• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

anti-APP antibody :: Rabbit APP (CT) Antibody

Scan QR to view Datasheet
Catalog # MBS194025
Unit / Price
  0.1 mg  /  $400 +1 FREE 8GB USB
anti-APP antibody
Product Name

APP (CT), Antibody

Popular Item
Also Known As

Anti-APP (CT) Antibody

Product Synonym Names
Anti-APP (CT); APP (CT)
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Chromosome Location
Chromosome: 21; NC_000021.8 (27252861..27543138, complement). Location: 21q21.2; 21q21.3
OMIM
104300
3D Structure
ModBase 3D Structure for P05067
Host
Rabbit
Specificity
Anti-APP (CT) [Rabbit antibody to peptide corresponding to aa 737-751 of human amyloid protein precursor (APP) or aa 85-99 of the C99 fragment generated by b-secretase cleavage.]
This antibody recognizes human, mouse, and rat APP and the C99 fragment.
Form/Format
100 ug IgG in PBS, pH 7.4, purified by immunoaffinity chromatography.
Antigen
Peptide corresponding to aa 737- 751 of human amyloid protein precursor (APP) or aa 85-99 of the C99 fragment generated by beta - secretase cleavage. The sequences are identical to those of monkey, mouse, rat, chicken, and a variety of other species.
Stabilizer
None
Preservatives
0.02% sodium azide.
Dilutions Instructions
Dilute in PBS or medium which is identical to that used in the assay system.
Preparation and Storage
This antibody is stable for at least one (1) year at -20 degree C. Avoid multiple freeze-thaw cycles.
Other Notes
Small volumes of anti-APP antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-APP antibody
Accumulation of the amyloid- beta peptide (A beta ) in the cerebral cortex is a critical event in the pathogenesis of Alzheimer's disease. The beta -amyloid protein precursor (APP) is cleaved by beta -secretase, producing a soluble derivative of the protein and a membrane anchored 99-amino acid carboxy-terminal fragment (C99). The C99 fragment serves as substrate for gamma -secretase to generate the 4 kD amyloid- beta peptide which is deposited in the brain.
Applications Tested/Suitable for anti-APP antibody
Immunoblot
Application Notes for anti-APP antibody
Immunoblotting: use at 1:500-1:1,000 dilution.
Positive control: Mouse brain lysate.
NCBI/Uniprot data below describe general gene information for APP. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
86,943 Da[Similar Products]
NCBI Official Full Name
amyloid protein
NCBI Official Synonym Full Names
amyloid beta (A4) precursor protein
NCBI Official Symbol
NCBI Official Synonym Symbols
AAA; AD1; PN2; ABPP; APPI; CVAP; ABETA; PN-II; CTFgamma
  [Similar Products]
NCBI Protein Information
amyloid beta A4 protein; preA4; protease nexin-II; OTTHUMP00000096095; OTTHUMP00000096096; OTTHUMP00000096097; OTTHUMP00000096098; OTTHUMP00000225525; peptidase nexin-II; beta-amyloid peptide; alzheimer disease amyloid protein; cerebral vascular amyloid peptide
UniProt Protein Name
Amyloid beta A4 protein
UniProt Synonym Protein Names
ABPP; APPI; APP; Alzheimer disease amyloid protein; Cerebral vascular amyloid peptide; CVAP; PreA4; Protease nexin-II; PN-IICleaved into the following 14 chains:N-APPSoluble APP-alpha; S-APP-alphaSoluble APP-beta; S-APP-betaC99Beta-amyloid protein 42; Alternative name(s):; Beta-APP42Beta-amyloid protein 40; Alternative name(s):; Beta-APP40C83P3(42)P3(40)C80Gamma-secretase C-terminal fragment 59; Alternative name(s):; Amyloid intracellular domain 59; AICD-59; AID(59); Gamma-CTF(59)Gamma-secretase C-terminal fragment 57; Alternative name(s):; Amyloid intracellular domain 57; AICD-57; AID(57); Gamma-CTF(57)Gamma-secretase C-terminal fragment 50; Alternative name(s):; Amyloid intracellular domain 50; AICD-50; AID(50); Gamma-CTF(50)
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
A4; AD1  [Similar Products]
UniProt Entry Name
A4_HUMAN
NCBI Summary for APP
This gene encodes a cell surface receptor and transmembrane precursor protein that is cleaved by secretases to form a number of peptides. Some of these peptides are secreted and can bind to the acetyltransferase complex APBB1/TIP60 to promote transcriptional activation, while others form the protein basis of the amyloid plaques found in the brains of patients with Alzheimer disease. Mutations in this gene have been implicated in autosomal dominant Alzheimer disease and cerebroarterial amyloidosis (cerebral amyloid angiopathy). Multiple transcript variants encoding several different isoforms have been found for this gene. [provided by RefSeq]
UniProt Comments for APP
APP: a cell surface receptor that influences neurite growth, neuronal adhesion and axonogenesis. Cleaved by secretases to form a number of peptides, some of which bind to the acetyltransferase complex Fe65/TIP60 to promote transcriptional activation. The Abeta peptide is released from the cell, its extracellular deposition and accumulation form the main components of amyloid plaques in Alzheimer's disease. Mutations in this gene have been implicated in autosomal dominant Alzheimer disease and cerebroarterial amyloidosis. Can promote transcription activation through binding to Fe65-Tip60 and inhibits Notch signaling through interaction with Numb. Couples to apoptosis-inducing pathways such as those mediated by G(O) and JIP. Inhibits G(O) alpha ATPase activity. Acts as a kinesin I membrane receptor, mediating the axonal transport of beta-secretase and presenilin 1. Involved in copper homeostasis/oxidative stress through copper ion reduction. In vitro, copper-metallated APP induces neuronal death directly or is potentiated through Cu(2+)-mediated low-density lipoprotein oxidation. Can regulate neurite outgrowth through binding to components of the extracellular matrix such as heparin and collagen I and IV. Induces a RAGE-dependent pathway that activates p38 MAPK, resulting in internalization of amyloid-beta peptide and leading to mitochondrial dysfunction in cultured cortical neurons. Provides Cu(2+) ions for GPC1 which are required for release of nitric oxide (NO) and subsequent degradation of the heparan sulfate chains on GPC1. Binds, via its C-terminus, to the PID domain of several cytoplasmic proteins, including APBB family members, the APBA family, JIP1, SHC1 and, NUMB and DAB1. Binding to DAB1 inhibits its serine phosphorylation. Associates with microtubules in the presence of ATP and in a kinesin-dependent manner. Amyloid beta-42 binds nAChRA7 in hippocampal neurons. Beta-amyloid associates with HADH2. Soluble APP binds, via its N-terminal head, to FBLN1. Expressed in all fetal tissues examined with highest levels in brain, kidney, heart and spleen. Weak expression in liver. In adult brain, highest expression found in the frontal lobe of the cortex and in the anterior perisylvian cortex- opercular gyri. Moderate expression in the cerebellar cortex, the posterior perisylvian cortex-opercular gyri and the temporal associated cortex. Weak expression found in the striate, extra- striate and motor cortices. Expressed in cerebrospinal fluid, and plasma. 10 isoforms of the human protein are produced by alternative splicing. Isoform APP695 is the predominant form in neuronal tissue, isoform APP751 and isoform APP770 are widely expressed in non- neuronal cells. Isoform APP751 is the most abundant form in T-lymphocytes. Appican is expressed in astrocytes. The splice isoforms that contain the BPTI domain possess protease inhibitor activity. Belongs to the APP family.

