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ASD elisa kit :: Rat Androstenedione (ASD) ELISA Kit

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Catalog # MBS260899
Unit / Price
  48-Strip-Wells  /  $275 +1 FREE 8GB USB
  96-Strip-Wells  /  $435 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $1,755 +2 FREE 8GB USB
  10x96-Strip-Wells  /  $3,150 +3 FREE 8GB USB
Typical Standard Curve/Testing Data
Product Name

Androstenedione (ASD), ELISA Kit

Popular Item
Also Known As

Rat Androstenedione (ASD) ELISA Kit

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
Sequence Length
323
OMIM
gene 614279
Species Reactivity
Specificity
No cross-reaction with other factors.
Samples
Serum, plasma or cell culture supernatant
Assay Type
Quantitative Sandwich
Detection Range
10 ng/ml-0.156 ng/ml
Sensitivity
Up to 0.05ng/ml.
Intra-assay Precision
<= 8%
Inter-assay Precision
<= 12%
Preparation and Storage
Store all reagents at 2-8 degree C.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of ASD elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forASDpurchase
MBS260899 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Androstenedione (ASD) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing ASD. The ELISA analytical biochemical technique of the MBS260899 kit is based on ASD antibody-ASD antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ASD antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ASD. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
ASD elisa kit
Principle of the Assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Rat ASD monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

Typical Standard Curve/Testing Data of ASD elisa kit
ASD elisa kit Typical Standard Curve/Testing Data image
Sample Manual Insert of MBS260899. Click to request current manual
NCBI/Uniprot data below describe general gene information for ASD. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
15,748 Da
NCBI Official Full Name
Aldo-keto reductase family 1 member C2
NCBI Official Synonym Full Names
aldo-keto reductase family 1, member C2
NCBI Official Symbol
AKR1C2  [Similar Products]
NCBI Official Synonym Symbols
DD; DD2; TDD; BABP; DDH2; HBAB; HAKRD; MCDR2; SRXY8; AKR1C-pseudo
  [Similar Products]
NCBI Protein Information
aldo-keto reductase family 1 member C2; DD-2; DD/BABP; 3-alpha-HSD3; pseudo-chlordecone reductase; type II dihydrodiol dehydrogenase; chlordecone reductase homolog HAKRD; testicular 17,20-desmolase deficiency; trans-1,2-dihydrobenzene-1,2-diol dehydrogenase; dihydrodiol dehydrogenase 2; bile acid binding protein; 3-alpha hydroxysteroid dehydrogenase, type III
UniProt Protein Name
Aldo-keto reductase family 1 member C2
UniProt Synonym Protein Names
3-alpha-HSD3; Chlordecone reductase homolog HAKRD; Dihydrodiol dehydrogenase 2; DD-2; DD2; Dihydrodiol dehydrogenase/bile acid-binding protein; DD/BABP; Trans-1,2-dihydrobenzene-1,2-diol dehydrogenase (EC:1.3.1.20); Type III 3-alpha-hydroxysteroid dehydrogenase (EC:1.1.1.357)
UniProt Gene Name
AKR1C2  [Similar Products]
UniProt Synonym Gene Names
DDH2; DD-2; DD2; DD/BABP  [Similar Products]
UniProt Entry Name
AK1C2_HUMAN
NCBI Summary for ASD
This gene encodes a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. These enzymes catalyze the conversion of aldehydes and ketones to their corresponding alcohols using NADH and/or NADPH as cofactors. The enzymes display overlapping but distinct substrate specificity. This enzyme binds bile acid with high affinity, and shows minimal 3-alpha-hydroxysteroid dehydrogenase activity. This gene shares high sequence identity with three other gene members and is clustered with those three genes at chromosome 10p15-p14. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Dec 2011]
UniProt Comments for ASD
AKR1C2: Works in concert with the 5-alpha/5-beta-steroid reductases to convert steroid hormones into the 3-alpha/5-alpha and 3-alpha/5-beta-tetrahydrosteroids. Catalyzes the inactivation of the most potent androgen 5-alpha-dihydrotestosterone (5-alpha- DHT) to 5-alpha-androstane-3-alpha,17-beta-diol (3-alpha-diol). Has a high bile-binding ability. Defects in AKR1C2 are a cause of 46,XY sex reversal type 8 (SRXY8). A disorder of sex development. Affected individuals have a 46,XY karyotype but present as phenotypically normal females. Belongs to the aldo/keto reductase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Xenobiotic Metabolism - metabolism by cytochrome P450; EC 1.3.1.20; EC 1.1.1.357; Oxidoreductase

Chromosomal Location of Human Ortholog: 10p15-p14

Cellular Component: cytoplasm

Molecular Function: oxidoreductase activity, acting on NADH or NADPH, quinone or similar compound as acceptor; ketosteroid monooxygenase activity; trans-1,2-dihydrobenzene-1,2-diol dehydrogenase activity; carboxylic acid binding; bile acid binding; aldehyde reductase activity; phenanthrene 9,10-monooxygenase activity

Biological Process: steroid metabolic process; G-protein coupled receptor protein signaling pathway; positive regulation of protein kinase B signaling cascade; epithelial cell differentiation; digestion; positive regulation of cell proliferation; progesterone metabolic process; prostaglandin metabolic process

Disease: 46,xy Sex Reversal 8
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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