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Apo AI protein :: Apolipoprotein AI (Apo AI) Protein

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Catalog # MBS390131
Unit / Price
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  0.5 mg  /  $215 +1 FREE 8GB USB
  1 mg  /  $310 +1 FREE 8GB USB
Testing Data)
Product Name

Apolipoprotein AI (Apo AI), Protein

Popular Item
Full Product Name

Ultra Pure Human Apolipoprotein AI (Apo AI)

Product Synonym Names
Ultra Pure Human Apolipoprotein AI (Apo AI)
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Chromosome Location
Chromosome: 11; NC_000011.9 (116706467..116708338, complement). Location: 11q23-q24
OMIM
105200
3D Structure
ModBase 3D Structure for P02647
Purity/Purification
After series ultracentrifugations, High Density Lipoprotein (HDL) is isolated from human plasma. Apo AI is purified from delipidated HDL, followed by gel-filtration.
Purity: > 99 % by SDS-PAGE
Concentration
1mg / ml, determined by the Lowry method (lot specific)
Source Note
From fresh human plasma that has tested negative for Hepatitis C, HIV-I and HIV-II antibodies as well as Hepatitis surface antigens.
Buffer
20 mM Tris-HCl, 0.14 M NaCl, 0.02 % NaN3, 0.5 mM EDTA, pH 8.0.
Preparation and Storage
-20 degree C for long-term storage, 2-8 degree C for short-term storage. Aliquot to avoid repeated freezing and thawing.
Other Notes
Small volumes of Apo AI protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Product Categories/Family for Apo AI protein
Applications Tested/Suitable for Apo AI protein
SDS-PAGE
Application Notes for Apo AI protein
Purity > 99% by SDS-PAGE.

Testing Data) of Apo AI protein
Apo AI protein Testing Data) image
NCBI/Uniprot data below describe general gene information for Apo AI. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
30,778 Da
NCBI Official Full Name
apolipoprotein AI
NCBI Official Synonym Full Names
apolipoprotein A-I
NCBI Official Symbol
APOA1  [Similar Products]
NCBI Protein Information
apolipoprotein A-I; apo-AI
UniProt Protein Name
Apolipoprotein A-I
UniProt Synonym Protein Names
Apolipoprotein A1Cleaved into the following chain:Truncated apolipoprotein A-I; Alternative name(s):; Apolipoprotein A-I(1-242)
UniProt Gene Name
APOA1  [Similar Products]
UniProt Synonym Gene Names
Apo-AI; ApoA-I  [Similar Products]
UniProt Entry Name
APOA1_HUMAN
NCBI Summary for Apo AI
This gene encodes apolipoprotein A-I, which is the major protein component of high density lipoprotein (HDL) in plasma. The protein promotes cholesterol efflux from tissues to the liver for excretion, and it is a cofactor for lecithin cholesterolacyltransferase (LCAT) which is responsible for the formation of most plasma cholesteryl esters. This gene is closely linked with two other apolipoprotein genes on chromosome 11. Defects in this gene are associated with HDL deficiencies, including Tangier disease, and with systemic non-neuropathic amyloidosis. [provided by RefSeq, Jul 2008]
UniProt Comments for Apo AI
Function: Participates in the reverse transport of cholesterol from tissues to the liver for excretion by promoting cholesterol efflux from tissues and by acting as a cofactor for the lecithin cholesterol acyltransferase (LCAT). As part of the SPAP complex, activates spermatozoa motility. Ref.20

Subunit structure: Interacts with APOA1BP and CLU. Component of a sperm activating protein complex (SPAP), consisting of APOA1, an immunoglobulin heavy chain, an immunoglobulin light chain and albumin. Interacts with NDRG1. Ref.20 Ref.25 Ref.31 Ref.34

Subcellular location: Secreted.

Tissue specificity: Major protein of plasma HDL, also found in chylomicrons. Synthesized in the liver and small intestine. The oxidized form at Met-110 and Met-136 is increased in individuals with increased risk for coronary artery disease, such as in carrier of the eNOSa/b genotype and exposure to cigarette smoking. It is also present in increased levels in aortic lesions relative to native ApoA-I and increased levels are seen with increasing severity of disease. Ref.32

Post-translational modification: Palmitoylated. Ref.28Met-110 and Met-136 are oxidized to methionine sulfoxides.Phosphorylation sites are present in the extracellular medium.

Polymorphism: Genetic variations in APOA1 can result in APOA1 deficiency and are associated with low levels of HDL cholesterol [

MIM:107680].

Involvement in disease: High density lipoprotein deficiency 2 (HDLD2) [MIM:604091]: Inherited as autosomal dominant trait. It is characterized by moderately low HDL cholesterol, predilection toward premature coronary artery disease (CAD) and a reduction in cellular cholesterol efflux.Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.38 Ref.39High density lipoprotein deficiency 1 (HDLD1) [MIM:205400]: Recessive disorder characterized by absence of high density lipoprotein (HDL) cholesterol from plasma, accumulation of cholesteryl esters, premature coronary artery disease (CAD), hepatosplenomegaly, recurrent peripheral neuropathy and progressive muscle wasting and weakness.Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.38 Ref.39APOA1 mutations may be involved in the pathogenesis of amyloid polyneuropathy-nephropathy Iowa type, also known as amyloidosis van Allen type or familial amyloid polyneuropathy type III (Ref.44 and Ref.45). The clinical picture is dominated by neuropathy in the early stages of the disease and nephropathy late in the course. Death is due in most cases to renal amyloidosis. Ref.38 Ref.39Amyloidosis 8 (AMYL8) [MIM:105200]: A hereditary generalized amyloidosis due to deposition of apolipoprotein A1, fibrinogen and lysozyme amyloids. Viscera are particularly affected. There is no involvement of the nervous system. Clinical features include renal amyloidosis resulting in nephrotic syndrome, arterial hypertension, hepatosplenomegaly, cholestasis, petechial skin rash.Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.38 Ref.39 Ref.44 Ref.45 Ref.47 Ref.52

Sequence similarities: Belongs to the apolipoprotein A1/A4/E family.

Mass spectrometry: Molecular mass is 28081 Da from positions 25 - 267. Determined by ESI. Without methionine sulfoxide. Ref.32Molecular mass is 28098 Da from positions 25 - 267. Determined by ESI. With 1 methionine sulfoxide, oxidation at Met-110. Ref.32Molecular mass is 28095 Da from positions 25 - 267. Determined by ESI. With 1 methionine sulfoxide, oxidation at Met-136. Ref.32Molecular mass is 28114 Da from positions 25 - 267. Determined by ESI. With 2 methionine sulfoxides, oxidation at Met-110 and Met-136. Ref.32
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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