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AREG elisa kit :: Chicken Amphiregulin ELISA Kit

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Catalog # MBS740233
Unit / Price
  48-Strip-Wells  /  $440 +1 FREE 8GB USB
  96-Strip-Wells  /  $640 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,895 +3 FREE 8GB USB
  10x96-Strip-Wells  /  $5,415 +6 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

Amphiregulin (AREG), ELISA Kit

Also Known As

Chicken Amphiregulin ELISA Kit

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
OMIM
104640
Species Reactivity
Samples
Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type
Competitive
Sensitivity
1.0 pg/mL.
Intended Uses
This AREG ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Chicken AREG. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
Preparation and Storage
Store all reagents at 2-8 degree C.
Sample Preparation
We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of AREG elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forAREGpurchase
MBS740233 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Amphiregulin (AREG) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing AREG. The ELISA analytical biochemical technique of the MBS740233 kit is based on AREG antibody-AREG antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect AREG antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, AREG. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
AREG elisa kit
Principle of the Assay: AREG ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-AREG antibody and an AREG-HRP conjugate. The assay sample and buffer are incubated together with AREG-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the AREG concentration since AREG from samples and AREG-HRP conjugate compete for the anti-AREG antibody binding site. Since the number of sites is limited, as more sites are occupied by AREG from the sample, fewer sites are left to bind AREG-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The AREG concentration in each sample is interpolated from this standard curve.
Product Categories/Family for AREG elisa kit

Typical Testing Data/Standard Curve (for reference only) of AREG elisa kit
AREG elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS740233. Click to request current manual
NCBI/Uniprot data below describe general gene information for AREG. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
27,895 Da
NCBI Official Full Name
amphiregulin
NCBI Official Synonym Full Names
amphiregulin
NCBI Official Symbol
NCBI Official Synonym Symbols
AR; SDGF; AREGB; CRDGF
  [Similar Products]
NCBI Protein Information
amphiregulin; amphiregulin B; schwannoma-derived growth factor; colorectum cell-derived growth factor
UniProt Protein Name
Amphiregulin
UniProt Synonym Protein Names
Colorectum cell-derived growth factor; CRDGF
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
SDGF
AREGB; AR; CRDGF  [Similar Products]
UniProt Entry Name
AREG_HUMAN
NCBI Summary for AREG
The protein encoded by this gene is a member of the epidermal growth factor family. It is an autocrine growth factor as well as a mitogen for astrocytes, Schwann cells and fibroblasts. It is related to epidermal growth factor (EGF) and transforming growth factor alpha (TGF-alpha). The protein interacts with the EGF/TGF-alpha receptor to promote the growth of normal epithelial cells, and it inhibits the growth of certain aggressive carcinoma cell lines. It also functions in mammary gland, oocyte and bone tissue development. This gene is associated with a psoriasis-like skin phenotype, and is also associated with other pathological disorders, including various types of cancers and inflammatory conditions. [provided by RefSeq, Apr 2014]
UniProt Comments for AREG
AREG: Ligand of the EGF receptor/EGFR. Autocrine growth factor as well as a mitogen for a broad range of target cells including astrocytes, Schwann cells and fibroblasts. Belongs to the amphiregulin family.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 4q13.3

Cellular Component: extracellular space; cell surface; cytoplasm; integral to membrane; nucleus

Molecular Function: protein binding; growth factor activity; cytokine activity; epidermal growth factor receptor binding

Biological Process: epidermal growth factor receptor signaling pathway; response to peptide hormone stimulus; response to cAMP; response to glucocorticoid stimulus; response to estradiol stimulus; glial cell proliferation; G-protein coupled receptor protein signaling pathway; cell proliferation; response to hydrogen peroxide; cell-cell signaling; positive regulation of cell proliferation; negative regulation of osteoblast differentiation; neurite development; positive regulation of DNA replication; positive regulation of phosphorylation
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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