• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

anti-Apo AII antibody :: Sheep Apolipoprotein AII Antibody

Scan QR to view Datasheet
Catalog # MBS390013
Unit / Price
Scan QR to view Datasheet
  0.5 mL  /  $295 +1 FREE 8GB USB
  1 mL  /  $445 +1 FREE 8GB USB
anti-Apo AII antibody
Product Name

Apolipoprotein AII (Apo AII), Antibody

Full Product Name

Biotinylated Sheep Anti-Human Apolipoprotein AII

Product Synonym Names
Biotinylated Sheep Anti-Human Apolipoprotein AII (Apo AII), Polyclonal Antibody
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Chromosome Location
Chromosome: 1; NC_000001.10 (161192083..161193418, complement). Location: 1q23.3
OMIM
107670
3D Structure
ModBase 3D Structure for P02652
Host
Sheep
Specificity
Specifically binds to human apo AII. Dilution for immunoblot and ELISA range: 200 to 10,000.
Concentration
1.2 mg / ml in freeze-dried powder (lot specific)
Source Note
Polyclonal antibody purified by human apo AII-Sepharose affinity column and labeled with biotin.
Preservative
75 mM PBS, 75 mM NaCl, 0.5 mM EDTA, 0.02% NaN3, pH 7.2.
Stabilizer
10 mg / ml Bovine Serum Albumin.
Preparation and Storage
Freeze-dried product should be refrigerated until opened. After opening, restore to 0.5 ml with distilled water. If it is not completely clear after standing for 1-2 hours at room temperature, centrifuge the product. It is stable for several weeks at 4o C as an undiluted liquid. Do not use for more than one day after dilution. For extended storage after reconstitution, we suggest the addition of an equal volume of glycerol to make a final glycerol concentration of 50% followed by storage at -20o C. The concentration of protein and buffer salts will decrease to one-half of the original after the addition of glycerol.
Other Notes
Small volumes of anti-Apo AII antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Product Categories/Family for anti-Apo AII antibody
Applications Tested/Suitable for anti-Apo AII antibody
ELISA (EIA), Immunoblot (IB)
Application Notes for anti-Apo AII antibody
Freeze-dried powder. Dilution for Immunoblot & ELISA range: 1,000 to 10,000.
Use: The antibody can be used for detection of apo AII in human plasma and lipoproteins, immunoassays and immunoblots.
NCBI/Uniprot data below describe general gene information for Apo AII. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
11,175 Da
NCBI Official Full Name
apolipoprotein A-II preproprotein
NCBI Official Synonym Full Names
apolipoprotein A-II
NCBI Official Symbol
APOA2  [Similar Products]
NCBI Official Synonym Symbols
apoAII; Apo-AII; ApoA-II
  [Similar Products]
NCBI Protein Information
apolipoprotein A-II; apolipoprotein A2
UniProt Protein Name
Apolipoprotein A-II
UniProt Synonym Protein Names
Apolipoprotein A2Cleaved into the following chain:Truncated apolipoprotein A-II; Alternative name(s):; Apolipoprotein A-II(1-76)
UniProt Gene Name
APOA2  [Similar Products]
UniProt Synonym Gene Names
Apo-AII; ApoA-II  [Similar Products]
UniProt Entry Name
APOA2_HUMAN
NCBI Summary for Apo AII
This gene encodes apolipoprotein (apo-) A-II, which is the second most abundant protein of the high density lipoprotein particles. The protein is found in plasma as a monomer, homodimer, or heterodimer with apolipoprotein D. Defects in this gene may result in apolipoprotein A-II deficiency or hypercholesterolemia. [provided by RefSeq, Jul 2008]
UniProt Comments for Apo AII
Function: May stabilize HDL (high density lipoprotein) structure by its association with lipids, and affect the HDL metabolism.

Subunit structure: Homodimer; disulfide-linked. Also forms a disulfide-linked heterodimer with APOD. Interacts with HCV core protein. Interacts with APOA1BP and NDRG1. Ref.15 Ref.16 Ref.19 Ref.23

Subcellular location: Secreted.

Tissue specificity: Plasma; synthesized in the liver and intestine.

Post-translational modification: Met-49 is oxidized to methionine sulfoxide.Phosphorylation sites are present in the extracellular medium.Apolipoprotein A-II is O-glycosylated. Ref.22

Polymorphism: A homozygous transition at position 1 of intron 3 of APOA2 results in deficiency of apolipoprotein A-II, without significant influence either on lipid and lipoprotein profiles or on the occurrence of coronary artery disease [

MIM:107670].

Sequence similarities: Belongs to the apolipoprotein A2 family.

Mass spectrometry: Molecular mass is 17252 Da from positions 24 - 100. Determined by ESI. Homodimer, without methionine sulfoxide. Ref.17Molecular mass is 17269 Da from positions 24 - 100. Determined by ESI. Homodimer, with 1 methionine sulfoxide, oxidation at Met-49. Ref.17Molecular mass is 8701.2 Da from positions 24 - 100. Determined by MALDI. Ref.18Molecular mass is 8823.4 Da from positions 24 - 100. Determined by MALDI. Cysteinylated ApoA-II. Ref.18Molecular mass is 17421.3 Da from positions 24 - 100. Determined by MALDI. Homodimer. Ref.18Molecular mass is 17293.4 Da from positions 24 - 100. Determined by MALDI. Heterodimer with truncated apolipoprotein A-II. Ref.18Molecular mass is 8578.3 Da from positions 24 - 99. Determined by MALDI. Ref.18Molecular mass is 17166.2 Da from positions 24 - 99. Determined by MALDI. Homodimer. Ref.18
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000