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APOL1 elisa kit :: Rat Apolipoprotein L1 ELISA Kit

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Catalog # MBS739571
Unit / Price
  48-Strip-Wells  /  $440 +1 FREE 8GB USB
  96-Strip-Wells  /  $640 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,895 +3 FREE 8GB USB
  10x96-Strip-Wells  /  $5,415 +6 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

Apolipoprotein L1 (APOL1), ELISA Kit

Popular Item
Also Known As

Rat Apolipoprotein L1 ELISA Kit

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
OMIM
gene 612551
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of ApoL1. No significant cross-reactivity or interference between ApoL1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between ApoL1 and all the analogues, therefore, cross reaction may still exist in some cases.
Samples
Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type
Sandwich
Detection Range
2.5-50ng/mL
Sensitivity
0.1ng/mL
Intended Uses
This ApoL1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat ApoL1. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
Preparation and Storage
Store all reagents at 2-8 degree C.
Sample Preparation
We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of APOL1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forAPOL1purchase
MBS739571 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Apolipoprotein L1 (APOL1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing APOL1. The ELISA analytical biochemical technique of the MBS739571 kit is based on APOL1 antibody-APOL1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect APOL1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, APOL1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
APOL1 elisa kit
Principle of the assay: ApoL1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ApoL1 antibody and an ApoL1-HRP conjugate. The assay sample and buffer are incubated together with ApoL1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ApoL1 concentration since ApoL1 from samples and ApoL1-HRP conjugate compete for the anti-ApoL1 antibody binding site. Since the number of sites is limited, as more sites are occupied by ApoL1 from the sample, fewer sites are left to bind ApoL1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ApoL1 concentration in each sample is interpolated from this standard curve.
Product Categories/Family for APOL1 elisa kit

Typical Testing Data/Standard Curve (for reference only) of APOL1 elisa kit
APOL1 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS739571. Click to request current manual
NCBI/Uniprot data below describe general gene information for APOL1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
42,158 Da
NCBI Official Full Name
apolipoprotein L1 isoform a
NCBI Official Synonym Full Names
apolipoprotein L, 1
NCBI Official Symbol
APOL1  [Similar Products]
NCBI Official Synonym Symbols
APOL; APO-L; FSGS4; APOL-I
  [Similar Products]
NCBI Protein Information
apolipoprotein L1
UniProt Protein Name
Apolipoprotein L1
UniProt Synonym Protein Names
Apolipoprotein L; Apo-L; ApoL; Apolipoprotein L-I; ApoL-I
Protein Family
UniProt Gene Name
APOL1  [Similar Products]
UniProt Synonym Gene Names
APOL; Apo-L; ApoL; ApoL-I  [Similar Products]
UniProt Entry Name
APOL1_HUMAN
NCBI Summary for APOL1
This gene encodes a secreted high density lipoprotein which binds to apolipoprotein A-I. Apolipoprotein A-I is a relatively abundant plasma protein and is the major apoprotein of HDL. It is involved in the formation of most cholesteryl esters in plasma and also promotes efflux of cholesterol from cells. This apolipoprotein L family member may play a role in lipid exchange and transport throughout the body, as well as in reverse cholesterol transport from peripheral cells to the liver. Several different transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2008]
UniProt Comments for APOL1
APOL1: May play a role in lipid exchange and transport throughout the body. May participate in reverse cholesterol transport from peripheral cells to the liver. Defects in APOL1 are the cause of focal segmental glomerulosclerosis type 4 (FSGS4). It is a renal pathology defined by the presence of segmental sclerosis in glomeruli and resulting in proteinuria, reduced glomerular filtration rate and edema. Renal insufficiency often progresses to end-stage renal disease, a highly morbid state requiring either dialysis therapy or kidney transplantation. Belongs to the apolipoprotein L family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Channel, chloride; Secreted; Lipid-binding; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 22q13.1

Cellular Component: extracellular space; extracellular region; intrinsic to membrane

Molecular Function: chloride channel activity; protein binding; lipid binding

Biological Process: receptor-mediated endocytosis; cholesterol metabolic process; killing of cells of another organism; cytolysis; innate immune response; lipoprotein metabolic process; chloride transport; lipid transport

Disease: Focal Segmental Glomerulosclerosis 4, Susceptibility To
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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