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APC elisa kit :: Human Acti-vated protein C (APC) ELISA Kit

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Catalog # MBS268598
Unit / Price
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  48-Strip-Wells  /  $260 +1 FREE 8GB USB
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Typical Testing Data/Standard Curve (for reference only)
Product Name

Acti-vated protein C (APC), ELISA Kit

Full Product Name

Human Acti-vated protein C (APC) ELISA Kit

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
OMIM
gene 613659
Species Reactivity
Samples
Human serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant Acti-vated protein C (APC) concentration
Assay Type
Sandwich
Detection Range
10000 pg/ml-156 pg/ml
Sensitivity
5pg/ml.
Intra-assay Precision
<= 8%
Inter-assay Precision
<= 12%
Preparation and Storage
Store all reagents at 2-8 degree C.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of APC elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for APCpurchase
MBS268598 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Acti-vated protein C (APC) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing APC. The ELISA analytical biochemical technique of the MBS268598 kit is based on APC antibody-APC antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect APC antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, APC. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
APC elisa kit
Principle of the Assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is human APC monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

Typical Testing Data/Standard Curve (for reference only) of APC elisa kit
APC elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS268598. Click to request current manual
NCBI/Uniprot data below describe general gene information for APC. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
300,439 Da
NCBI Official Full Name
adenomatous polyposis coli protein isoform b
NCBI Official Synonym Full Names
adenomatous polyposis coli
NCBI Official Symbol
NCBI Official Synonym Symbols
GS; DP2; DP3; BTPS2; DP2.5; PPP1R46
  [Similar Products]
NCBI Protein Information
adenomatous polyposis coli protein; deleted in polyposis 2.5; adenomatosis polyposis coli tumor suppressor; protein phosphatase 1, regulatory subunit 46
UniProt Protein Name
Adenomatous polyposis coli protein
UniProt Synonym Protein Names
Deleted in polyposis 2.5
UniProt Gene Name
UniProt Synonym Gene Names
DP2.5; Protein APC  [Similar Products]
UniProt Entry Name
APC_HUMAN
NCBI Summary for APC
This gene encodes a tumor suppressor protein that acts as an antagonist of the Wnt signaling pathway. It is also involved in other processes including cell migration and adhesion, transcriptional activation, and apoptosis. Defects in this gene cause familial adenomatous polyposis (FAP), an autosomal dominant pre-malignant disease that usually progresses to malignancy. Disease-associated mutations tend to be clustered in a small region designated the mutation cluster region (MCR) and result in a truncated protein product. [provided by RefSeq, Jul 2008]
UniProt Comments for APC
APC: a tumor suppressor. Defects in APC are a cause of familial adenomatous polyposis (FAP). Promotes rapid degradation of CTNNB1 and participates in Wnt signaling. Activity is correlated with its phosphorylation state. Two splice-variant isoforms have been described.

Protein type: Motility/polarity/chemotaxis; Tumor suppressor

Chromosomal Location of Human Ortholog: 5q21-q22

Cellular Component: centrosome; tight junction; beta-catenin destruction complex; cytosol; nucleoplasm; kinetochore; cell-cell adherens junction; lamellipodium; cytoplasmic microtubule; cytoplasm; plasma membrane; nucleus; lateral plasma membrane

Molecular Function: microtubule plus-end binding; protein binding; cadherin binding; gamma-catenin binding; microtubule binding; beta-catenin binding; protein kinase regulator activity; protein kinase binding

Biological Process: positive regulation of cell adhesion; positive regulation of apoptosis; apoptosis; somatic stem cell maintenance; positive regulation of microtubule polymerization; T cell differentiation in the thymus; Wnt receptor signaling pathway through beta-catenin; chromosome organization and biogenesis; cytoplasmic microtubule organization and biogenesis; anterior/posterior pattern formation; muscle maintenance; negative regulation of cell proliferation; regulation of osteoclast differentiation; cytokinesis after mitosis; hair follicle development; mitotic cell cycle spindle assembly checkpoint; positive regulation of epithelial cell differentiation; protein complex assembly; cell cycle arrest; cell adhesion; kidney development; proximal/distal pattern formation; cell structure disassembly during apoptosis; regulation of microtubule-based process; regulation of attachment of spindle microtubules to kinetochore; negative regulation of odontogenesis; cell migration; regulation of osteoblast differentiation; thymus development; negative regulation of MAPKKK cascade; negative regulation of microtubule depolymerization; axis specification; negative regulation of cyclin-dependent protein kinase activity; positive regulation of pseudopodium formation; axonogenesis; dorsal/ventral pattern formation; retina development in camera-type eye; positive regulation of cell division; positive regulation of protein catabolic process; regulation of nitrogen compound metabolic process; response to DNA damage stimulus; mitotic metaphase/anaphase transition; positive regulation of cell migration

Disease: Familial Adenomatous Polyposis 1; Gastric Cancer; Desmoid Disease, Hereditary; Colorectal Cancer; Hepatocellular Carcinoma
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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