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Arg1 elisa kit :: Monkey Arginase 1 (Liver-type) ELISA Kit

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Catalog # MBS748033
Unit / Price
  48-Strip-Wells  /  $440 +1 FREE 8GB USB
  96-Strip-Wells  /  $640 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,895 +3 FREE 8GB USB
  10x96-Strip-Wells  /  $5,415 +6 FREE 8GB USB
Typical Standard Curve/Testing Data
Product Name

Arginase 1 (Liver-type) (Arg1), ELISA Kit

Popular Item
Also Known As

Monkey Arginase 1 (Liver-type) ELISA Kit

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
OMIM
608313
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of L-ARG1. No significant cross-reactivity or interference between L-ARG1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between L-ARG1 and all the analogues, therefore, cross reaction may still exist in some cases.
Samples
Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type
Competitive
Detection Range
5-100ng/mL
Sensitivity
1.0ng/mL
Preparation and Storage
Store all reagents at 2-8 degree C.
Sample Preparation
We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of Arg1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forArg1purchase
MBS748033 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Arginase 1 (Liver-type) (Arg1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Arg1. The ELISA analytical biochemical technique of the MBS748033 kit is based on Arg1 antibody-Arg1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Arg1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, Arg1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
Arg1 elisa kit
Intended Uses: This L-ARG1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Monkey L-ARG1. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Principle of the Assay: L-ARG1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-L-ARG1 antibody and an L-ARG1-HRP conjugate. The assay sample and buffer are incubated together with L-ARG1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the L-ARG1 concentration since L-ARG1 from samples and L-ARG1-HRP conjugate compete for the anti-L-ARG1 antibody binding site. Since the number of sites is limited, as more sites are occupied by L-ARG1 from the sample, fewer sites are left to bind L-ARG1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The L-ARG1 concentration in each sample is interpolated from this standard curve.
Product Categories/Family for Arg1 elisa kit

Typical Standard Curve/Testing Data of Arg1 elisa kit
Arg1 elisa kit Typical Standard Curve/Testing Data image
Sample Manual Insert of MBS748033. Click to request current manual
NCBI/Uniprot data below describe general gene information for Arg1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
25,356 Da
NCBI Official Full Name
arginase-1 isoform 1
NCBI Official Synonym Full Names
arginase 1
NCBI Official Symbol
NCBI Protein Information
arginase-1; arginase, liver; type I arginase; liver-type arginase
UniProt Protein Name
Arginase-1
UniProt Synonym Protein Names
Liver-type arginase; Type I arginase
Protein Family
UniProt Gene Name
UniProt Entry Name
ARGI1_HUMAN
NCBI Summary for Arg1
Arginase catalyzes the hydrolysis of arginine to ornithine and urea. At least two isoforms of mammalian arginase exist (types I and II) which differ in their tissue distribution, subcellular localization, immunologic crossreactivity and physiologic function. The type I isoform encoded by this gene, is a cytosolic enzyme and expressed predominantly in the liver as a component of the urea cycle. Inherited deficiency of this enzyme results in argininemia, an autosomal recessive disorder characterized by hyperammonemia. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011]
UniProt Comments for Arg1
ARG1: Homotrimer. By arginine or homoarginine. Belongs to the arginase family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.5.3.1; Hydrolase; Amino Acid Metabolism - arginine and proline

Chromosomal Location of Human Ortholog: 6q23

Cellular Component: extracellular space; neuron projection; cell soma; cytoplasm; cytosol; nucleus

Molecular Function: manganese ion binding; arginase activity

Biological Process: response to drug; mammary gland involution; maternal process involved in pregnancy; response to herbicide; response to amino acid stimulus; liver development; response to vitamin A; response to manganese ion; response to selenium ion; response to vitamin E; response to cadmium ion; response to zinc ion; response to methylmercury; arginine catabolic process; positive regulation of endothelial cell proliferation; response to axon injury; collagen biosynthetic process; lung development; response to amine stimulus; urea cycle

Disease: Argininemia
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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