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APAF1 elisa kit :: Rat apoptotic peptidase activating factor 1 ELISA Kit

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Catalog # MBS9320828
Unit / Price
  48-Strip-Wells  /  $470 +1 FREE 8GB USB
  96-Strip-Wells  /  $680 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $3,100 +3 FREE 8GB USB
  10x96-Strip-Wells  /  $6,095 +7 FREE 8GB USB
APAF1 elisa kit
Product Name

apoptotic peptidase activating factor 1 (APAF1), ELISA Kit

Also Known As

Rat Apoptotic protease-activating factor 1, APAF1 ELISA Kit

Product Synonym Names
Rat Apoptotic protease-activating factor 1 (APAF1) ELISA kit; APAF-1; CED4; DKFZp781B1145; apoptotic protease activating factor 1; apoptotic peptidase activating factor 1
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
3D Structure
ModBase 3D Structure for Q9EPV5
Species Reactivity
Preparation and Storage
Store all reagents at 2-8 degree C
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of APAF1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forAPAF1purchase
MBS9320828 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the apoptotic peptidase activating factor 1 (APAF1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing APAF1. The ELISA analytical biochemical technique of the MBS9320828 kit is based on APAF1 antibody-APAF1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect APAF1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, APAF1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
NCBI/Uniprot data below describe general gene information for APAF1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Related Accession #
Molecular Weight
141,152 Da
NCBI Official Full Name
apoptotic protease-activating factor 1
NCBI Official Synonym Full Names
apoptotic peptidase activating factor 1
NCBI Official Symbol
Apaf1  [Similar Products]
NCBI Protein Information
apoptotic protease-activating factor 1; apaf-1; apoptotic protease activating factor 1
UniProt Protein Name
Apoptotic protease-activating factor 1
UniProt Gene Name
Apaf1  [Similar Products]
UniProt Synonym Gene Names
APAF-1  [Similar Products]
UniProt Entry Name
APAF_RAT
NCBI Summary for APAF1
protein involved in the initiation of apoptosis [RGD, Feb 2006]
UniProt Comments for APAF1
APAF: Oligomeric Apaf-1 mediates the cytochrome c-dependent autocatalytic activation of pro-caspase-9 (Apaf-3), leading to the activation of caspase-3 and apoptosis. This activation requires ATP. Isoform 6 is less effective in inducing apoptosis. Monomer. Oligomerizes upon binding of cytochrome c and dATP. Oligomeric Apaf-1 and pro-caspase-9 bind to each other via their respective NH2-terminal CARD domains and consecutively mature caspase-9 is released from the complex. Pro-caspase-3 is recruited into the Apaf-1-pro-caspase-9 complex via interaction with pro-caspase-9. Interacts with APIP. Interacts (via CARD and NACHT domains) with NAIP/BIRC1 (via NACHT domain). By E2F and p53/TP53 in apoptotic neurons. Ubiquitous. Highest levels of expression in adult spleen and peripheral blood leukocytes, and in fetal brain, kidney and lung. Isoform 1 is expressed in heart, kidney and liver. 6 isoforms of the human protein are produced by alternative splicing.

Protein type: Apoptosis

Cellular Component: cytosol; nucleus; apoptosome

Molecular Function: identical protein binding; protein binding; heat shock protein binding; caspase activator activity; ADP binding; ATP binding

Biological Process: caspase activation; apoptosis; multicellular organismal development; neuron apoptosis; neural tube closure; forebrain development; response to hypoxia; caspase activation via cytochrome c; brain development; cell differentiation; response to nutrient; protein homooligomerization; aging
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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