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APP elisa kit :: Mouse amyloid beta (A4) precursor protein ELISA Kit

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Catalog # MBS945014
Unit / Price
  24-Strip-Wells (LIMIT 1)  /  $240 +1 FREE 8GB USB
  48-Strip-Wells  /  $435 +1 FREE 8GB USB
  96-Strip-Wells  /  $625 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,595 +3 FREE 8GB USB
  10x96-Strip-Wells  /  $4,905 +6 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

amyloid beta (A4) precursor protein (APP), ELISA Kit

Full Product Name

Mouse Amyloid beta A4 protein, APP ELISA Kit

Product Synonym Names
Mouse Amyloid beta A4 protein (APP) ELISA kit; AAA; ABETA; ABPP; AD1; APPI; CTFgamma; CVAP; PN2; OTTHUMP00000096096; amyloid beta A4 protein; beta-amyloid peptide; cerebral vascular amyloid peptide; peptidase nexin-II; protease nexin-II; amyloid beta (A4) precursor protein
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
MBS945014 Testing Data
3D Structure
ModBase 3D Structure for P12023
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of mouse APP. No significant cross-reactivity or interference between mouse APP and analogues was observed.
Samples
Serum, plasma, tissue homogenates
Assay Type
Sandwich
Detection Range
15.6 ng/ml -1000 ng/ml.
Sensitivity
The minimum detectable dose of mouse APP is typically less than 3.9 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of APP elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forAPPpurchase
MBS945014 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the amyloid beta (A4) precursor protein (APP) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing APP. The ELISA analytical biochemical technique of the MBS945014 kit is based on APP antibody-APP antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect APP antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, APP. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
APP elisa kit
Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for APP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any APP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for APP is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of APP bound in the initial step. The color development is stopped and the intensity of the color is measured.

Typical Testing Data/Standard Curve (for reference only) of APP elisa kit
APP elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS945014. Click to request current manual
NCBI/Uniprot data below describe general gene information for APP. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
86,722 Da
NCBI Official Full Name
amyloid beta A4 protein isoform 1
NCBI Official Synonym Full Names
amyloid beta (A4) precursor protein
NCBI Official Symbol
NCBI Official Synonym Symbols
Ag; Abpp; Adap; Cvap; Abeta; betaApp; E030013M08Rik
  [Similar Products]
NCBI Protein Information
amyloid beta A4 protein; appican; protease nexin II; amyloidogenic glycoprotein; alzheimer disease amyloid A4 protein homolog
UniProt Protein Name
Amyloid beta A4 protein
UniProt Synonym Protein Names
ABPP; APP; Alzheimer disease amyloid A4 protein homolog; Amyloidogenic glycoprotein; AG
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
APP; AG; S-APP-alpha; S-APP-beta; AID(59); AID(57); AID(50)  [Similar Products]
UniProt Entry Name
A4_MOUSE
UniProt Comments for APP
APP: a cell surface receptor that influences neurite growth, neuronal adhesion and axonogenesis. Cleaved by secretases to form a number of peptides, some of which bind to the acetyltransferase complex Fe65/TIP60 to promote transcriptional activation. The Abeta peptide is released from the cell, its extracellular deposition and accumulation form the main components of amyloid plaques in Alzheimer's disease. Mutations in this gene have been implicated in autosomal dominant Alzheimer disease and cerebroarterial amyloidosis. Can promote transcription activation through binding to Fe65-Tip60 and inhibits Notch signaling through interaction with Numb. Couples to apoptosis-inducing pathways such as those mediated by G(O) and JIP. Inhibits G(O) alpha ATPase activity. Acts as a kinesin I membrane receptor, mediating the axonal transport of beta-secretase and presenilin 1. Involved in copper homeostasis/oxidative stress through copper ion reduction. In vitro, copper-metallated APP induces neuronal death directly or is potentiated through Cu(2+)-mediated low-density lipoprotein oxidation. Can regulate neurite outgrowth through binding to components of the extracellular matrix such as heparin and collagen I and IV. Induces a RAGE-dependent pathway that activates p38 MAPK, resulting in internalization of amyloid-beta peptide and leading to mitochondrial dysfunction in cultured cortical neurons. Provides Cu(2+) ions for GPC1 which are required for release of nitric oxide (NO) and subsequent degradation of the heparan sulfate chains on GPC1. Binds, via its C-terminus, to the PID domain of several cytoplasmic proteins, including APBB family members, the APBA family, JIP1, SHC1 and, NUMB and DAB1. Binding to DAB1 inhibits its serine phosphorylation. Associates with microtubules in the presence of ATP and in a kinesin-dependent manner. Amyloid beta-42 binds nAChRA7 in hippocampal neurons. Beta-amyloid associates with HADH2. Soluble APP binds, via its N-terminal head, to FBLN1. Expressed in all fetal tissues examined with highest levels in brain, kidney, heart and spleen. Weak expression in liver. In adult brain, highest expression found in the frontal lobe of the cortex and in the anterior perisylvian cortex- opercular gyri. Moderate expression in the cerebellar cortex, the posterior perisylvian cortex-opercular gyri and the temporal associated cortex. Weak expression found in the striate, extra- striate and motor cortices. Expressed in cerebrospinal fluid, and plasma. 10 isoforms of the human protein are produced by alternative splicing. Isoform APP695 is the predominant form in neuronal tissue, isoform APP751 and isoform APP770 are widely expressed in non- neuronal cells. Isoform APP751 is the most abundant form in T-lymphocytes. Appican is expressed in astrocytes. The splice isoforms that contain the BPTI domain possess protease inhibitor activity. Belongs to the APP family.

Protein type: Receptor, misc.; Apoptosis; Transcription factor; Cell surface; Membrane protein, integral

Cellular Component: Golgi apparatus; extracellular space; cell surface; smooth endoplasmic reticulum; integral to membrane; terminal button; coated pit; intercellular junction; ER to Golgi transport vesicle; lipid raft; ciliary rootlet; membrane; nuclear envelope lumen; perinuclear region of cytoplasm; cytoplasm; cytoplasmic vesicle; intracellular; neuromuscular junction; endosome; receptor complex; neuron projection; intracellular membrane-bound organelle; cell; axon; apical part of cell; plasma membrane; spindle midzone

Molecular Function: heparin binding; serine-type endopeptidase inhibitor activity; identical protein binding; protease activator activity; protease inhibitor activity; metal ion binding; PTB domain binding; acetylcholine receptor binding; protein binding; enzyme binding; DNA binding; transition metal ion binding; receptor binding

Biological Process: extracellular matrix organization and biogenesis; adult locomotory behavior; apoptosis; negative regulation of peptidase activity; locomotory behavior; mRNA polyadenylation; positive regulation of mitotic cell cycle; protein amino acid phosphorylation; regulation of translation; synaptic growth at neuromuscular junction; forebrain development; dendrite development; collateral sprouting in the absence of injury; visual learning; neuromuscular process controlling balance; cell adhesion; neurite development; protein homooligomerization; nervous system development; cholesterol metabolic process; Notch signaling pathway; cellular copper ion homeostasis; regulation of epidermal growth factor receptor activity; mating behavior; axon cargo transport; regulation of multicellular organism growth; endocytosis; axon midline choice point recognition; smooth endoplasmic reticulum calcium ion homeostasis; negative regulation of neuron differentiation; neuron apoptosis; suckling behavior; axonogenesis; regulation of gene expression; ionotropic glutamate receptor signaling pathway; regulation of synapse structure and activity; regulation of protein binding; positive regulation of transcription from RNA polymerase II promoter; response to oxidative stress; neuron remodeling
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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