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CytoSelect 24-well Cell Haptotaxis Assay (8 um), COL-coated, Colorimetric Assay Kit

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Catalog # MBS168697
Unit / Price
  12 Assays  /  $550 +1 FREE 8GB USB
Product Name

CytoSelect 24-well Cell Haptotaxis Assay (8 um), COL-coated, Colorimetric, Assay Kit

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Also Known As

CytoSelect 24-well Cell Haptotaxis Assay (8 um), COL-coated, Colorimetric

Product Synonym Names
CytoSelect 24-well Cell Haptotaxis Assay (8 um), COL-coated, Colorimetric
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
Preparation and Storage
Store all components at 4 degree C until their expiration dates.
Product Note
Our Assay Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of CytoSelect 24-well Cell Haptotaxis Assay (8 um), COL-coated, Colorimetric assay kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Principle of the assay: The CytoSelect Cell Haptotaxis Assay Kit contains polycarbonate membrane inserts (8 mum pore size) in a 24-well plate. The membrane serves as a barrier to discriminate migratory cells from non-migratory cells. Migratory cells are able to extend protrusions towards the gradient of extracellular matrix density (via actin cytoskeleton reorganization) and ultimately pass through the pores of the polycarbonate membrane. Finally, the cells are removed from the top of the membrane and the migratory cells are stained and quantified.

Background: Cell migration is a highly integrated, multistep process that orchestrates embryonic morphogenesis, tissue repair and regeneration. It plays a pivotal role in the disease progression of cancer, mental retardation, atherosclerosis, and arthritis. The initial response of a cell to a migration-promoting agent is to polarize and extend protrusions in the direction of the attractant; these protrusions can consist of large, broad lamellipodia or spike-like filopodia. In either case, these protrusions are driven by actin polymerization and can be stabilized by extracellular matrix (ECM) adhesion or cell-cell interactions (via transmembrane receptors). CytoSelect Cell Haptotaxis Assay Kit utilizes polycarbonate membrane inserts (8 mum pore size) to assay the migratory properties of cells, the bottom side of the insert is coated with Collagen I. The kit contains sufficient reagents for the evaluation of 12 samples. The 8 mum pore size is optimal for epithelial and fibroblast cell migration. However, in the case of leukocyte chemotaxis, a smaller pore size (3 mum) is recommended.

Sample Manual Insert of MBS168697. Click to request current manual
1. Ridley AJ, Schwartz MA, Burridge K, Firtel RA, Ginsberg MH, Borisy G, Parsons JT, Horwitz AR. (2003) Science 302, 1704-9.
2. Horwitz R, Webb D. (2003) Curr Biol. 13, R756-9.
3. Lauffenburger DA, Horwitz AF. (1996) Cell 84, 359-369.
1. Herrera, I. et al. (2013). Matrix metalloproteinase (MMP)-1 induces lung alveolar epithelial cell migration and proliferation, protects from apoptosis, and represses mitochondrial oxygen consumption. J. Biol. Chem. 288:25964-25975.
2. Niccoli, S. et al. (2012).The Asian-American E6 variant protein of human papillomavirus 16 alone is sufficient to promote immortalization, transformation, and migration of primary human foreskin keratinocytes. J Virol. 86:12384-12396.3. Kamiya, K. et al. (2007). Protein Kinase C delta activated adhesion regulates vascular smooth muscle cell migration. J. Surg. Res. 141:91-96.

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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