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APOB elisa kit :: Sheep apolipoprotein B (including Ag (x) antigen) (APOB) ELISA Kit

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Catalog # MBS707266 (SPECIAL PROMOTIONAL PRICING for a limited time)
Unit / Price
  24-Strip-Wells (LIMIT 1)  /  $240 +1 FREE 8GB USB
  48-Strip-Wells  /  $350 +1 FREE 8GB USB
  96-Strip-Wells  /  $495 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,105 +2 FREE 8GB USB
  10x96-Strip-Wells  /  $3,975 +4 FREE 8GB USB
APOB elisa kit
Product Name

apolipoprotein B (including Ag (x) antigen) (APOB), ELISA Kit

Popular Item
Also Known As

Sheep Apolipoprotein B, APOB ELISA Kit

Product Synonym Names
Sheep Apolipoprotein B (APOB) ELISA kit ; FLDB; LDLCQ4; apoB-100; apoB-48; apolipoprotein B; apolipoprotein B48; Sheep Apolipoprotein B (APOB)
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
Sequence Length
4563
OMIM
615558
3D Structure
ModBase 3D Structure for P04114
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of sheep APOB. No significant cross-reactivity or interference between sheep APOB and analogues was observed.
Samples
Serum, plasma.
Assay Type
Competitive
Detection Range
0.625 uU/ml -40 uU/ml.
Sensitivity
0.156 uU/ml.
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of APOB elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forAPOBpurchase
MBS707266 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the apolipoprotein B (including Ag (x) antigen) (APOB) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing APOB. The ELISA analytical biochemical technique of the MBS707266 kit is based on APOB antibody-APOB antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect APOB antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, APOB. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
APOB elisa kit
Principle of the Assay||This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with APOB. Standards or samples are added to the appropriate microtiter plate wells with Sheepradish Peroxidase (HRP) conjugated antibody preparation specific for APOB. The competitive inhibition reaction is launched between with pre-coated APOB and APOB in samples. A substrate solution is added to the wells and the color develops in opposite to the amount of APOB in the samples. The color development is stopped and the intensity of the color is measured.
Sample Manual Insert of MBS707266. Click to request current manual
NCBI/Uniprot data below describe general gene information for APOB. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
515,605 Da
NCBI Official Full Name
apolipoprotein B-100
NCBI Official Synonym Full Names
apolipoprotein B
NCBI Official Symbol
NCBI Official Synonym Symbols
FLDB; LDLCQ4
  [Similar Products]
NCBI Protein Information
apolipoprotein B-100; apoB-48; apoB-100; apo B-100; mutant Apo B 100; apolipoprotein B48; apolipoprotein B (including Ag(x) antigen)
UniProt Protein Name
Apolipoprotein B-100
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
Apo B-100; Apo B-48  [Similar Products]
UniProt Entry Name
APOB_HUMAN
NCBI Summary for APOB
This gene product is the main apolipoprotein of chylomicrons and low density lipoproteins. It occurs in plasma as two main isoforms, apoB-48 and apoB-100: the former is synthesized exclusively in the gut and the latter in the liver. The intestinal and the hepatic forms of apoB are encoded by a single gene from a single, very long mRNA. The two isoforms share a common N-terminal sequence. The shorter apoB-48 protein is produced after RNA editing of the apoB-100 transcript at residue 2180 (CAA->UAA), resulting in the creation of a stop codon, and early translation termination. Mutations in this gene or its regulatory region cause hypobetalipoproteinemia, normotriglyceridemic hypobetalipoproteinemia, and hypercholesterolemia due to ligand-defective apoB, diseases affecting plasma cholesterol and apoB levels. [provided by RefSeq, Jul 2008]
UniProt Comments for APOB
Function: Apolipoprotein B is a major protein constituent of chylomicrons (apo B-48), LDL (apo B-100) and VLDL (apo B-100). Apo B-100 functions as a recognition signal for the cellular binding and internalization of LDL particles by the apoB/E receptor.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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