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AQP1 elisa kit :: Human Aquaporin 1, Colton Blood Group ELISA Kit

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Catalog # MBS726672
Unit / Price
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  48-Strip-Wells  /  $440 +1 FREE 8GB USB
  96-Strip-Wells  /  $640 +1 FREE 8GB USB
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Typical Testing Data/Standard Curve
Product Name

Aquaporin 1, Colton Blood Group (AQP1), ELISA Kit

Popular Item
Full Product Name

Human Aquaporin 1, Colton Blood Group ELISA Kit

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
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Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of AQP-1. No significant cross-reactivity or interference between AQP-1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between AQP-1 and all the analogues, therefore, cross reaction may still exist in some cases.
Samples
Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type
Sandwich
Detection Range
0.5-10ng/mL
Sensitivity
0.1ng/mL
Preparation and Storage
Store all reagents at 2-8 degree C.
Sample Preparation
We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
Product Note
Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of AQP1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for AQP1purchase
MBS726672 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Aquaporin 1, Colton Blood Group (AQP1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing AQP1. The ELISA analytical biochemical technique of the MBS726672 kit is based on AQP1 antibody-AQP1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect AQP1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, AQP1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
AQP1 elisa kit
Intended Uses: This AQP-1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human AQP-1. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Principle of the Assay||AQP-1 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for AQP-1. Standards or samples are then added to the microtiter plate wells and AQP-1 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of AQP-1 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for AQP-1 are added to each well to "sandwich" the AQP-1 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain AQP-1 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The AQP-1 concentration in each sample is interpolated from this standard curve.
Product Categories/Family for AQP1 elisa kit

Typical Testing Data/Standard Curve of AQP1 elisa kit
AQP1 elisa kit Typical Testing Data/Standard Curve image
Sample Manual Insert of MBS726672. Click to request current manual
NCBI/Uniprot data below describe general gene information for AQP1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
28,857 Da
NCBI Official Full Name
aquaporin-1
NCBI Official Synonym Full Names
aquaporin 1
NCBI Official Symbol
NCBI Official Synonym Symbols
CHIP28
  [Similar Products]
NCBI Protein Information
aquaporin-1; AQP-1; aquaporin-CHIP; Aquaporin 1 (aquaporin channel forming integral protein 28 (CHIP)); water channel protein for red blood cells and kidney proximal tubule
UniProt Protein Name
Aquaporin-1
UniProt Synonym Protein Names
Aquaporin-CHIP; Water channel protein for red blood cells and kidney proximal tubule
UniProt Gene Name
UniProt Synonym Gene Names
Chip28; AQP-1  [Similar Products]
UniProt Entry Name
AQP1_RAT
NCBI Summary for AQP1
integral membrane protein that is a major water transport molecule in the kidney proximal tubule and red blood cells [RGD, Feb 2006]
UniProt Comments for AQP1
AQP1: Forms a water-specific channel that provides the plasma membranes of red cells and kidney proximal tubules with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient. Belongs to the MIP/aquaporin (TC 1.A.8) family.

Protein type: Membrane protein, integral; Transporter, aquaporin family; Transporter; Membrane protein, multi-pass

Cellular Component: neuron projection; nuclear membrane; cell; basolateral plasma membrane; brush border membrane; integral to plasma membrane; integral to membrane; membrane; axon; apical part of cell; apical plasma membrane; cytoplasm; plasma membrane; basal plasma membrane; nerve terminal; nucleus; sarcolemma; brush border

Molecular Function: protein binding; ephrin receptor binding; potassium channel activity; water transporter activity; intracellular cGMP activated cation channel activity; ammonium transmembrane transporter activity; glycerol transmembrane transporter activity; potassium ion transmembrane transporter activity; transmembrane transporter activity; nitric oxide transporter activity; water channel activity; glycerol channel activity

Biological Process: glycerol transport; cGMP biosynthetic process; cerebrospinal fluid secretion; wound healing; carbon dioxide transport; water transport; response to hormone stimulus; negative regulation of caspase activity; pancreatic juice secretion; sensory perception of pain; positive regulation of saliva secretion; water homeostasis; odontogenesis; positive regulation of fibroblast proliferation; nitric oxide transport; hyperosmotic salinity response; cellular homeostasis; multicellular organismal water homeostasis; potassium ion transport; lipid digestion; lateral ventricle development; secretion by cell; response to drug; cellular water homeostasis; secretory granule organization and biogenesis; glomerular filtration; ammonium transport; hyperosmotic response; renal water transport; positive regulation of angiogenesis; camera-type eye morphogenesis; adrenocorticotropin hormone secretion; response to estrogen stimulus; establishment and/or maintenance of actin cytoskeleton polarity; cell volume homeostasis; positive regulation of cell migration; negative regulation of apoptosis
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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