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Collagen-based Cell Contraction Assay Assay Kit

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Catalog # MBS168182
Unit / Price
  24 Assays  /  $470 +1 FREE 8GB USB
Product Name

Collagen-based Cell Contraction Assay, Assay Kit

Popular Item
Also Known As

Collagen-based Cell Contraction Assay

Product Synonym Names
Collagen-based Cell Contraction Assay
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
Preparation and Storage
Store all components at 4 degree C until their expiration dates.
Product Note
Our Assay Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of Collagen-based Cell Contraction Assay assay kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
Collagen-based Cell Contraction Assay assay kit
Background: Wound healing comprises of three processes: epithelialization, connective tissue deposition, and contraction. The contraction process is believed to be mediated by specialized fibroblasts called myofibroblasts. Three-dimensional collagen gels have been widely used in fibroblast contraction studies. There are several different culture models to study the ability of fibroblasts to reorganize and contract collagen matrices in vitro. In the floating contraction model, a freshly polymerized collagen matrix containing cells is released from the culture dish and allowed to float in culture medium, and contraction occurs in the absence of external mechanical load and without appearance of stress fibers in the cells. In the attached model, a polymerized collagen matrix containing cells remains attached to the culture dish during contraction. Mechanical tension develops during contraction, and cellular stress fibers assemble. The two-step model combines an initial period of attached matrix contraction leading to mechanical loading, followed by release of the matrices, resulting in mechanical unloading and further contraction as mechanical stress dissipates. The signaling mechanisms used by fibroblasts to regulate collagen matrix contraction depend on whether the cells are mechanically loaded or unloaded at the time that contraction is initiated as well as on the growth factor used to initiate contraction. For instance, stimulation of fibroblasts by lysophosphatidic acid (LPA) but not by platelet-derived growth factor (PDGF) causes robust force generation in restrained matrices, whereas LPA and PDGF stimulate floating matrix contraction equally well. 3D collagen matrix has also been used in the studies of integrin signaling, cell apoptosis and cytoskeleton reorganization. Since three-dimensional matrix adhesions differ in structure, localization, and function from two-dimensional adhesions; and therefore, three-dimensional cell-matrix interactions may be more relevant biologically. Collagen-based Contraction Assay Kit provides a simple system to assess cell contractivity in vitro and screen cell contraction mediators. Each kit provides sufficient quantities to perform up to 24 assays in a 24-well plate. The kit can be also used in culturing cells in 3D collagen matrix.

Sample Manual Insert of MBS168182. Click to request current manual
Product References and Citations for Collagen-based Cell Contraction Assay assay kit
1. Martin, P. (1997) Science 276, 75-81
2. Bell, E., Ivarsson, B., and Merrill, C. (1979) Proc. Natl. Acad. Sci. U. S. A. 76, 1274-1278
3. Stupak, D., and Harris, A. K. (1982) Dev. Biol. 90, 383-398
4. Mochitate, K., Pawelek, P., and Grinnell, F. (1991) Exp. Cell Res. 193, 198-207
5. Tian, B., Lessan, K., Kahm, J., Kleidon, J., and Henke, C. (2002) J. Biol. Chem. 277, 24667-24675
1. Woeller, C. F. et al. (2015). Salinomycin and other polyether ionophores are a new class of antiscarring agent. J Biol Chem. 290:3563-3575.
2. Luo, Y. et al. (2015). Magnitude-dependent proliferation and contractility modulation of human bladder smooth muscle cells under physiological stretch. World J Urol. doi:10.1007/s00345-015-1509-4.
3. Nie, L. et al. (2014). Endothelial-mesenchymal transition in normal human esophageal endothelial cells cocultured with esophageal adenocarcinoma cells: role of IL-1beta and TGF-beta2. Am J Physiol Cell Physiol. 307:C859-C877.
4. Kotio, K.U. et al. (2011). Implication of microRNAs in Atrial Natriuretic Peptide and Nitric Oxide Signaling in Vascular Smooth Muscle Cells. Am J Physiol Cell Physiol. 301:C929-C937.
5. Schell, C. et al. (2010). 15-deoxy-delta12-14-prostaglandin-J2 induces hypertrophy and loss of contractility in human testicular peritubular cells: implications for human male fertility. Endocrinology 151:1257-1268.

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