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anti-AS160 antibody :: Rabbit anti-Human, mouse AS160 (Phospho-Thr642) Antibody

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Catalog # MBS002191
Unit / Price
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  0.1 mL  /  $440 +1 FREE 8GB USB
  0.2 mL  /  $715 +1 FREE 8GB USB
anti-AS160 antibody
Product Name

AS160 (Phospho-Thr642), Antibody

Popular Item
Full Product Name

Rabbit AS160 (Phospho-Thr642) Antibody

Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Chromosome Location
Chromosome: 13; NC_000013.10 (75858809..76056250, complement). Location: 13q22.2
OMIM
612465
3D Structure
ModBase 3D Structure for O60343
Host
Rabbit
Species Reactivity
Human, mouse
Specificity
Phospho-TBC1D4 (T642) Polyclonal Antibody dectects endogenous levels of TBC1D4 protein only when phosphorylated at T642.
Purity/Purification
The antibody was affinity-purified from rabbit anti-serum by affinity-chromatography using epitope-specific immunogen.
Form/Format
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% soidum azide.
Concentration
1 mg/ml (lot specific)
Immunogen
Synthesized peptide derived from human TBC1D4 around the phoshorylation site of T642.
Preparation and Storage
20 Degree C/ 1 year
Other Notes
Small volumes of anti-AS160 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-AS160 antibody
Immunofluorescence (IF), Immunohistochemistry (IHC), ELISA (EIA)
Application Notes for anti-AS160 antibody
Immunohistochemistry: 1/100-1/300
Immunofluorescence: 1/200-1/1000.
ELISA: 1/5000. Not yet tested in other applications.
NCBI/Uniprot data below describe general gene information for AS160. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
146,563 Da
NCBI Official Full Name
TBC1 domain family member 4
NCBI Official Synonym Full Names
TBC1 domain family, member 4
NCBI Official Symbol
TBC1D4  [Similar Products]
NCBI Official Synonym Symbols
AS160
  [Similar Products]
NCBI Protein Information
TBC1 domain family member 4; akt substrate of 160 kDa; Acrg embryonic lethality minimal region ortholog; TBC (Tre-2, BUB2, CDC16) domain-containing protein
UniProt Protein Name
TBC1 domain family member 4
UniProt Synonym Protein Names
Akt substrate of 160 kDa
UniProt Gene Name
TBC1D4  [Similar Products]
UniProt Synonym Gene Names
AS160; KIAA0603; AS160  [Similar Products]
UniProt Entry Name
TBCD4_HUMAN
NCBI Summary for AS160
This gene is a member of the Tre-2/BUB2/CDC16 domain family. The protein encoded by this gene is a Rab-GTPase-activating protein, and contains two phopshotyrosine-binding domains (PTB1 and PTB2), a calmodulin-binding domain (CBD), a Rab-GTPase domain, and multiple AKT phosphomotifs. This protein is thought to play an important role in glucose homeostasis by regulating the insulin-dependent trafficking of the glucose transporter 4 (GLUT4), important for removing glucose from the bloodstream into skeletal muscle and fat tissues. Reduced expression of this gene results in an increase in GLUT4 levels at the plasma membrane, suggesting that this protein is important in intracellular retention of GLUT4 under basal conditions. When exposed to insulin, this protein is phosphorylated, dissociates from GLUT4 vesicles, resulting in increased GLUT4 at the cell surface, and enhanced glucose transport. Phosphorylation of this protein by AKT is required for proper translocation of GLUT4 to the cell surface. Individuals homozygous for a mutation in this gene are at higher risk for type 2 diabetes and have higher levels of circulating glucose and insulin levels after glucose ingestion. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Aug 2015]
UniProt Comments for AS160
AS160: a widely-expressed Rab GTPase-activating protein which apparently plays a role in mediating insulin action. Phosphorylated by the Ser/Thr protein kinase Akt in response to insulin. Is redistributed from low-density microsomes to the cytosol upon insulin treatment. Insulin stimulation of GLUT4 exocytosis is dependent on AS160. The translocation of GLUT4 from intracellular storage sites to the cell surface functions to stimulate insulin uptake by adipose and muscle tissue. Its expression is decreased by twofold in pancreatic islets in type 2 diabetes patients compared to control subjects; its phosphorylation is impaired in skeletal muscle of type 2 diabetics. The protein is expressed in pancreatic Langerhans islets, including beta cells. Up-regulated in T cells from patients with atopic dermatitis. Two alternatively spliced isoforms have been described. Isoform 2, which promotes GLUT4 translocation at the plasma membrane, is the highest overexpressed in most tissues. Isoform 2 shows a cytoplasmic perinuclear localization in a myoblastic cell line in resting and insulin-stimulated cells Isoform 2 is strongly expressed in adrenal and thyroid gland, and also in lung, kidney, colon, brain and adipose tissue. Isoform 2 is moderately expressed in skeletal muscle. Isoform 1 is highly expressed in skeletal muscle and heart, but was not detectable in the liver or adipose tissue.

Protein type: GAPs; GAPs, Rab

Chromosomal Location of Human Ortholog: 13q22.2

Cellular Component: cytoplasmic vesicle membrane; cytoplasm

Biological Process: vesicle-mediated transport; negative regulation of vesicle fusion; cellular response to insulin stimulus

Disease: Diabetes Mellitus, Noninsulin-dependent, 5
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

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