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AP-1 elisa kit :: Porcine Transcription Factor / Activator Protein 1 (AP-1) ELISA Kit

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Catalog # MBS269963
Unit / Price
  48-Strip-Wells  /  $300 +1 FREE 8GB USB
  96-Strip-Wells  /  $475 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $1,825 +2 FREE 8GB USB
  10x96-Strip-Wells  /  $3,425 +4 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

Transcription Factor / Activator Protein 1 (AP-1), ELISA Kit

Popular Item
Also Known As

Porcine Transcription Factor / Activator Protein 1 (AP-1) ELISA Kit

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
Sequence Length
347
OMIM
165161
Species Reactivity
Specificity
No cross-reaction with other factors.
Samples
Porcine serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant AP-1 concentration
Assay Type
Sandwich
Detection Range
10 ng/ml-0.156ng/ml
Sensitivity
0.05 ng/ml.
Intra-assay Precision
<= 8%
Inter-assay Precision
<= 12%
Preparation and Storage
Store all reagents at 2-8 degree C.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of AP-1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forAP-1purchase
MBS269963 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Transcription Factor / Activator Protein 1 (AP-1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing AP-1. The ELISA analytical biochemical technique of the MBS269963 kit is based on AP-1 antibody-AP-1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect AP-1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, AP-1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
AP-1 elisa kit
Principle of the Assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Porcine AP-1 monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

Typical Testing Data/Standard Curve (for reference only) of AP-1 elisa kit
AP-1 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS269963. Click to request current manual
NCBI/Uniprot data below describe general gene information for AP-1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
35,879 Da
NCBI Official Full Name
transcription factor jun-B
NCBI Official Synonym Full Names
jun B proto-oncogene
NCBI Official Symbol
NCBI Official Synonym Symbols
NCBI Protein Information
transcription factor jun-B; activator protein 1
UniProt Protein Name
Transcription factor jun-B
UniProt Gene Name
UniProt Entry Name
JUNB_HUMAN
UniProt Comments for AP-1
JunB: Transcription factor involved in regulating gene activity following the primary growth factor response. Binds to the DNA sequence 5'-TGA[CG]TCA-3'. Binds DNA as an homodimer or as an heterodimer with another member of the Jun/Fos family. Interacts with ITCH (via its WW domains). By growth factors. Belongs to the bZIP family. Jun subfamily.

Protein type: Oncoprotein; Motility/polarity/chemotaxis; Transcription factor

Chromosomal Location of Human Ortholog: 19p13.2

Cellular Component: nucleoplasm; chromatin

Molecular Function: protein binding; DNA binding; double-stranded DNA binding; transcription coactivator activity; transcription corepressor activity; transcription factor activity

Biological Process: response to drug; response to peptide hormone stimulus; response to light stimulus; transcription initiation from RNA polymerase II promoter; response to cAMP; transcription, DNA-dependent; regulation of cell cycle; embryonic process involved in female pregnancy; decidualization; osteoclast differentiation; trophectodermal cell differentiation; cellular response to hormone stimulus; osteoblast differentiation; regulation of transcription from RNA polymerase II promoter; osteoblast proliferation; response to mechanical stimulus; response to cytokine stimulus; transforming growth factor beta receptor signaling pathway; gene expression; positive regulation of transcription from RNA polymerase II promoter; response to progesterone stimulus; positive regulation of cell differentiation; vasculogenesis; response to corticosterone stimulus
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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