Protein type: Transcription factor; Apoptosis; Cell surface; Membrane protein, integral; Receptor, misc.

Chromosomal Location of Human Ortholog: 21q21.3

Cellular Component: Golgi apparatus; extracellular space; cell surface; intracellular membrane-bound organelle; integral to plasma membrane; dendritic spine; integral to membrane; extracellular region; coated pit; intercellular junction; cytosol; ER to Golgi transport vesicle; lipid raft; ciliary rootlet; nuclear envelope lumen; axon; apical part of cell; perinuclear region of cytoplasm; cytoplasm; plasma membrane; synapse; spindle midzone; dendritic shaft; neuromuscular junction; receptor complex; endosome

Molecular Function: serine-type endopeptidase inhibitor activity; heparin binding; identical protein binding; protein binding; enzyme binding; protease activator activity; DNA binding; transition metal ion binding; PTB domain binding; acetylcholine receptor binding; receptor binding

Biological Process: extracellular matrix organization and biogenesis; adult locomotory behavior; locomotory behavior; mRNA polyadenylation; positive regulation of mitotic cell cycle; protein amino acid phosphorylation; regulation of translation; platelet degranulation; synaptic growth at neuromuscular junction; forebrain development; dendrite development; visual learning; collateral sprouting in the absence of injury; neuromuscular process controlling balance; cell adhesion; neurite development; cholesterol metabolic process; platelet activation; Notch signaling pathway; cellular copper ion homeostasis; regulation of epidermal growth factor receptor activity; axon cargo transport; mating behavior; regulation of multicellular organism growth; endocytosis; axon midline choice point recognition; smooth endoplasmic reticulum calcium ion homeostasis; neuron apoptosis; negative regulation of neuron differentiation; axonogenesis; suckling behavior; ionotropic glutamate receptor signaling pathway; regulation of protein binding; regulation of synapse structure and activity; innate immune response; positive regulation of transcription from RNA polymerase II promoter; response to oxidative stress; blood coagulation; neuron remodeling

Disease: Alzheimer Disease
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000