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APOA2 elisa kit :: Human apolipoprotein A-II ELISA Kit

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Catalog # MBS704270
Unit / Price
  24-Strip-Wells (LIMIT 1)  /  $240 +1 FREE 8GB USB
  48-Strip-Wells  /  $550 +1 FREE 8GB USB
  96-Strip-Wells  /  $795 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,890 +3 FREE 8GB USB
  10x96-Strip-Wells  /  $5,465 +6 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

apolipoprotein A-II (APOA2), ELISA Kit

Also Known As

Human Apolipoprotein A2, apo-A2 ELISA Kit

Product Synonym Names
Human Apolipoprotein A2; apo-A2 ELISA Kit; apolipoprotein A-II
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
MBS704270 Testing Data
Sequence Length
100
OMIM
107670
3D Structure
ModBase 3D Structure for P02652
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of Human APOA2. No significant cross-reactivity or interference between Human APOA2 and analogues was observed.
Detection Range
6.2 ug/ml-400 ug/ml?
Sensitivity
1.6 ug/ml
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV% is less than 8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.
Detection Wavelength
450 nm
Sample Volume
50-100ul
Protein Biological Process 1
Immunity
Protein Biological Process 3
Host-virus interaction
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of APOA2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forAPOA2purchase
MBS704270 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the apolipoprotein A-II (APOA2) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing APOA2. The ELISA analytical biochemical technique of the MBS704270 kit is based on APOA2 antibody-APOA2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect APOA2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, APOA2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
APOA2 elisa kit
Introduction: Apolipoprotein A2 is a 17.5 kDa protein with a pI of 4.9. Lipids, as such areinsoluble in plasma. They combine with apolipoproteins to form lipoproteinsand this process solubilises lipids in plasma, providing a transport systemfor lipids. Apolipoproteins also play an important role in lipoprotein receptorrecognition and regulation of certain enzymes in lipoprotein metabolism.Two main forms of high density lipoprotein exist; both contain APOA1, butone type only contains APO A2.For more information on APO A2 pleasedownload the PDF catalogue below.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with anantibody specific to apo-A2. Standards or samples are then added to theappropriate microtiter plate wells with a biotin-conjugated polyclonalantibody preparation specific for apo-A2 and Avidin conjugated toHorseradish Peroxidase (HRP) is added to each microplate well andincubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solutionis added to each well. Only those wells that contain apo-A2,biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit achange in color. The enzyme-substrate reaction is terminated by theaddition of a sulphuric acid solution and the color change is measuredspectrophotometrically at a wavelength of 450 nm +/- 2 nm. Theconcentration of apo-A2 in the samples is then determined by comparingthe O.D. of the samples to the standard curve.

Typical Testing Data/Standard Curve (for reference only) of APOA2 elisa kit
APOA2 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS704270. Click to request current manual
NCBI/Uniprot data below describe general gene information for APOA2. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
11,175 Da
NCBI Official Full Name
apolipoprotein A-II preproprotein
NCBI Official Synonym Full Names
apolipoprotein A-II
NCBI Official Symbol
APOA2  [Similar Products]
NCBI Official Synonym Symbols
apoAII; Apo-AII; ApoA-II
  [Similar Products]
NCBI Protein Information
apolipoprotein A-II; apolipoprotein A2
UniProt Protein Name
Apolipoprotein A-II
UniProt Synonym Protein Names
Apolipoprotein A2Cleaved into the following 2 chains:Proapolipoprotein A-II; ProapoA-II; Truncated apolipoprotein A-IIAlternative name(s):Apolipoprotein A-II(1-76)
Protein Family
UniProt Gene Name
APOA2  [Similar Products]
UniProt Synonym Gene Names
Apo-AII; ApoA-II; ProapoA-II  [Similar Products]
UniProt Entry Name
APOA2_HUMAN
NCBI Summary for APOA2
This gene encodes apolipoprotein (apo-) A-II, which is the second most abundant protein of the high density lipoprotein particles. The protein is found in plasma as a monomer, homodimer, or heterodimer with apolipoprotein D. Defects in this gene may result in apolipoprotein A-II deficiency or hypercholesterolemia. [provided by RefSeq, Jul 2008]
UniProt Comments for APOA2
Function: May stabilize HDL (high density lipoprotein) structure by its association with lipids, and affect the HDL metabolism.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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