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APAF1 rtu elisa kit :: Rat Apoptotic Peptidase Activating Factor 1 (APAF1) RTU ELISA Kit

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Catalog # MBS457672
Unit / Price
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  48-Strip-Wells  /  $425 +1 FREE 8GB USB
  96-Strip-Wells  /  $570 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,600 +3 FREE 8GB USB
  10x96-Strip-Wells  /  $5,050 +6 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

Apoptotic Peptidase Activating Factor 1 (APAF1), RTU ELISA Kit

Full Product Name

Rat Apoptotic Peptidase Activating Factor 1 (APAF1) ELISA Kit

Product Synonym Names
CED4; Apoptotic Protease Activating Factor
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
3D Structure
ModBase 3D Structure for Q9EPV5
Species Reactivity
Assay Type
Sandwich
Samples
Tissue Homogenates, Cell Lysates or Other Biological Fluids.
Detection Range
0.156-10ng/mL
Sensitivity
0.062ng/mL
RTU ELISA Kit's Advantage
The Ready-To-Use (RTU) Kits require fewer steps in setup and take less time to run. Detection Solutions are pre-diluted and total incubation time is shorter than many other ELISA Kits.
Preparation and Storage
For unopened kits: All the reagents should be kept at 4 degree C upon receipt.
For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Product Note
Our RTU ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of APAF1 rtu elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
APAF1 rtu elisa kit
Test Principle: The microtiter plate provided in this kit has been pre-coated with an antibody specific to APAF1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to APAF1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain APAF1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of APAF1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Typical Testing Data/Standard Curve (for reference only) of APAF1 rtu elisa kit
APAF1 rtu elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS457672. Click to request current manual
NCBI/Uniprot data below describe general gene information for APAF1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Related Accession #
Molecular Weight
141,152 Da
NCBI Official Full Name
apoptotic protease-activating factor 1
NCBI Official Synonym Full Names
apoptotic peptidase activating factor 1
NCBI Official Symbol
Apaf1  [Similar Products]
NCBI Protein Information
apoptotic protease-activating factor 1
UniProt Protein Name
Apoptotic protease-activating factor 1
UniProt Gene Name
Apaf1  [Similar Products]
UniProt Synonym Gene Names
APAF-1  [Similar Products]
NCBI Summary for APAF1
protein involved in the initiation of apoptosis [RGD, Feb 2006]
UniProt Comments for APAF1
Regulates progammed cell death; necessary for normal brain development. Participates with pro-caspase-9 (Apaf-3) in the cytochrome c-dependent activation of caspase-3, leading to apoptosis. This activation requires ATP ().
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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