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ApoB48 elisa kit :: Rat apolipoprotein B-48 (ApoB48) ELISA Kit

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Catalog # MBS925341
Unit / Price
  24-Strip-Wells (LIMIT 1)  /  $240 +1 FREE 8GB USB
  48-Strip-Wells  /  $495 +1 FREE 8GB USB
  96-Strip-Wells  /  $710 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,705 +3 FREE 8GB USB
  10x96-Strip-Wells  /  $5,145 +6 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

apolipoprotein B-48 (ApoB48), ELISA Kit

Popular Item
Also Known As

Rat apolipoprotein B-48, ApoB48 ELISA Kit

Product Synonym Names
Rat apolipoprotein B-48 (ApoB48) ELISA kit; apolipoprotein B-48 (ApoB48)
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
MBS925341 Testing Data
Sequence Length
4506
3D Structure
ModBase 3D Structure for Q7TMA5
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of rat ApoB48. No significant cross-reactivity or interference between rat ApoB48 and analogues was observed.
Samples
Serum, plasma, cell culture supernates, tissue homogenates
Detection Range
0.312 ug/ml - 20 ug/mL
Sensitivity
<0.078 µg/mL
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of ApoB48 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forApoB48purchase
MBS925341 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the apolipoprotein B-48 (ApoB48) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing ApoB48. The ELISA analytical biochemical technique of the MBS925341 kit is based on ApoB48 antibody-ApoB48 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ApoB48 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ApoB48. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
ApoB48 elisa kit
Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with ApoB48. Standards or samples are added to the appropriate microtiter plate wells with Horseradish Peroxidase (HRP) conjugated antibody preparation specific for ApoB48. The competitive inhibition reaction is launched between with pre-coated ApoB48 and ApoB48 in samples. A substrate solution is added to the wells and the color develops in opposite to the amount of ApoB48 in the samples. The color development is stopped and the intensity of the color is measured.

Typical Testing Data/Standard Curve (for reference only) of ApoB48 elisa kit
ApoB48 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS925341. Click to request current manual
NCBI/Uniprot data below describe general gene information for ApoB48. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Related Accession #
Molecular Weight
536,024 Da
NCBI Official Full Name
apolipoprotein B-100
NCBI Official Synonym Full Names
apolipoprotein B
NCBI Official Symbol
NCBI Official Synonym Symbols
Aa1064; Ac1-060; ApoB-48; ApoB-100; Apo B-100
  [Similar Products]
NCBI Protein Information
apolipoprotein B-100; apolipoprotein B PI; apolipoprotein B-48; apolipoprotein B (including Ag(x) antigen)
UniProt Protein Name
Apolipoprotein B-100
UniProt Gene Name
UniProt Synonym Gene Names
Aa1064; Ac1-060; Apo B-100; Apo B-48  [Similar Products]
UniProt Entry Name
APOB_RAT
NCBI Summary for ApoB48
This gene product is the main apolipoprotein of chylomicrons and low density lipoproteins. It occurs in plasma as two main isoforms, apoB-L and apoB-H. Unlike the apoB-48 and apoB-100 structural equivalents in human, which are synthesized exclusively in the gut and liver, respectively, the rat apoBL isoform is also found in rat liver. The intestinal and the hepatic forms of apoB are encoded by a single gene from a single, very long mRNA. The two isoforms share a common N-terminal sequence. The shorter apoB-L protein is produced after RNA editing of the apoB-H transcript at residue 2180 (CAA->UAA), resulting in the creation of a stop codon, and early translation termination. [provided by RefSeq, Jul 2008]
UniProt Comments for ApoB48
APOB: Apolipoprotein B is a major protein constituent of chylomicrons (apo B-48), LDL (apo B-100) and VLDL (apo B-100). Apo B-100 functions as a recognition signal for the cellular binding and internalization of LDL particles by the apoB/E receptor. Defects in APOB are a cause of familial hypobetalipoproteinemia type 1 (FHBL1). A disorder characterized by highly reduced plasma concentrations of low density lipoproteins, and dietary fat malabsorption. Clinical presentation may vary from no symptoms to severe gastrointestinal and neurological dysfunction similar to abetalipoproteinemia. Defects in APOB are a cause of familial ligand-defective apolipoprotein B-100 (FDB). FDB is a dominantly inherited disorder of lipoprotein metabolism leading to hypercholesterolemia and increased proneness to coronary artery disease (CAD). The plasma cholesterol levels are dramatically elevated due to impaired clearance of LDL particles by defective APOB/E receptors. Defects in APOB associated with defects in other genes (polygenic) can contribute to hypocholesterolemia.

Protein type: Secreted, signal peptide; Carrier; Secreted

Cellular Component: Golgi apparatus; extracellular space; chylomicron; cell soma; intracellular membrane-bound organelle; endoplasmic reticulum; cytoplasm; plasma membrane; vesicle membrane; actin cytoskeleton

Molecular Function: heparin binding; lipid transporter activity; protein binding; low-density lipoprotein receptor binding; cholesterol transporter activity; phospholipid binding; lipid binding

Biological Process: lipoprotein catabolic process; response to lipopolysaccharide; response to carbohydrate stimulus; post-embryonic development; response to organic substance; response to selenium ion; triacylglycerol catabolic process; nervous system development; cholesterol metabolic process; cholesterol transport; in utero embryonic development; regulation of cholesterol biosynthetic process; response to virus; cholesterol efflux; lipoprotein metabolic process; lipid transport; sperm motility; cholesterol homeostasis; fertilization; lipoprotein transport; lipoprotein biosynthetic process; artery morphogenesis; spermatogenesis; lipid metabolic process; triacylglycerol mobilization; lipid catabolic process
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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