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CytoSelect 48-well Cell Adhesion Assay (ECM Array, Colorimetric) Assay Kit

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Catalog # MBS168967
Unit / Price
  48 Assays  /  $570 +1 FREE 8GB USB
  240 Assays  /  $2,255 +2 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

CytoSelect 48-well Cell Adhesion Assay (ECM Array, Colorimetric), Assay Kit

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Full Product Name

CytoSelect 48-well Cell Adhesion Assay (ECM Array, Colorimetric)

Product Synonym Names
CytoSelect 48-well Cell Adhesion Assay (ECM Array, Colorimetric)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
Preparation and Storage
Store all kit components at 4 degree C up to their expiration dates.
Product Note
Our Assay Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of CytoSelect 48-well Cell Adhesion Assay (ECM Array, Colorimetric) assay kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Principle of the Assay: The CytoSelectTM Cell Adhesion Assay Kit utilizes an ECM protein-coated 48-well plate (see Adhesion Plate Layout below). First, cells are seeded onto the coated substrate, where the adherent cells captured. Next, unbound cells are washed away, and the adherent cells are fixed/stained. Finally, the stain is extracted and quantified colorimetrically.

Background/Introduction: Cell adhesion is a complex process involved in embryogenesis, migration/invasion, tissue remodeling, and wound healing. To perform these processes, cells adhere to extracellular matrix components (via adhesion receptors), forming complexes with components of the cytoskeleton that ultimately affect cell motility, differentiation, proliferation, and survival. The CytoSelect TM Cell Adhesion Assay Kit provides a rapid, quantitative method for evaluating cell adhesion. The kit contains sufficient reagents for the evaluation of 48 samples (40 ECM protein-coated wells, 8 BSA-coated wells).

Typical Testing Data/Standard Curve (for reference only) of CytoSelect 48-well Cell Adhesion Assay (ECM Array, Colorimetric) assay kit
CytoSelect 48-well Cell Adhesion Assay (ECM Array, Colorimetric) assay kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS168967. Click to request current manual
1. Hynes, R. O. (1992) Cell 69:11-25.
2. Schwartz, M. A., Schaller, M. D. and Ginsberg, M. H. (1995) Annu. Rev. Cell Dev. Biol. 11:549-599.
1. Tanoury, Z, et al. (2014). Genes involved in cell adhesion and signaling: a new repertoire of retinoic acid receptor target genes in mouse embryonic fibroblasts. J. Cell Sci. 127:521-533.
2. Xing, C. et al. (2013). Reversing effect of ring finger protein 43 inhibition on malignant phenotypes of human hepatocellular carcinoma. Mol. Cancer Ther. 12:94-103.
3. Mei, J. et al. (2012). Inhibition of IDO1 suppresses cyclooxygenase-2 and matrix metalloproteinase-9 expression and decreases proliferation, adhesion and invasion of endometrial stromal cells. Mol. Human. Reprod. 10.1093/molehr/gas021.
4. Kandalam, V. et al. (2011). Lack of tissue inhibitor of metalloproteinases 2 leads to exacerbated left ventricular dysfunction and adverse extracellular matrix remodeling in response to biomechanical stress. Circulation 124:2094-2105.
5. Tsukamoto, H. et al. (2011). Evaluation of anticancer activities of benzo[c]phenanthridine alkaloid sanguinarine in oral squamous cell carcinoma cell line. Anticancer Res. 31:2841-2846.
6. Cervera, A.M. et al. (2008). Cells silenced for SDHB expression display characteristic features of the tumor phenotype. Cancer Res. 68:4058-4067.

Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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