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ASPH CT elisa kit :: Human C Terminal Aspartate beta Hydroxylase ELISA Kit

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Catalog # MBS748846
Unit / Price
  48-Strip-Wells  /  $440 +1 FREE 8GB USB
  96-Strip-Wells  /  $640 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,895 +3 FREE 8GB USB
  10x96-Strip-Wells  /  $5,415 +6 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

C Terminal Aspartate beta Hydroxylase (ASPH CT), ELISA Kit

Full Product Name

Human C Terminal Aspartate beta Hydroxylase ELISA Kit

Product Synonym Names
Human C Terminal Aspartate b Hydroxylase ELISA Kit
Product Gene Name
Product Synonym Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
OMIM
600582
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of ASPH-CT. No significant cross-reactivity or interference between ASPH-CT and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between ASPH-CT and all the analogues, therefore, cross reaction may still exist in some cases.
Samples
Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type
Competitive
Detection Range
250-5000 pg/mL
Sensitivity
1.0pg/mL
Intended Uses
This ASPH-CT ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human ASPH-CT. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
Preparation and Storage
Store all reagents at 2-8 degree C.
Sample Preparation
We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of ASPH CT elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forASPH CTpurchase
MBS748846 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the C Terminal Aspartate beta Hydroxylase (ASPH CT) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing ASPH CT. The ELISA analytical biochemical technique of the MBS748846 kit is based on ASPH CT antibody-ASPH CT antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ASPH CT antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ASPH CT. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
ASPH CT elisa kit
Principle of the assay: ASPH-CT ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for ASPH-CT. Standards or samples are then added to the microtiter plate wells and ASPH-CT if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of ASPH-CT present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for ASPH-CT are added to each well to "sandwich" the ASPH-CT immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain ASPH-CT and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ASPH-CT concentration in each sample is interpolated from this standard curve.

Typical Testing Data/Standard Curve (for reference only) of ASPH CT elisa kit
ASPH CT elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS748846. Click to request current manual
NCBI/Uniprot data below describe general gene information for ASPH CT. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
28,926 Da
NCBI Official Full Name
aspartyl/asparaginyl beta-hydroxylase isoform k
NCBI Official Synonym Full Names
aspartate beta-hydroxylase
NCBI Official Symbol
NCBI Official Synonym Symbols
AAH; BAH; HAAH; JCTN; FDLAB; junctin; CASQ2BP1
  [Similar Products]
NCBI Protein Information
aspartyl/asparaginyl beta-hydroxylase; humbug; junctate; A beta H-J-J; cardiac junctin; ASP beta-hydroxylase; peptide-aspartate beta-dioxygenase; aspartyl/asparaginyl-beta-hydroxylase
UniProt Protein Name
Aspartyl/asparaginyl beta-hydroxylase
UniProt Synonym Protein Names
Aspartate beta-hydroxylase; ASP beta-hydroxylase; Peptide-aspartate beta-dioxygenase
UniProt Gene Name
UniProt Synonym Gene Names
ASP beta-hydroxylase  [Similar Products]
UniProt Entry Name
ASPH_HUMAN
NCBI Summary for ASPH CT
This gene is thought to play an important role in calcium homeostasis. The gene is expressed from two promoters and undergoes extensive alternative splicing. The encoded set of proteins share varying amounts of overlap near their N-termini but have substantial variations in their C-terminal domains resulting in distinct functional properties. The longest isoforms (a and f) include a C-terminal Aspartyl/Asparaginyl beta-hydroxylase domain that hydroxylates aspartic acid or asparagine residues in the epidermal growth factor (EGF)-like domains of some proteins, including protein C, coagulation factors VII, IX, and X, and the complement factors C1R and C1S. Other isoforms differ primarily in the C-terminal sequence and lack the hydroxylase domain, and some have been localized to the endoplasmic and sarcoplasmic reticulum. Some of these isoforms are found in complexes with calsequestrin, triadin, and the ryanodine receptor, and have been shown to regulate calcium release from the sarcoplasmic reticulum. Some isoforms have been implicated in metastasis. [provided by RefSeq, Sep 2009]
UniProt Comments for ASPH CT
ASPH: Isoform 1: specifically hydroxylates an Asp or Asn residue in certain epidermal growth factor-like (EGF) domains of a number of proteins. Belongs to the aspartyl/asparaginyl beta-hydroxylase family. 9 isoforms of the human protein are produced by alternative splicing.

Protein type: Oxidoreductase; Membrane protein, integral; EC 1.14.11.16

Chromosomal Location of Human Ortholog: 8q12.1

Cellular Component: endoplasmic reticulum membrane; sarcoplasmic reticulum membrane; sarcoplasmic reticulum lumen; endoplasmic reticulum; integral to membrane; plasma membrane; integral to endoplasmic reticulum membrane

Molecular Function: protein binding; electron carrier activity; structural constituent of muscle; calcium ion binding; structural molecule activity; peptide-aspartate beta-dioxygenase activity

Biological Process: positive regulation of proteolysis; positive regulation of transcription, DNA-dependent; regulation of protein stability; palate development; pattern specification process; limb morphogenesis; response to ATP; peptidyl-aspartic acid hydroxylation; negative regulation of cell proliferation; activation of store-operated calcium channel activity; muscle contraction; regulation of inositol-1,4,5-triphosphate receptor activity; detection of calcium ion
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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