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AP-12 elisa kit :: Rat Apelin-12 (AP-12) ELISA Kit

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Catalog # MBS269440
Unit / Price
  48-Strip-Wells  /  $275 +1 FREE 8GB USB
  96-Strip-Wells  /  $435 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $1,755 +2 FREE 8GB USB
  10x96-Strip-Wells  /  $3,150 +3 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

Apelin-12 (AP-12), ELISA Kit

Popular Item
Full Product Name

Rat Apelin-12 (AP-12) ELISA Kit

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
OMIM
600052
Species Reactivity
Specificity
No cross-reaction with other factors.
Samples
Rat serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant AP-12 concentration
Assay Type
Sandwich
Detection Range
2000 pg/ml-31.2 pg/ml
Sensitivity
12 pg/ml.
Intra-assay Precision
? 8%
Inter-assay Precision
? 12%
Preparation and Storage
Store all reagents at 2-8 degree C.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of AP-12 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forAP-12purchase
MBS269440 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Apelin-12 (AP-12) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing AP-12. The ELISA analytical biochemical technique of the MBS269440 kit is based on AP-12 antibody-AP-12 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect AP-12 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, AP-12. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
AP-12 elisa kit
Principle of the assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Rat AP-12 monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

Typical Testing Data/Standard Curve (for reference only) of AP-12 elisa kit
AP-12 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS269440. Click to request current manual
NCBI/Uniprot data below describe general gene information for AP-12. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Related Accession #
Molecular Weight
42,660 Da
NCBI Official Full Name
apelin receptor
NCBI Official Synonym Full Names
apelin receptor
NCBI Official Symbol
APLNR  [Similar Products]
NCBI Official Synonym Symbols
APJ; APJR; HG11; AGTRL1
  [Similar Products]
NCBI Protein Information
apelin receptor; APJ receptor; HG11 orphan receptor; APJ (apelin) receptor; angiotensin receptor-like 1; G protein-coupled receptor APJ; G-protein coupled receptor APJ; angiotensin II receptor-like 1; G-protein coupled receptor HG11
UniProt Protein Name
Apelin receptor
UniProt Synonym Protein Names
Angiotensin receptor-like 1; G-protein coupled receptor APJ; G-protein coupled receptor HG11
UniProt Gene Name
APLNR  [Similar Products]
UniProt Synonym Gene Names
AGTRL1; APJ  [Similar Products]
UniProt Entry Name
APJ_HUMAN
NCBI Summary for AP-12
This gene encodes a member of the G protein-coupled receptor gene family. The encoded protein is related to the angiotensin receptor, but is actually an apelin receptor that inhibits adenylate cyclase activity and plays a counter-regulatory role against the pressure action of angiotensin II by exerting hypertensive effect. It functions in the cardiovascular and central nervous systems, in glucose metabolism, in embryonic and tumor angiogenesis and as a human immunodeficiency virus (HIV-1) coreceptor. Two transcript variants resulting from alternative splicing have been identified. [provided by RefSeq, Jul 2009]
UniProt Comments for AP-12
AGTRL1: Receptor for apelin coupled to G proteins that inhibit adenylate cyclase activity. Alternative coreceptor with CD4 for HIV-1 infection; may be involved in the development of AIDS dementia. Belongs to the G-protein coupled receptor 1 family.

Protein type: Membrane protein, integral; Membrane protein, multi-pass; Receptor, GPCR; GPCR, family 1

Chromosomal Location of Human Ortholog: 11q12

Cellular Component: integral to plasma membrane; plasma membrane

Molecular Function: G-protein coupled receptor activity; receptor activity

Biological Process: G-protein coupled receptor protein signaling pathway; regulation of body fluid levels; heart development; gastrulation
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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