin V, placental protein 4 and anchorin CII. The gene spans 29 kb containing 13 exons, and encodes a single transcript of approximately 1.6 kb and a protein product with a molecular weight of about 35 kDa. [provided by RefSeq, Jul 2008]
UniProt Comments for ANXA5
ANXA5: a calcium/phospholipid-binding protein and an anticoagulant protein that acts as an indirect inhibitor of the thromboplastin-specific complex, which is involved in the blood coagulation cascade. The binding of labeled ANXA5 to phosphatidylserine is used as a marker of apoptosis. Annexins are a family of structurally related proteins whose common property is calcium-dependent binding to phospholipids. There are at least ten different annexins in mammalian species. Annexins do not contain signal peptides, yet some annexins (A1, A2 and A5) appear to be secreted in a physiologically regulated fashion.

Protein type: Inhibitor; Apoptosis; Lipid-binding; Calcium-binding

Chromosomal Location of Human Ortholog: 4q27

Cellular Component: focal adhesion; membrane; cytoplasm; intracellular; external side of plasma membrane

Molecular Function: phospholipase inhibitor activity; calcium-dependent phospholipid binding; phospholipid binding; calcium ion binding

Biological Process: response to organic substance; negative regulation of catalytic activity; signal transduction; blood coagulation; negative regulation of coagulation; negative regulation of apoptosis

Disease: Pregnancy Loss, Recurrent, Susceptibility To, 3
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Pathways associated with ANXA5 kit
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