• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

ARC elisa kit :: Human Activity regulated cytoskeleton associated protein (ARC) ELISA Kit

Scan QR to view Datasheet
Catalog # MBS7241755
Unit / Price
Scan QR to view Datasheet
  48-Strip-Wells  /  $440 +1 FREE 8GB USB
  96-Strip-Wells  /  $640 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,895 +3 FREE 8GB USB
  10x96-Strip-Wells  /  $5,415 +6 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

Activity regulated cytoskeleton associated protein (ARC), ELISA Kit

Popular Item
Full Product Name

Human Activity regulated cytoskeleton associated protein (ARC) ELISA Kit

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
OMIM
612461
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of ARC. No significant cross-reactivity or interference between ARC and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between ARC and all the analogues, therefore, cross reaction may still exist in some cases.
Samples
Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type
Competitive
Detection Range
1.0-25ng/mL
Sensitivity
0.1ng/mL
Preparation and Storage
Store all reagents at 2-8 degree C.
Sample Preparation
We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of ARC elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for ARCpurchase
MBS7241755 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Activity regulated cytoskeleton associated protein (ARC) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing ARC. The ELISA analytical biochemical technique of the MBS7241755 kit is based on ARC antibody-ARC antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ARC antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ARC. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
ARC elisa kit
Intended Uses: This ARC ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human ARC. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Principle of the Assay: ARC ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ARC antibody and an ARC-HRP conjugate. The assay sample and buffer are incubated together with ARC-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ARC concentration since ARC from samples and ARC-HRP conjugate compete for the anti-ARC antibody binding site. Since the number of sites is limited, as more sites are occupied by ARC from the sample, fewer sites are left to bind ARC-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ARC concentration in each sample is interpolated from this standard curve.
Product Categories/Family for ARC elisa kit

Typical Testing Data/Standard Curve (for reference only) of ARC elisa kit
ARC elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS7241755. Click to request current manual
NCBI/Uniprot data below describe general gene information for ARC. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
45,316 Da
NCBI Official Full Name
activity-regulated cytoskeleton-associated protein
NCBI Official Synonym Full Names
activity-regulated cytoskeleton-associated protein
NCBI Official Symbol
NCBI Official Synonym Symbols
Arg3.1
  [Similar Products]
NCBI Protein Information
activity-regulated cytoskeleton-associated protein; ARC/ARG3.1; activity-regulated gene 3.1 protein homolog
UniProt Protein Name
Activity-regulated cytoskeleton-associated protein
UniProt Synonym Protein Names
ARC/ARG3.1; Activity-regulated gene 3.1 protein homolog; Arg3.1
UniProt Gene Name
UniProt Synonym Gene Names
KIAA0278; Arg3.1  [Similar Products]
UniProt Entry Name
ARC_HUMAN
UniProt Comments for ARC
ARG3.1: Required for consolidation of synaptic plasticity as well as formation of long-term memory. Regulates endocytosis of AMPA receptors in response to synaptic activity. Required for homeostatic synaptic scaling of AMPA receptors. Plays a role in the regulation of cell morphology and cytoskeletal organization. Required in the stress fiber dynamics and cell migration. Belongs to the ARC/ARG3.1 family.

Chromosomal Location of Human Ortholog: 8q24.3

Cellular Component: postsynaptic membrane; postsynaptic density; cytoplasm; acrosome; plasma membrane; dendritic spine; cell junction; endosome; actin cytoskeleton

Biological Process: anterior/posterior pattern formation; cell migration; regulation of cell morphogenesis; endocytosis; cytoskeleton organization and biogenesis; learning; endoderm development; regulation of neuronal synaptic plasticity
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Products associated with ARC elisa kit
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000