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anti-ARHGDIA antibody :: ARHGDIA Antibody

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Catalog # MBS859500
Unit / Price
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  0.1 mg  /  $215 +1 FREE 8GB USB
Product Name

ARHGDIA, Antibody

Popular Item
Full Product Name

ARHGDIA (Phospho-Ser174) Antibody

Product Synonym Names
GDIR; Rho GDI 1; Rho GDP-dissociation inhibitor 1; Rho-GDI alpha
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
OMIM
601925
3D Structure
ModBase 3D Structure for P52565
Species Reactivity
Human, Mouse, Rat
Specificity
ARHGDIA (Phospho-Ser174) antibody detects endogenous levels of ARHGDIA only when phosphorylated at serine 174.
Purity/Purification
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Form/Format
In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Immunogen
The antiserum was produced against synthesized phosphopeptide derived from human ARHGDIA around the phosphorylation site of serine 174 (R-G-SP-Y-S).
All Sites
Human: Ser174
Mouse: Ser174
Rat: Ser174
Preparation and Storage
Store at-20 degree C for 1 year.
Other Notes
Small volumes of anti-ARHGDIA antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-ARHGDIA antibody
Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
Application Notes for anti-ARHGDIA antibody
WB: 1:500~1:1000
IHC: 1:50~1:100
ELISA: 1:10000

Testing Data of anti-ARHGDIA antibody
P-peptide-+
anti-ARHGDIA antibody Testing Data image
Immunohistochemistry (IHC) of anti-ARHGDIA antibody
Immunohistochemistry analysis of paraffin-embedded human cervix carcinoma tissue using ARHGDIA (Phospho-Ser174) antibody. Western blot analysis of extracts from HUVEC cells, treated with EGF (200ng/ml, 30mins), using ARHGDIA (Phospho-Ser174) antibody.
anti-ARHGDIA antibody Immunohistochemistry (IHC) (IHC) image
NCBI/Uniprot data below describe general gene information for ARHGDIA. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
18,233 Da
NCBI Official Full Name
rho GDP-dissociation inhibitor 1 isoform a
NCBI Official Synonym Full Names
Rho GDP dissociation inhibitor alpha
NCBI Official Symbol
ARHGDIA  [Similar Products]
NCBI Official Synonym Symbols
GDIA1; NPHS8; RHOGDI; RHOGDI-1; HEL-S-47e
  [Similar Products]
NCBI Protein Information
rho GDP-dissociation inhibitor 1
UniProt Protein Name
Rho GDP-dissociation inhibitor 1
UniProt Synonym Protein Names
Rho-GDI alpha
UniProt Gene Name
ARHGDIA  [Similar Products]
UniProt Synonym Gene Names
GDIA1; Rho GDI 1  [Similar Products]
NCBI Summary for ARHGDIA
This gene encodes a protein that plays a key role in the regulation of signaling through Rho GTPases. The encoded protein inhibits the disassociation of Rho family members from GDP (guanine diphosphate), thereby maintaining these factors in an inactive state. Activity of this protein is important in a variety of cellular processes, and expression of this gene may be altered in tumors. Mutations in this gene have been found in individuals with nephrotic syndrome, type 8. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jul 2014]
UniProt Comments for ARHGDIA
Controls Rho proteins homeostasis. Regulates the GDP/GTP exchange reaction of the Rho proteins by inhibiting the dissociation of GDP from them, and the subsequent binding of GTP to them. Retains Rho proteins such as CDC42, RAC1 and RHOA in an inactive cytosolic pool, regulating their stability and protecting them from degradation. Actively involved in the recycling and distribution of activated Rho GTPases in the cell, mediates extraction from membranes of both inactive and activated molecules due its exceptionally high affinity for prenylated forms. Through the modulation of Rho proteins, may play a role in cell motility regulation. In glioma cells, inhibits cell migration and invasion by mediating the signals of SEMA5A and PLXNB3 that lead to inactivation of RAC1.
Product References and Citations for anti-ARHGDIA antibody
Leffers H., Exp. Cell Res. 209:165-174(1993). Maeda A., Submitted (APR-1993) to the EMBL/GenBank/DDBJ databases. Chuang T.H., Submitted (JUL-1992) to the EMBL/GenBank/DDBJ databases.

Precautions
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Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

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