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APOA1 elisa kit :: Rabbit apolipoprotein A-I ELISA Kit

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Catalog # MBS704934
Unit / Price
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  24-Strip-Wells (LIMIT 1)  /  $240 +1 FREE 8GB USB
  48-Strip-Wells  /  $475 +1 FREE 8GB USB
  96-Strip-Wells  /  $675 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,715 +3 FREE 8GB USB
  10x96-Strip-Wells  /  $5,140 +6 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

apolipoprotein A-I (APOA1), ELISA Kit

Popular Item
Full Product Name

Rabbit apolipoprotein A1, Apo-A1 ELISA Kit

Product Synonym Names
Rabbit apolipoprotein A1 (Apo-A1) ELISA Kit; MGC117399; OTTHUMP00000069346; OTTHUMP00000069347; OTTHUMP00000069348; apolipoprotein A-I
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
MBS704934 Testing Data
3D Structure
ModBase 3D Structure for P09809
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of rabbit Apo-A1. No significant cross-reactivity or interference between rabbit Apo-A1 and analogues was observed.
Samples
Serum, plasma, tissue homogenates
Assay Type
Quantitative Sandwich
Detection Range
6.25 ug/ml - 400 ug/ml
Sensitivity
Typically less than 1.56 ug/ml.
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of APOA1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for APOA1purchase
MBS704934 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the apolipoprotein A-I (APOA1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing APOA1. The ELISA analytical biochemical technique of the MBS704934 kit is based on APOA1 antibody-APOA1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect APOA1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, APOA1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
APOA1 elisa kit
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for Apo-A1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any Apo-A1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for Apo-A1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of Apo-A1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Typical Testing Data/Standard Curve (for reference only) of APOA1 elisa kit
APOA1 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS704934. Click to request current manual
NCBI/Uniprot data below describe general gene information for APOA1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Related Accession #
Molecular Weight
30,591 Da
NCBI Official Full Name
apolipoprotein A-I preproprotein
NCBI Official Symbol
APOA1  [Similar Products]
NCBI Protein Information
apolipoprotein A-I; apo-AI; apoA-I; apolipoprotein A1
UniProt Protein Name
Apolipoprotein A-I
UniProt Synonym Protein Names
Apolipoprotein A1Cleaved into the following 2 chains:Proapolipoprotein A-I; ProapoA-I; Truncated apolipoprotein A-I
Protein Family
UniProt Gene Name
APOA1  [Similar Products]
UniProt Synonym Gene Names
Apo-AI; ApoA-I; ProapoA-I  [Similar Products]
UniProt Entry Name
APOA1_RABIT
UniProt Comments for APOA1
Function: Participates in the reverse transport of cholesterol from tissues to the liver for excretion by promoting cholesterol efflux from tissues and by acting as a cofactor for the lecithin cholesterol acyltransferase (LCAT). As part of the SPAP complex, activates spermatozoa motility.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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